FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_18.341000000048e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_18.341000000048e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458673
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC654.8637776E-727.6915723
GTATCAA5400.027.091471
CGGGCTT507.150478E-526.9992817
CGGGGGG608.179219E-626.249317
GGTATCA2250.025.0075131
AACCCCG450.001224842225.002067
TAGGACA751.6943322E-623.999364
ACCGTCC702.6967035E-522.5018528
CATCTCC13100.021.47123345
TCAACGC6400.021.0931874
TATCAAC6600.020.7948992
ATCTCCG4250.020.6487645
CTAGGAC1201.1012162E-820.624453
CAACGCA6500.020.4247575
AACGCAG6500.020.4247576
ATCAACG6800.019.8524113
TAATACA807.551901E-519.6869754
ACGCAGA6800.019.5236667
CAGTCCG706.9708494E-419.2873029
CGCAGAG6950.019.1022938
Produced by FastQC (version 0.11.2)