FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_19.341000000048fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_19.341000000048fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534334
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG250.002105542236.0019543
AACCCGT406.1598414E-428.12652226
ATACCGT406.1632146E-428.123896
TGTTCCG500.00226355522.4991115
GTAGTAC550.00393472420.4537373
CTCCGAG4700.020.10935245
ATTGTTA807.5556236E-519.68672215
TTTTGCG1151.5920159E-719.5644452
CATCTCC24400.019.55206545
GGTCCGA951.3077777E-518.9572641
CATGATA600.006507527518.749264
CGGGGGT600.006507527518.7492618
CCTAGAC851.20327495E-418.5286793
CTAGACA1153.4429759E-617.6084
ACTTAGC901.8638837E-417.499318
TTTGCGT1305.7706893E-717.3070093
GTATCAA15100.017.2937971
GTCCGAA1053.1084397E-517.1421812
TAAGGTG1205.164613E-616.8743345
TTGCGTT1358.564821E-716.6660084