FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_2.3410000000475e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_2.3410000000475e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56580
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAC300.00511136729.9941063
CAAAGAC300.00511136729.9941063
GTATCAA551.3235278E-424.60591
CAACGCA551.3476699E-424.5406325
AACGCAG551.3476699E-424.5406326
TCAACGC551.3476699E-424.5406324
ACGCAGA654.175514E-420.7651487
CATCTCC1301.1586963E-920.76514845
ATCAACG654.175514E-420.7651483
AGAGTAC706.880854E-419.28192311
TATCAAC750.001094024517.9964642
CGCAGAG750.001094024517.9964648
CAGAGTA750.001094024517.99646410
GAGTACG800.001686058216.87168512
ACATCTC1601.5248588E-816.87168544
GCAGAGT800.001686058216.8716859
AGTACGG800.001686058216.87168513
GTACGGG900.003700304314.99705314
CACATCT2601.0913936E-1114.70864745
ACACATC3203.3833203E-1011.95077744
Produced by FastQC (version 0.11.2)