H23NVBCXX l02n01 6h_15.341000000048ad.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 6h_15.341000000048ad.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1402
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	70	4.9928673323823105	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	65	4.636233951497861	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	34	2.4251069900142657	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	30	2.1398002853067046	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	25	1.783166904422254	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	14	0.9985734664764622	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	11	0.7845934379457917	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	7	0.4992867332382311	RNA PCR Primer, Index 27 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT	7	0.4992867332382311	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT	5	0.3566333808844508	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG	4	0.28530670470756064	No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT	3	0.21398002853067047	Illumina PCR Primer Index 8 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT	3	0.21398002853067047	Illumina PCR Primer Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.21398002853067047	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	3	0.21398002853067047	TruSeq Adapter, Index 12 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.21398002853067047	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC	2	0.14265335235378032	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG	2	0.14265335235378032	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2	0.14265335235378032	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC	2	0.14265335235378032	No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCA	2	0.14265335235378032	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.14265335235378032	No Hit
GCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA	2	0.14265335235378032	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC	2	0.14265335235378032	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC	2	0.14265335235378032	RNA PCR Primer, Index 46 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCCAC	20	4.885333E-4	45.0	17
GTATGCC	20	4.885333E-4	45.0	42
ATCTCGT	20	4.885333E-4	45.0	37
GCCCACG	20	4.885333E-4	45.0	18
TGCCGTC	20	4.885333E-4	45.0	45
CCACGAG	20	4.885333E-4	45.0	20
TATGCCG	20	4.885333E-4	45.0	43
GAGCCCA	20	4.885333E-4	45.0	16
CACGAGA	20	4.885333E-4	45.0	21
CCCACGA	20	4.885333E-4	45.0	19
TCTCGTA	20	4.885333E-4	45.0	38
ACGAGAC	20	4.885333E-4	45.0	22
ATGCCGT	20	4.885333E-4	45.0	44
CGTATGC	20	4.885333E-4	45.0	41
CGAGCCC	20	4.885333E-4	45.0	15
CCGAGCC	20	4.885333E-4	45.0	14
TCGTATG	20	4.885333E-4	45.0	40
TCTCCGA	20	4.885333E-4	45.0	11
TCCGAGC	20	4.885333E-4	45.0	13
CTCGTAT	20	4.885333E-4	45.0	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content