FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_12.34100000004876.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_12.34100000004876.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683242
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG551.126773E-732.726635
GCGGTAT300.005137104530.0103951
GTATCAA10650.027.4743061
TATGCCG1150.027.3907743
ACGATTA551.3675909E-424.54497340
AATCTCG1201.6370905E-1124.37452136
TGCCGTC1301.8189894E-1224.23029945
CGAGACA1252.910383E-1123.39954423
CCCACGA1500.022.49956119
GCCTATC500.002263933722.4995617
TATCAAC13150.022.0718142
ACGGGGA2450.022.04199816
GGTATCA4800.022.0388851
CCACGAG1700.021.17605620
AACGCAG13700.020.8572566
ACGCAGA13750.020.7814127
TCAACGC13900.020.719024
TACGGGG6550.020.61179415
GCCCACG1651.8189894E-1220.45564318
CGTTAAA550.003935374320.4541452
Produced by FastQC (version 0.11.2)