FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_14.34100000004890.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_14.34100000004890.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425522
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC7820.18377428194076922No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAA250.002104702936.00319711
TGCCGTC1400.033.74902745
GCCCACG1600.030.93661318
AATCTCG1350.029.9991436
TCGTATG1650.028.63554240
ATGCCGT1650.028.63554244
CTCGTAT1700.027.7933239
TATGCCG1700.027.7933243
CACGAGA1750.026.99922621
CCACGAG1750.026.99922620
CGAGACA1500.026.99922423
CCCACGA1800.026.24924519
TCTCGTA1850.025.53980638
ACGAGAC1850.025.53980622
TCGTCCC450.001225490524.9992813
CGAGCCC2000.024.7492915
ACGCCTA551.3664924E-424.54474821
GGTATCA4000.024.198181
GTATCAA7950.023.784081
CGGGGTG952.1082087E-823.6835317
Produced by FastQC (version 0.11.2)