FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_13.34100000004883.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_13.34100000004883.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC492.318977756743966No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC452.1296734500709893No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC321.5144344533838145No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC221.0411736867013723RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC210.9938476100331283No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC120.5679129200189305No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT80.37860861334595364No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT60.28395646000946523Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC50.236630383341221TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC50.236630383341221No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC40.18930430667297682No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.14197823000473261No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC30.14197823000473261No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG205.544108E-445.036
GTATGCC301.3032004E-644.99999642
ATCTCGT301.3032004E-644.99999637
GCCCACG301.3032004E-644.99999618
TGCCGTC301.3032004E-644.99999645
CCACGAG301.3032004E-644.99999620
TATGCCG301.3032004E-644.99999643
CACGAGA301.3032004E-644.99999621
CCCACGA301.3032004E-644.99999619
TCTCGTA301.3032004E-644.99999638
ACGAGAC301.3032004E-644.99999622
ATGCCGT301.3032004E-644.99999644
CGTATGC301.3032004E-644.99999641
TCGTATG301.3032004E-644.99999640
CTCGTAT301.3032004E-644.99999639
AGCCCAC353.7721766E-638.5714317
GAGCCCA353.7721766E-638.5714316
CGAGCCC353.7721766E-638.5714315
CCGAGCC353.7721766E-638.5714314
TCCGAGC353.7721766E-638.5714313
Produced by FastQC (version 0.11.2)