FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_96.34100000006e1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_96.34100000006e1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46972
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC9051.926679724090948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.5088137613897642No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.4939112662862982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.25334241675892016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.24056884952737803No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT770.16392744613812482No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC730.15541173465043004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.14050923954696415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT1150.043.03940637
GCCCACG1150.043.03940618
CGTATGC1150.043.03940641
CTCGTAT1150.043.03940639
AGCCCAC1150.043.03940617
CACGAGA1150.043.03940621
TATGCCG1150.043.03940643
TCGTATG1150.043.03940640
CGAGCCC1150.043.03940615
TCTCCGA1150.043.03940611
TGCCGTC1150.043.03940645
GATAATC1100.042.95047833
ATCTCCG1200.041.24609410
CTCCGAG1200.041.24609412
CCCACGA1200.041.24609419
TCTCGTA1200.041.24609438
GAGCCCA1200.041.24609416
ACGAGAC1200.041.24609422
AATCTCG1150.041.0830736
GTATGCC1250.039.59625242