##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 6h_10.3410000000485d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51812 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.184223731953985 38.0 38.0 38.0 32.0 38.0 2 36.20684397436887 38.0 38.0 38.0 32.0 38.0 3 36.13757430711032 38.0 38.0 38.0 32.0 38.0 4 36.27397128078437 38.0 38.0 38.0 32.0 38.0 5 36.30778584111789 38.0 38.0 38.0 32.0 38.0 6 38.16144908515402 40.0 38.0 40.0 38.0 40.0 7 38.09304794256157 40.0 38.0 40.0 38.0 40.0 8 38.0391801127152 40.0 38.0 40.0 32.0 40.0 9 38.07025399521346 40.0 38.0 40.0 38.0 40.0 10 38.03763606886436 40.0 38.0 40.0 32.0 40.0 11 38.05265189531383 40.0 38.0 40.0 32.0 40.0 12 37.97029645641936 40.0 38.0 40.0 32.0 40.0 13 37.92700532695129 40.0 38.0 40.0 32.0 40.0 14 37.89651046089709 40.0 38.0 40.0 32.0 40.0 15 37.92138886744384 40.0 38.0 40.0 32.0 40.0 16 37.91922720605265 40.0 38.0 40.0 32.0 40.0 17 37.90008106230217 40.0 38.0 40.0 32.0 40.0 18 37.918725391801125 40.0 38.0 40.0 32.0 40.0 19 37.90799428703775 40.0 38.0 40.0 32.0 40.0 20 37.910098046784526 40.0 38.0 40.0 32.0 40.0 21 37.91042615610284 40.0 38.0 40.0 32.0 40.0 22 37.891009804678454 40.0 38.0 40.0 32.0 40.0 23 37.882421060758126 40.0 38.0 40.0 32.0 40.0 24 37.898189608584886 40.0 38.0 40.0 32.0 40.0 25 37.884679224889986 40.0 38.0 40.0 32.0 40.0 26 37.85119277387478 40.0 38.0 40.0 32.0 40.0 27 37.82897784297074 40.0 38.0 40.0 32.0 40.0 28 37.78873620010808 40.0 38.0 40.0 32.0 40.0 29 37.75457422990813 40.0 38.0 40.0 32.0 40.0 30 37.751408940013896 40.0 38.0 40.0 32.0 40.0 31 37.74880336601559 40.0 38.0 40.0 32.0 40.0 32 37.760248591059984 40.0 38.0 40.0 32.0 40.0 33 37.69534084768007 40.0 38.0 40.0 32.0 40.0 34 37.71498880568208 40.0 38.0 40.0 32.0 40.0 35 37.695977765768546 40.0 38.0 40.0 32.0 40.0 36 37.66928510769706 40.0 38.0 40.0 32.0 40.0 37 37.68157955685942 40.0 38.0 40.0 32.0 40.0 38 37.67316451787231 40.0 38.0 40.0 32.0 40.0 39 37.67046244113333 40.0 38.0 40.0 32.0 40.0 40 37.643827684706245 40.0 38.0 40.0 32.0 40.0 41 37.694954836717365 40.0 38.0 40.0 32.0 40.0 42 37.704605110785145 40.0 38.0 40.0 32.0 40.0 43 37.64533312746082 40.0 38.0 40.0 32.0 40.0 44 37.63128232841813 40.0 38.0 40.0 32.0 40.0 45 37.66581100903266 40.0 38.0 40.0 32.0 40.0 46 37.608160271751714 40.0 38.0 40.0 32.0 40.0 47 37.635682853393035 40.0 38.0 40.0 32.0 40.0 48 37.60532309117579 40.0 38.0 40.0 32.0 40.0 49 37.591195089940555 40.0 38.0 40.0 32.0 40.0 50 37.61246429398595 40.0 38.0 40.0 32.0 40.0 51 37.0429244190535 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.3554375392489817 2201 2 -0.4338125542799034 2201 3 -0.46209974269593346 2201 4 -0.06829111930328224 2201 5 -0.7457076763277541 2201 6 -0.4464236881237653 2201 7 0.23797360757440345 2201 8 0.2547789373068312 2201 9 0.6381712428742148 2201 10 0.37265113794985893 2201 11 0.5803094373550479 2201 12 0.44377013999059045 2201 13 -0.2740806479654907 2201 14 -0.36844316169387525 2201 15 -0.28320663119924205 2201 16 0.13179427134813437 2201 17 -0.17681443498338467 2201 18 0.13675440154271 2201 19 0.6203390127713533 2201 20 -1.650158104618626 2201 21 -0.14586077250580587 2201 22 -0.24557175774188522 2201 23 -0.45909810399230366 2201 24 -0.39404542637183226 2201 25 0.1400678732223355 2201 26 -0.20987208454273087 2201 27 -0.8738877416706643 2201 28 0.6406244612952676 2201 29 -0.14824603475467768 2201 30 -0.023158962378801107 2201 31 -0.2980092984655869 2201 32 0.19987971198151655 2201 33 0.2330047670664186 2201 34 0.2425654484645321 2201 35 -0.017263367293125498 2201 36 0.07800844201814527 2201 37 0.6047392700681087 2201 38 0.27130024109666095 2201 39 0.3469426448338382 2201 40 -0.2021372944327524 2201 41 -0.8869816749540362 2201 42 -0.9629770873288024 2201 43 -1.428647813556772 2201 44 0.15128175807652156 2201 45 -0.3753930849950038 2201 46 0.08642115382272664 2201 47 0.7102917885969617 2201 48 0.4995210169528761 2201 49 0.2822954222794607 2201 50 0.15040342053195133 2201 51 -0.17320273062324532 1101 1 0.49007235420611295 1101 2 0.18700175317823664 1101 3 0.11647712183983572 1101 4 -0.39972186511759134 1101 5 0.052998564159310035 1101 6 0.2712323240527894 1101 7 0.1034606699792775 1101 8 -0.2562560703035217 1101 9 0.2443356264358556 1101 10 0.0996298289696469 1101 11 0.005537747857331965 1101 12 0.03262097712909906 1101 13 0.5680045879553575 1101 14 0.2506588169971451 1101 15 0.2963215270960404 1101 16 0.9655081221852768 1101 17 0.49898465177460594 1101 18 0.0941364411165253 1101 19 0.028824553106204576 1101 20 -0.5365736297327857 1101 21 0.5735075684378756 1101 22 -0.6534865218210371 1101 23 -0.03634315726475279 1101 24 -0.7349891097812673 1101 25 0.3900678732223355 1101 26 -0.21577010585171053 1101 27 0.15255822484379422 1101 28 0.8409669270486901 1101 29 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0.42107923741756537 2202 15 0.4772524580269675 2202 16 0.7530456597228081 2202 17 -0.23975408696413325 2202 18 0.6917340387141238 2202 19 0.6147855140671652 2202 20 1.17175970360055 2202 21 0.6406997606300706 2202 22 0.7323993640648467 2202 23 0.4944376235160277 2202 24 0.3836295088373447 2202 25 0.7752030083574724 2202 26 1.1066773415957414 2202 27 1.235516182801753 2202 28 0.9592101702919322 2202 29 0.45153182529715963 2202 30 0.33133937157158755 2202 31 0.2649303535151617 2202 32 0.39017960091153725 2202 33 0.8433342746562005 2202 34 0.32549769578034216 2202 35 1.1911779507372344 2202 36 1.0015552765238809 2202 37 0.35779369435540787 2202 38 0.11061901192227452 2202 39 0.3462021783399507 2202 40 -0.10050826814619285 2202 41 0.6968761554791385 2202 42 1.2036278737967052 2202 43 0.6502118680426321 2202 44 0.7760503622971839 2202 45 0.837120798751755 2202 46 0.11937191280088655 2202 47 0.4228056723437206 2202 48 0.16057247937420271 2202 49 0.09588298244236171 2202 50 0.6681746163853362 2202 51 1.0185780912945646 1102 1 -0.18086781673405739 1102 2 -0.2991093579328705 1102 3 0.39318652354923245 1102 4 0.15369693830120923 1102 5 -0.188454427293685 1102 6 0.4079844608049328 1102 7 0.06393075544936266 1102 8 0.6903251262776777 1102 9 0.1481817802820089 1102 10 0.7224930768328974 1102 11 -0.44638532906574113 1102 12 0.5849714044795249 1102 13 -0.03242276247199527 1102 14 0.09467591101424233 1102 15 0.09333007410458549 1102 16 -0.33150042482327535 1102 17 0.37077952356947463 1102 18 0.4616578086378951 1102 19 0.3119442112258639 1102 20 0.7358622677031121 1102 21 0.14936226929258112 1102 22 0.3294194610849459 1102 23 0.14100727008567304 1102 24 0.948771573979414 1102 25 0.3451960783505399 1102 26 0.1635033984217955 1102 27 0.5809770282625948 1102 28 0.6262233373050989 1102 29 0.6455720193373509 1102 30 0.4917690320012511 1102 31 0.11593520452001371 1102 32 0.7051484158803518 1102 33 0.26918320050512534 1102 34 0.3795517498343983 1102 35 0.7022659618252476 1102 36 1.0999613749299826 1102 37 1.0358948724565877 1102 38 0.48345338475665045 1102 39 -0.09246726032949226 1102 40 0.587641420003493 1102 41 0.21593367453665735 1102 42 -0.1470304768616444 1102 43 0.3428660606968279 1102 44 0.6277482139950337 1102 45 0.10704456867552636 1102 46 0.34529013871910763 1102 47 0.4363191858572364 1102 48 0.4557907745925007 1102 49 0.6672620538214389 1102 50 0.9398308880416053 1102 51 0.18524475796122886 2203 1 0.17810654224030031 2203 2 0.22653166770815147 2203 3 -0.35040322004051205 2203 4 -0.05143126682699517 2203 5 0.6192378803986216 2203 6 0.523369076189546 2203 7 0.7946999862185891 2203 8 0.6390430749956266 2203 9 -0.0825874504872246 2203 10 0.5686469229867441 2203 11 0.5151531324727188 2203 12 0.8413816608897804 2203 13 0.5316798016305739 2203 14 0.4280092443475709 2203 15 0.5548685356430454 2203 16 0.6043970110741625 2203 17 0.44770260049255484 2203 18 0.7052475522276396 2203 19 1.2478416471233018 2203 20 0.8768879087287544 2203 21 0.8416699616002745 2203 22 1.0345476662131503 2203 23 1.089725218803622 2203 24 0.7436433688512096 2203 25 0.5887858219402844 2203 26 0.4840162189346131 2203 27 0.6450795923651569 2203 28 0.33135154243330334 2203 29 0.7865976603629932 2203 30 0.5558715961038132 2203 31 0.3979864865712983 2203 32 0.15386636459830072 2203 33 0.9230293543512857 2203 34 0.9949363652190115 2203 35 0.2535480131072987 2203 36 1.0999613749299826 2203 37 0.689741026302741 2203 38 0.35524825655151915 2203 39 0.7536865858243544 2203 40 0.6773850097470842 2203 41 0.43388239248537275 2203 42 0.570918241087071 2203 43 0.8428660606968279 2203 44 0.26877385502067597 2203 45 0.23524969688065767 2203 46 0.6017003951293702 2203 47 0.7055499550880029 2203 48 0.609636928438654 2203 49 0.6288005153598988 2203 50 0.4398308880416053 2203 51 0.8647319374484113 1103 1 0.5389344693332916 1103 2 0.4097259834325868 1103 3 -0.05939302541585789 1103 4 -0.21732561344423118 1103 5 -0.1682196419986539 1103 6 -0.587536082908386 1103 7 -0.16143223497387282 1103 8 -0.5892547316154335 1103 9 0.11481755414669692 1103 10 -0.05415196487238916 1103 11 -0.46986385856836677 1103 12 0.11879902264448816 1103 13 -0.5789164294475881 1103 14 0.03211798701053681 1103 15 -0.539354510387227 1103 16 0.020059661676569362 1103 17 0.20179589675452547 1103 18 0.458259600420412 1103 19 -0.14001748169968664 1103 20 -0.0692041518211397 1103 21 0.7343174747297141 1103 22 -0.5865521175372379 1103 23 -0.017936196086708378 1103 24 -0.5689917871574437 1103 25 -0.8960767050909197 1103 26 -0.15870852619976716 1103 27 -0.7900455234828456 1103 28 0.05934042102459358 1103 29 -0.06588921019616834 1103 30 0.5229707619981596 1103 31 0.11856109268806847 1103 32 0.24592691449017678 1103 33 -0.8556237194825158 1103 34 -0.21080969594873267 1103 35 0.19052669707393477 1103 36 -0.6759113467258757 1103 37 0.4753449805813972 1103 38 -0.2576649346749207 1103 39 -0.033153080533381285 1103 40 -0.10327609621522527 1103 41 -0.6212612590438198 1103 42 -0.694450927896554 1103 43 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1213 39 -0.5788196176495859 1213 40 -1.638578596539098 1213 41 -1.1653731906411764 1213 42 -1.6127045876970456 1213 43 -1.0553969665984582 1213 44 -1.0674544328205187 1213 45 -0.9396882683798893 1213 46 -1.33543773555715 1213 47 -1.0798098464008277 1213 48 -0.9912729144066716 1213 49 -1.0068487815797198 1213 50 -1.1366786239517737 1213 51 -0.5943251345118838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 5.0 21 12.0 22 18.0 23 52.0 24 74.0 25 128.0 26 195.0 27 334.0 28 465.0 29 624.0 30 735.0 31 967.0 32 1263.0 33 1460.0 34 1770.0 35 2240.0 36 2922.0 37 4592.0 38 8474.0 39 25481.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.68762552139657 19.787965394716515 14.004325660435658 27.520083423451258 2 14.98137461156897 23.240238559379282 38.698345911099956 23.080040917951784 3 17.598239790010037 26.167683162201804 31.035281401991817 25.19879564579634 4 13.834632903574462 17.910908669806222 37.77696286574539 30.47749556087393 5 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18 22.09268659885348 28.500839622459416 28.110945974637612 21.295527804049488 19 22.93098123986721 27.943333590673973 27.692426464911602 21.43325870454721 20 22.948351733189224 28.13247896240253 27.470470161352583 21.448699143055663 21 22.31143364471551 27.456959777657687 27.82946035667413 22.402146220952677 22 22.25353200030881 27.958774029182425 27.586273450165983 22.201420520342776 23 22.353894850613756 27.93368331660619 27.16166139118351 22.55076044159654 24 21.91770246274994 28.144059291283874 27.94719370030109 21.991044545665098 25 22.02964564193623 27.85841117887748 27.55925268277619 22.5526904964101 26 21.95437350420752 27.74260789006408 28.05141666023315 22.251601945495253 27 22.216860958851232 28.445147842198722 27.49363081911526 21.844360379834786 28 22.081757121902264 27.7194472323014 27.94719370030109 22.251601945495253 29 22.334594302478187 28.12089863352119 27.48977070948815 22.054736354512467 30 22.076393043948197 28.223736272220183 27.521182760417673 22.178687923413946 31 22.566200880104994 26.677217632980778 27.23500347409866 23.521578012815564 32 21.62047402146221 28.04562649579248 28.034046166911143 22.29985331583417 33 22.496718906816955 26.903034046166912 27.87771172701305 22.722535320003086 34 22.272832548444377 26.08469080521887 28.589901953215467 23.052574693121283 35 22.243881726241025 26.86250289508222 28.026325947656915 22.86728943101984 36 22.06052651895314 25.573226279626343 28.858179572299854 23.508067629120667 37 21.75557785841118 25.428472168609588 28.477958774029183 24.33799119895005 38 21.541341774106385 24.639079749864894 29.562649579248053 24.256928896780668 39 21.066548289971436 24.179726704238398 30.089554543349028 24.664170462441135 40 21.246043387632206 23.897938701459122 30.985099976839344 23.87091793406933 41 21.361846676445612 23.351733189222575 31.06230216938161 24.224117964950203 42 20.48560179109087 23.87091793406933 31.13950436192388 24.503975912915926 43 19.62286728943102 23.68756272678144 31.276538253686404 25.41303173010113 44 19.244576545973903 24.181656759051958 31.521655215008103 25.052111479966033 45 18.70223114336447 23.556318999459585 32.63529684243032 25.10615301474562 46 18.61344862194086 24.218327800509535 31.768702231143365 25.39952134640624 47 18.003551300856945 24.57345788620397 32.28016675673589 25.142824056203196 48 17.328032116112098 25.237396742067475 31.8516945881263 25.582876553694124 49 17.43804524048483 24.803134409017215 31.89608584883811 25.862734501659844 50 16.415116189299773 25.237396742067475 31.63938855863507 26.708098509997686 51 15.928656912326758 25.193992973786823 30.498397868972702 28.37895224491372 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 1.0 3 1.5 4 2.0 5 2.5 6 3.0 7 3.5 8 4.0 9 5.0 10 6.0 11 6.0 12 6.0 13 12.5 14 19.0 15 20.5 16 22.0 17 24.0 18 26.0 19 21.0 20 16.0 21 16.5 22 17.0 23 24.0 24 31.0 25 32.0 26 50.5 27 68.0 28 150.0 29 232.0 30 427.0 31 622.0 32 1186.5 33 1751.0 34 2637.5 35 3524.0 36 4510.0 37 5496.0 38 6072.0 39 6648.0 40 6493.5 41 6339.0 42 5649.5 43 4960.0 44 4347.5 45 3735.0 46 3403.0 47 3071.0 48 2832.0 49 2593.0 50 2432.5 51 2272.0 52 2094.0 53 1916.0 54 1813.0 55 1710.0 56 1593.5 57 1477.0 58 1391.0 59 1305.0 60 1221.5 61 1138.0 62 1007.0 63 876.0 64 784.0 65 692.0 66 563.0 67 434.0 68 365.0 69 296.0 70 251.0 71 206.0 72 165.0 73 124.0 74 97.5 75 52.0 76 33.0 77 28.5 78 24.0 79 16.0 80 8.0 81 5.0 82 2.0 83 1.5 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.054041534779587744 2 0.0019300548135567051 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0019300548135567051 17 0.0 18 0.005790164440670115 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0019300548135567051 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.01158032888134023 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 51812.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.178381983879 #Duplication Level Percentage of deduplicated Percentage of total 1 95.24482495228979 88.74758681392149 2 3.6471449272716283 6.796701263677648 3 0.6194530080549394 1.7315888701681812 4 0.22164263919952548 0.8260920999699386 5 0.0829179117514974 0.3863078427243298 6 0.05813560253205476 0.32501888277568575 7 0.041569471752184346 0.27113632824551714 8 0.019462006505964666 0.14507506211084115 9 0.013193925136951935 0.11064497366498494 >10 0.05165555550547411 0.6598478627413622 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.001930054813556705 20 0.0 0.0 0.001930054813556705 21 0.0 0.0 0.001930054813556705 22 0.0 0.0 0.00386010962711341 23 0.0 0.0 0.021230602949123756 24 0.0 0.0 0.036671041457577394 25 0.0 0.0 0.040531151084690804 26 0.0 0.0 0.050181425152474333 27 0.0 0.0 0.06562186366092797 28 0.0 0.0 0.13317378213541264 29 0.0 0.0 0.2277464679996912 30 0.0 0.0 0.47672353894850616 31 0.0 0.0 1.3510383694896935 32 0.0 0.0 2.34887670809851 33 0.0 0.0 3.402686636300471 34 0.0 0.0 4.67459275843434 35 0.0 0.0 5.988960086466456 36 0.0 0.0 7.457731799583108 37 0.0 0.0 9.434107928665174 38 0.0 0.0 11.709642553848528 39 0.0 0.0 14.07588975526905 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGAACC 20 6.971014E-4 45.0 4 TATCTCG 20 6.971014E-4 45.0 36 CTCGTAT 30 1.1249434E-4 37.499996 39 TGCCGTC 30 1.1249434E-4 37.499996 45 GTATTGG 35 2.783603E-4 32.142857 1 TATGCCG 35 2.783603E-4 32.142857 43 CCCACGA 35 2.783603E-4 32.142857 19 TATTGGA 35 2.783603E-4 32.142857 2 TCTCGTA 35 2.783603E-4 32.142857 38 CCACGAG 35 2.783603E-4 32.142857 20 AGACAGG 30 0.0051025366 29.999998 6 CACGAGA 40 6.086366E-4 28.125 21 GAGCCCA 40 6.086366E-4 28.125 16 ACGAGAC 40 6.086366E-4 28.125 22 ATCTCGT 45 0.0012108085 25.0 37 TCGTATG 45 0.0012108085 25.0 40 CGAGCCC 45 0.0012108085 25.0 15 TGAGGAC 45 0.0012108085 25.0 3 GCCCACG 50 0.0022357644 22.5 18 AGCCCAC 50 0.0022357644 22.5 17 >>END_MODULE