FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 6h_1.34100000004727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 6h_1.34100000004727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241985
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAA250.002103876835.99983614
TACGACC250.002103876835.99983633
GGTTTAC300.0051394429.9998629
CGACAAT300.0051394429.9998619
TTACGAC300.0051394429.9998632
TCGGATA406.1521324E-428.12487213
TTACTAG651.5173544E-524.23065810
GTCCTAC500.00225683222.5045471
CCTACAC500.002259545322.4998973
GAAAGCG500.002259545322.4998979
GCTAGTC500.002259545322.4998977
TGCAGGA602.459784E-422.4998952
GGTATCA1056.0448656E-821.4329011
TCCTACA955.4948396E-721.3156912
AGGCTTA754.5835357E-520.9999036
GTATCAA2050.020.8578741
AACCATG550.00392780320.4544537
CTACACT550.00392780320.4544534
CTGATTA1008.9948117E-720.2499089
AGGGTGA908.207457E-619.99990718
Produced by FastQC (version 0.11.2)