FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_11.34100000004869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_11.34100000004869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655915
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA250.002106292936.0005510
GCCGCGC250.002107081235.9978038
CCGCGCC250.002107081235.9978039
CGCTTAG300.00514720129.9981739
CCGATCA507.1567636E-526.9983529
CGGGGTA450.001225884325.00038116
TATGCCG751.6966951E-623.99853543
GGTATCA5800.022.5089241
GTATCAA10300.022.0718571
ACGCCTA1109.966061E-820.45485721
CGTGCAA550.003936154320.4532979
CGCCTAA1009.0414323E-720.25030922
CATCTCC21650.020.05957245
GCCCACG807.560222E-519.68629818
TCTCCGA5150.019.66199545
CTCCGAG3050.019.18208345
GAGGTCG600.006509877318.74885735
ACCGTGC600.006509877318.7488577
GTAAGTA3050.018.4414982
ATCTCCG8000.017.72037145
Produced by FastQC (version 0.11.2)