FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_10.3410000000485d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_10.3410000000485d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51812
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAACC206.971014E-445.04
TATCTCG206.971014E-445.036
CTCGTAT301.1249434E-437.49999639
TGCCGTC301.1249434E-437.49999645
GTATTGG352.783603E-432.1428571
TATGCCG352.783603E-432.14285743
CCCACGA352.783603E-432.14285719
TATTGGA352.783603E-432.1428572
TCTCGTA352.783603E-432.14285738
CCACGAG352.783603E-432.14285720
AGACAGG300.005102536629.9999986
CACGAGA406.086366E-428.12521
GAGCCCA406.086366E-428.12516
ACGAGAC406.086366E-428.12522
ATCTCGT450.001210808525.037
TCGTATG450.001210808525.040
CGAGCCC450.001210808525.015
TGAGGAC450.001210808525.03
GCCCACG500.002235764422.518
AGCCCAC500.002235764422.517
Produced by FastQC (version 0.11.2)