##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_96.34100000006e1c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 46972 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.29409009622754 38.0 38.0 38.0 32.0 38.0 2 36.30499020693179 38.0 38.0 38.0 32.0 38.0 3 36.27084220386613 38.0 38.0 38.0 32.0 38.0 4 36.40424082432087 38.0 38.0 38.0 32.0 38.0 5 36.419164608703056 38.0 38.0 38.0 32.0 38.0 6 38.35327429106702 40.0 38.0 40.0 38.0 40.0 7 38.23379885889466 40.0 38.0 40.0 38.0 40.0 8 38.18674955292515 40.0 38.0 40.0 38.0 40.0 9 38.24018564251043 40.0 38.0 40.0 38.0 40.0 10 38.22087626671208 40.0 38.0 40.0 38.0 40.0 11 38.170676147492124 40.0 38.0 40.0 38.0 40.0 12 38.09137358426297 40.0 38.0 40.0 38.0 40.0 13 38.11164097760368 40.0 38.0 40.0 38.0 40.0 14 38.07468278974709 40.0 38.0 40.0 38.0 40.0 15 38.02533424167589 40.0 38.0 40.0 32.0 40.0 16 38.0793451417866 40.0 38.0 40.0 38.0 40.0 17 38.047326066592866 40.0 38.0 40.0 32.0 40.0 18 38.069679809248065 40.0 38.0 40.0 38.0 40.0 19 38.09765392148514 40.0 38.0 40.0 38.0 40.0 20 38.08128246615005 40.0 38.0 40.0 38.0 40.0 21 38.10736183258111 40.0 38.0 40.0 38.0 40.0 22 38.082474665758326 40.0 38.0 40.0 38.0 40.0 23 38.08494422208975 40.0 38.0 40.0 38.0 40.0 24 38.06821084901644 40.0 38.0 40.0 32.0 40.0 25 38.054606999914846 40.0 38.0 40.0 32.0 40.0 26 37.972749723239374 40.0 38.0 40.0 32.0 40.0 27 37.95171591586477 40.0 38.0 40.0 32.0 40.0 28 37.90526270969939 40.0 38.0 40.0 32.0 40.0 29 37.914012603253 40.0 38.0 40.0 32.0 40.0 30 37.90413437792728 40.0 38.0 40.0 32.0 40.0 31 37.90306991399132 40.0 38.0 40.0 32.0 40.0 32 37.867048454398365 40.0 38.0 40.0 32.0 40.0 33 37.81814698118028 40.0 38.0 40.0 32.0 40.0 34 37.80960998041386 40.0 38.0 40.0 32.0 40.0 35 37.82610917142127 40.0 38.0 40.0 32.0 40.0 36 37.749382610917145 40.0 38.0 40.0 32.0 40.0 37 37.75647194073065 40.0 38.0 40.0 32.0 40.0 38 37.73748190411309 40.0 38.0 40.0 32.0 40.0 39 37.69564847142979 40.0 38.0 40.0 32.0 40.0 40 37.655603338158905 40.0 38.0 40.0 32.0 40.0 41 37.600868602571744 40.0 38.0 40.0 32.0 40.0 42 37.58462488290897 40.0 38.0 40.0 32.0 40.0 43 37.512390360214596 40.0 38.0 40.0 32.0 40.0 44 37.45450481137699 40.0 38.0 40.0 32.0 40.0 45 37.4040492208124 40.0 38.0 40.0 32.0 40.0 46 37.25804734735587 40.0 38.0 40.0 32.0 40.0 47 37.24476283743507 40.0 38.0 40.0 32.0 40.0 48 37.13884867580686 40.0 38.0 40.0 32.0 40.0 49 37.09060717022907 40.0 38.0 40.0 32.0 40.0 50 37.07983479519714 40.0 38.0 40.0 32.0 40.0 51 36.60020863493145 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.5390243149212495 2201 2 0.6500151703222841 2201 3 0.04687314072248938 2201 4 -0.14521248800001985 2201 5 0.2436182721673248 2201 6 0.5310318603327673 2201 7 0.4331714836947782 2201 8 -0.15721711203401867 2201 9 0.12708409766555206 2201 10 -1.0517584437325667 2201 11 -0.17690132155991023 2201 12 -0.37748927999257376 2201 13 0.1780662350764075 2201 14 -0.21629333201445178 2201 15 -0.28562430496065616 2201 16 -0.5211661959928549 2201 17 -0.4732071306689676 2201 18 -0.5171919866955363 2201 19 0.1479204358633055 2201 20 0.5240328450404093 2201 21 -0.33385811523454123 2201 22 -0.8469052350227955 2201 23 -1.2189706332837744 2201 24 0.26665279295323074 2201 25 0.043772504263564826 2201 26 0.15809473452193856 2201 27 -0.4257696855853439 2201 28 0.5004715205502066 2201 29 -1.0456619074680376 2201 30 -0.046244705694945765 2201 31 0.6061416673889752 2201 32 -0.01190071865942599 2201 33 0.5414287756254055 2201 34 -0.7904059480855707 2201 35 -0.41194409465941106 2201 36 -0.08187917545875223 2201 37 0.863202520797735 2201 38 0.7784969147391507 2201 39 0.7081416365666939 2201 40 0.13132180642577396 2201 41 0.44970656614754745 2201 42 0.4297987461149617 2201 43 0.10118183756379295 2201 44 0.07031592738546522 2201 45 0.6532724434376362 2201 46 0.7486899075211397 2201 47 0.5350704044962669 2201 48 0.9529856353620616 2201 49 0.9951402700885268 2201 50 -0.044034500887811134 2201 51 0.196272797874677 1101 1 0.24961517333819927 1101 2 -0.8648120314614403 1101 3 -0.4692918537033748 1101 4 0.45278082303676825 1101 5 0.07015115956978235 1101 6 -0.2571509713283291 1101 7 0.24610347923546527 1101 8 -0.012512541242493569 1101 9 -0.12843429698327924 1101 10 0.061285034528303584 1101 11 0.3562090463330705 1101 12 -0.37637445279079174 1101 13 -0.8845870536638358 1101 14 -0.08496668764432513 1101 15 -0.10256967842776987 1101 16 -0.12072026511214062 1101 17 0.30182074001107395 1101 18 0.1755616364928727 1101 19 0.18025042471503383 1101 20 0.7153371928664924 1101 21 0.14863909769745476 1101 22 -0.19919063078645394 1101 23 0.26887775021678806 1101 24 -0.40068277003450703 1101 25 -0.5653690787910648 1101 26 0.16968893742048863 1101 27 0.11670523080261574 1101 28 -0.6618473200295014 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1213 4 -1.2021099651873541 1213 5 -0.7955658394805525 1213 6 -0.7690218165325078 1213 7 -0.8201833203846647 1213 8 -0.20928367134695947 1213 9 -0.17135927324159184 1213 10 -0.10167792843466117 1213 11 -0.7195744294504038 1213 12 -0.5406669504166217 1213 13 -0.7328301685736207 1213 14 -1.0756599450042543 1213 15 -1.18310149229292 1213 16 -0.5098940542859296 1213 17 -0.9192618810715487 1213 18 -0.5182655240009595 1213 19 -0.7694171916192474 1213 20 0.12521373607636832 1213 21 -1.013943998218977 1213 22 0.21106577946996197 1213 23 -0.5168772355382032 1213 24 -0.7811196171380175 1213 25 0.3744219943333462 1213 26 0.253639554704435 1213 27 -0.4230858423217967 1213 28 -0.20752633237518125 1213 29 0.132008516579198 1213 30 -0.6152194775682673 1213 31 -0.8640676723855805 1213 32 0.3289473758118575 1213 33 0.3476552919968512 1213 34 -0.16077631845594453 1213 35 0.04216218553382589 1213 36 0.3169399335052816 1213 37 -0.3155414405549237 1213 38 -0.12434795912021457 1213 39 -1.518911503529928 1213 40 -0.003407125404606859 1213 41 -1.1240991236001747 1213 42 0.24890771015146385 1213 43 -0.3459464501442042 1213 44 0.07138946469088836 1213 45 -0.023592827630537272 1213 46 -0.5411651649426332 1213 47 -0.19976588106465698 1213 48 -1.1382650355987565 1213 49 -0.5690035839103942 1213 50 -0.5008246243446024 1213 51 -0.8209037990120649 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 0.0 20 8.0 21 10.0 22 17.0 23 28.0 24 74.0 25 136.0 26 182.0 27 327.0 28 396.0 29 523.0 30 679.0 31 874.0 32 1053.0 33 1295.0 34 1624.0 35 2002.0 36 2670.0 37 4087.0 38 7273.0 39 23712.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.33436268767969 18.97987434777979 12.424661910339687 28.261101054200832 2 14.977964189146032 21.094764631991314 39.58781323851902 24.33945794034363 3 19.811802776121944 23.15209060717023 29.59848420335519 27.437622413352635 4 13.772034403474409 18.70901813846547 33.96065741292685 33.55829004513327 5 14.925913310057053 35.425359788810354 34.269351954355784 15.379374946776803 6 32.30648045644214 37.32862130631015 15.979732606659287 14.385165630588437 7 27.769735161372733 31.850889891850464 19.586136421698033 20.79323852507877 8 24.72536830452184 36.46853444605297 19.560589287234947 19.245507962190242 9 24.469896959891 17.663714553350932 19.939538448437368 37.9268500383207 10 16.284169292344373 29.817763774163332 30.499020693178913 23.39904624031338 11 32.95650507760107 23.242000383215174 24.968597645355644 18.832896893828106 12 23.643872945584604 24.7125947372903 28.450992080388314 23.192540236736782 13 27.835731925402367 22.07485310397684 27.22260069828834 22.86681427233245 14 20.99548667291152 21.740611428084815 26.65630588435664 30.607596014647026 15 22.728433960657412 29.34301285872435 23.609810099633822 24.31874308098442 16 24.02282210678702 28.38286638848676 25.46836413182321 22.125947372903006 17 20.857106361236482 29.934854807119134 27.29711317380567 21.91092565783871 18 22.992421016775953 26.30716171336115 29.411138550625903 21.289278719236993 19 21.827897470833687 26.47960487098697 28.461636719747936 23.230860938431405 20 21.97692242186835 26.211359959124586 28.65962701183684 23.15209060717023 21 21.280763007749297 26.705271225410883 28.01669079451588 23.997274972323936 22 21.751256067444437 28.9704504811377 27.997530443668566 21.280763007749297 23 21.280763007749297 26.807459763263218 28.361577109767524 23.550200119219962 24 23.403304096057227 26.24755173294729 29.16631184535468 21.182832325640806 25 21.159414119049647 28.440347441028695 28.957676913906155 21.4425615260155 26 22.987012987012985 26.69150521609538 28.986587183308494 21.33489461358314 27 21.063612364813082 29.13012007153198 28.559567401856423 21.24670016179852 28 20.969939538448436 26.424252746316956 28.461636719747936 24.14417099548667 29 21.715064293621733 26.600953759686625 28.212552158732862 23.471429787958783 30 21.265860512645833 26.639274461381245 28.576598824831816 23.518266201141106 31 22.059950608873372 26.275227795282298 29.926339095631437 21.738482500212893 32 23.456527292855316 26.947969002810186 28.34241675892021 21.25308694541429 33 22.783786085327428 27.748445882653495 28.412671378693688 21.055096653325386 34 21.34675977177893 28.86187515966959 28.789491612024186 21.001873456527292 35 21.09128842714809 27.371625649323 30.098782253257262 21.43830367027165 36 20.910329558034572 29.127991143660054 28.82142553010304 21.14025376820233 37 21.257344801158137 28.529762411649497 28.006046155156263 22.206846632036108 38 20.86136421698033 27.103380737460615 30.32444860768117 21.710806437877885 39 21.527718640892445 26.33696670356808 28.540407051009115 23.594907604530356 40 22.360129438814614 25.755769394532912 30.62675636549434 21.257344801158137 41 21.94924635953334 25.781316528995994 28.199778591501317 24.069658519969344 42 23.460785148599165 26.809588691135144 28.408413522949843 21.32121263731585 43 20.882653495699564 26.573277697351617 30.675721706548583 21.868347100400236 44 20.56331431491101 28.67240057906838 28.778846972664567 21.98543813335604 45 20.872008856339946 26.594566976070848 30.43302392914928 22.10040023843992 46 23.196798092480627 26.060206080218002 27.933662607510858 22.809333219790513 47 21.574555054074768 26.419994890573108 27.646257344801157 24.359192710550968 48 20.761304606999914 26.55624627437622 27.68032019075194 25.002128927871926 49 23.648130801328453 26.35399812654347 27.10976752107638 22.888103551051692 50 20.35042152771864 27.13744358341139 29.888018393936814 22.62411649493315 51 20.829430298901475 26.747849782849357 27.571744869283826 24.85097504896534 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 243.0 1 141.0 2 39.0 3 46.0 4 53.0 5 56.5 6 60.0 7 52.5 8 45.0 9 63.5 10 82.0 11 132.0 12 182.0 13 275.5 14 369.0 15 441.5 16 514.0 17 467.0 18 420.0 19 359.0 20 298.0 21 332.5 22 367.0 23 328.0 24 289.0 25 309.5 26 383.5 27 437.0 28 490.0 29 543.0 30 713.0 31 883.0 32 1178.0 33 1473.0 34 1783.5 35 2094.0 36 2341.5 37 2589.0 38 2645.0 39 2701.0 40 2739.0 41 2777.0 42 2858.5 43 2940.0 44 3002.0 45 3064.0 46 3091.5 47 3119.0 48 3121.5 49 3124.0 50 3187.5 51 3251.0 52 3542.0 53 3833.0 54 3189.0 55 2545.0 56 2357.0 57 2169.0 58 1997.0 59 1825.0 60 1571.0 61 1317.0 62 1178.5 63 1040.0 64 875.0 65 710.0 66 602.0 67 494.0 68 397.5 69 301.0 70 224.0 71 147.0 72 144.0 73 141.0 74 109.5 75 64.0 76 50.0 77 35.0 78 20.0 79 16.0 80 12.0 81 7.0 82 2.0 83 1.5 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03619177382270289 2 0.006386783615771097 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.002128927871923699 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.004257855743847398 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 46972.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.69355368007616 #Duplication Level Percentage of deduplicated Percentage of total 1 92.78511671416163 80.43871496571305 2 5.660870506993878 9.815219623480674 3 0.8995539818534759 2.339565942418217 4 0.30661856866362985 1.0632741336699405 5 0.10847632405724399 0.470209901133701 6 0.052603968044520145 0.2736254956471571 7 0.05207820919097432 0.3160391516841977 8 0.009223149370454216 0.06396700760374667 9 0.023529107331814243 0.18358397365634208 >10 0.08221286925754899 1.1385914884923587 >50 0.0073937286978046435 0.4615117856785371 >100 0.009858297901719808 1.5020489186574548 >500 0.0024645744753374102 1.9336476121646944 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC 905 1.926679724090948 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 239 0.5088137613897642 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 232 0.4939112662862982 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 119 0.25334241675892016 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 113 0.24056884952737803 No Hit TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT 77 0.16392744613812482 No Hit CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 73 0.15541173465043004 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 66 0.14050923954696415 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.002128927871923699 16 0.0 0.0 0.002128927871923699 17 0.0 0.0 0.002128927871923699 18 0.0 0.0 0.002128927871923699 19 0.0 0.0 0.002128927871923699 20 0.0 0.0 0.004257855743847398 21 0.0 0.0 0.004257855743847398 22 0.0 0.0 0.004257855743847398 23 0.0 0.0 0.008515711487694796 24 0.0 0.0 0.012773567231542195 25 0.0 0.0 0.014902495103465894 26 0.0 0.0 0.02341820659116069 27 0.0 0.0 0.034062845950779186 28 0.0 0.0 0.04257855743847398 29 0.0 0.0 0.06386783615771098 30 0.0 0.0 0.08728604274887167 31 0.0 0.0 0.21927957080814103 32 0.0 0.0 0.3597888103551052 33 0.0 0.0 0.5386187515966959 34 0.0 0.0 0.7174486928382866 35 0.0 0.0 0.9388571915183513 36 0.0 0.0 1.1985863918930426 37 0.0 0.0 1.537085923528911 38 0.0 0.0 1.975645065145193 39 0.0 0.0 2.3588520820914587 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGT 115 0.0 43.039406 37 GCCCACG 115 0.0 43.039406 18 CGTATGC 115 0.0 43.039406 41 CTCGTAT 115 0.0 43.039406 39 AGCCCAC 115 0.0 43.039406 17 CACGAGA 115 0.0 43.039406 21 TATGCCG 115 0.0 43.039406 43 TCGTATG 115 0.0 43.039406 40 CGAGCCC 115 0.0 43.039406 15 TCTCCGA 115 0.0 43.039406 11 TGCCGTC 115 0.0 43.039406 45 GATAATC 110 0.0 42.950478 33 ATCTCCG 120 0.0 41.246094 10 CTCCGAG 120 0.0 41.246094 12 CCCACGA 120 0.0 41.246094 19 TCTCGTA 120 0.0 41.246094 38 GAGCCCA 120 0.0 41.246094 16 ACGAGAC 120 0.0 41.246094 22 AATCTCG 115 0.0 41.08307 36 GTATGCC 125 0.0 39.596252 42 >>END_MODULE