FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_17.341000000048c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_17.341000000048c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325429
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA5100.15671621152386542No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA4460.13704986341106662No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC3890.1195345221231052No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC3550.10908677468818083No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG3390.10417018765998114No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3310.10171189414588128No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTA250.002104999535.99963431
ACGAAAC250.002104999535.99963424
TAAACGA300.00514215729.99969321
GTTCCGT300.00514215729.99969330
GTAAACG300.00514215729.99969320
TAGGTTG406.156939E-428.1247125
TGCGTTC406.156939E-428.12471212
CGTTCCC450.001224738924.99974314
CTAATCT450.001224738924.9997434
GGCGTCA551.3653554E-424.54520412
GTTCATA500.002257246522.5066871
TACTGGT500.002259263822.503232
CGGGTGT500.002261282922.49977118
GGGGGTA1003.6245183E-822.49977118
TCTCCGA9650.021.68371645
AAAAGTA5200.021.637722
TCGCTCC754.589985E-520.99978431
TGTAGGA654.225146E-420.7722112
ACAGTTC654.2295316E-420.7690188
CCTTGCG654.2295316E-420.76901815