##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_94.34100000006df1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45819 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.26842576223837 38.0 38.0 38.0 32.0 38.0 2 36.215107269909865 38.0 38.0 38.0 32.0 38.0 3 36.201379340448284 38.0 38.0 38.0 32.0 38.0 4 36.33134725768786 38.0 38.0 38.0 32.0 38.0 5 36.321504179488855 38.0 38.0 38.0 32.0 38.0 6 38.21281564416508 40.0 38.0 40.0 38.0 40.0 7 38.09334555533731 40.0 38.0 40.0 38.0 40.0 8 38.074292324145006 40.0 38.0 40.0 38.0 40.0 9 38.098976407167335 40.0 38.0 40.0 38.0 40.0 10 38.06951264759161 40.0 38.0 40.0 38.0 40.0 11 37.9885636962832 40.0 38.0 40.0 32.0 40.0 12 37.926318776053606 40.0 38.0 40.0 32.0 40.0 13 37.98498439511993 40.0 38.0 40.0 32.0 40.0 14 37.931032977585716 40.0 38.0 40.0 32.0 40.0 15 37.941880006111 40.0 38.0 40.0 32.0 40.0 16 37.884261987385145 40.0 38.0 40.0 32.0 40.0 17 37.89061306444925 40.0 38.0 40.0 32.0 40.0 18 37.941050655841465 40.0 38.0 40.0 32.0 40.0 19 37.93893363015343 40.0 38.0 40.0 32.0 40.0 20 37.93367380344399 40.0 38.0 40.0 32.0 40.0 21 37.9930159977302 40.0 38.0 40.0 32.0 40.0 22 37.984264169885854 40.0 38.0 40.0 32.0 40.0 23 37.95303258473559 40.0 38.0 40.0 32.0 40.0 24 37.92435452541522 40.0 38.0 40.0 32.0 40.0 25 37.95187585935965 40.0 38.0 40.0 32.0 40.0 26 37.88725201335691 40.0 38.0 40.0 32.0 40.0 27 37.82077304175124 40.0 38.0 40.0 32.0 40.0 28 37.83092167004954 40.0 38.0 40.0 32.0 40.0 29 37.783212204543965 40.0 38.0 40.0 32.0 40.0 30 37.783190379536876 40.0 38.0 40.0 32.0 40.0 31 37.7662759990397 40.0 38.0 40.0 32.0 40.0 32 37.74429821689692 40.0 38.0 40.0 32.0 40.0 33 37.70344180361859 40.0 38.0 40.0 32.0 40.0 34 37.70540605425697 40.0 38.0 40.0 32.0 40.0 35 37.70708657980314 40.0 38.0 40.0 32.0 40.0 36 37.66821624217028 40.0 38.0 40.0 32.0 40.0 37 37.677666470241604 40.0 38.0 40.0 32.0 40.0 38 37.65625613828325 40.0 38.0 40.0 32.0 40.0 39 37.59444771819551 40.0 38.0 40.0 32.0 40.0 40 37.580894388790675 40.0 38.0 40.0 32.0 40.0 41 37.60027499508937 40.0 38.0 40.0 32.0 40.0 42 37.578799188109734 40.0 38.0 40.0 32.0 40.0 43 37.56954538510225 40.0 38.0 40.0 32.0 40.0 44 37.48676313319802 40.0 38.0 40.0 32.0 40.0 45 37.42237936227329 40.0 38.0 40.0 32.0 40.0 46 37.3378729348087 40.0 38.0 40.0 32.0 40.0 47 37.30015932255178 40.0 38.0 40.0 32.0 40.0 48 37.26467186101836 40.0 38.0 40.0 32.0 40.0 49 37.1810166088304 40.0 38.0 40.0 32.0 40.0 50 37.22025797158384 40.0 38.0 40.0 32.0 40.0 51 36.76621052401842 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.2692988162033956 2201 2 -0.2113085629331053 2201 3 -0.5084888775906151 2201 4 0.6997070635752252 2201 5 0.6701238857540517 2201 6 0.464460086298935 2201 7 0.444779790603377 2201 8 0.26957396159917835 2201 9 0.7898336446800158 2201 10 0.3951831843462088 2201 11 0.09481820372200644 2201 12 0.46982285316148165 2201 13 0.04220235525873761 2201 14 0.8100657761977033 2201 15 0.30911716016469626 2201 16 0.30142514056141323 2201 17 0.866515283548658 2201 18 -0.011940856355892038 2201 19 0.47097726592564015 2201 20 0.8516934782820726 2201 21 0.1970305116479878 2201 22 -0.048021126123508395 2201 23 0.6648617556559344 2201 24 0.059822150657339535 2201 25 0.1898920250413667 2201 26 -0.15499214635709535 2201 27 -0.37622531355113153 2201 28 -1.5698210003958337 2201 29 -0.06605665390642912 2201 30 0.11043518778883055 2201 31 -0.7271099736057565 2201 32 -0.4158940106824147 2201 33 -0.68209793677471 2201 34 -0.06386890916929389 2201 35 -0.1614504045301146 2201 36 -0.00848601179007602 2201 37 0.28615498338005096 2201 38 0.47673123504132775 2201 39 0.9276419992130442 2201 40 0.8845955589633547 2201 41 -0.49073037579461243 2201 42 -0.8630304933247572 2201 43 -0.3373299912937 2201 44 -0.6760975919248082 2201 45 -0.6626337061181502 2201 46 -0.35190016696194704 2201 47 0.014968279798644346 2201 48 -0.009323155848967701 2201 49 -0.9819841479443738 2201 50 -0.5141583874973534 2201 51 -0.15097911546930476 1101 1 0.38579161302406106 1101 2 0.18958562484632324 1101 3 0.12489413284157536 1101 4 0.29856449030150145 1101 5 0.7255138509800787 1101 6 0.3285932209238709 1101 7 1.080895041472722 1101 8 0.4809500172375323 1101 9 0.748353267134064 1101 10 0.9947857675553493 1101 11 0.9358514873782369 1101 12 0.7442888243487644 1101 13 0.9664447795011597 1101 14 0.7574080514088308 1101 15 0.4285905829168044 1101 16 0.9770336850224197 1101 17 -0.07138834287955831 1101 18 0.856911602660503 1101 19 0.732775576904281 1101 20 0.289597104710289 1101 21 0.5611636462729237 1101 22 0.19167087586357923 1101 23 0.2125517705590667 1101 24 0.9209746593508257 1101 25 0.8051428943905918 1101 26 1.1530058665589493 1101 27 0.45288546637932114 1101 28 0.45874333144718094 1101 29 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1212 22 -1.0938851719875515 1212 23 -1.2601996694054876 1212 24 -1.3570492662140836 1212 25 -1.7134658783165406 1212 26 -0.9936489850139338 1212 27 -0.9859714232972436 1212 28 -0.8372247677996043 1212 29 -1.8166709045206844 1212 30 -1.3506622733086289 1212 31 -1.6071263536221352 1212 32 -0.26601686080526576 1212 33 -0.7578555125322879 1212 34 -0.9451137904141689 1212 35 -1.009116252195966 1212 36 0.04147303816897363 1212 37 -1.7490620518369866 1212 38 -0.7931295348194425 1212 39 -1.4605643889933475 1212 40 -1.9311292567614657 1212 41 -1.0153004003646373 1212 42 -1.1459952762895398 1212 43 0.2056675517038471 1212 44 -0.7510361668633863 1212 45 0.17929913581469492 1212 46 0.5772562621944815 1212 47 0.24224100707137097 1212 48 0.618440971915156 1212 49 0.14250397654375035 1212 50 -1.2750101483491179 1212 51 -1.4118308763210692 1213 1 -0.4352466383420648 1213 2 -0.3161065427310845 1213 3 -1.0690949381966774 1213 4 0.2603131241812804 1213 5 -1.1278559122257477 1213 6 -1.666853045014193 1213 7 -2.2963818255582424 1213 8 -1.1647694727442541 1213 9 -1.9374390825927108 1213 10 -1.481079441916421 1213 11 -0.646343412439613 1213 12 -1.6286619953233696 1213 13 -0.6863329982766189 1213 14 -1.5308433147113902 1213 15 -1.1845697085221758 1213 16 -1.5382213240850504 1213 17 -1.7938382518048783 1213 18 -1.3174964119114492 1213 19 -2.534073239124865 1213 20 -1.2657307641421696 1213 21 -1.1287270641095901 1213 22 -1.6111524392548162 1213 23 -1.8780675372733597 1213 24 -0.7545717887366052 1213 25 -2.1106130254636852 1213 26 -1.482012348377296 1213 27 -1.08582127314709 1213 28 -1.6266391822140207 1213 29 -1.8842384720882492 1213 30 -2.3074941051404636 1213 31 -1.9505948220906077 1213 32 -1.3211970409854459 1213 33 -1.9106332903100665 1213 34 -1.0903840606844426 1213 35 -2.0301372732169867 1213 36 -1.171364799668865 1213 37 -1.539602592377527 1213 38 -2.575036441726354 1213 39 -1.0243782028071635 1213 40 -2.2580812087134134 1213 41 -1.1346697697340034 1213 42 -0.758232513526778 1213 43 -1.3146027185664266 1213 44 -1.7442794101066283 1213 45 -1.7914215849060255 1213 46 -0.36831430837609247 1213 47 -2.132758992928629 1213 48 -2.5358383073641235 1213 49 -1.4554689964292251 1213 50 -1.4409260642650281 1213 51 -1.1471912366814294 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 1.0 17 0.0 18 1.0 19 4.0 20 4.0 21 4.0 22 24.0 23 38.0 24 88.0 25 139.0 26 223.0 27 303.0 28 450.0 29 540.0 30 692.0 31 881.0 32 1053.0 33 1248.0 34 1582.0 35 2011.0 36 2678.0 37 3964.0 38 7281.0 39 22609.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.7794271742927 19.84151239958086 13.364477820468041 29.0145826056584 2 14.441607193522337 21.656954538510227 40.40463563150658 23.496802636460856 3 19.10342870861433 24.02715030882385 30.190532311923 26.678888670638816 4 14.160064602020997 19.371876295859796 34.708308780200355 31.759750321918855 5 14.917392348152514 35.899954167485106 34.96366136318994 14.21899212117244 6 31.279600165870054 38.53641502433488 16.57609288723019 13.607891922564875 7 27.137213819594493 32.37303302123573 20.393286627818156 20.096466531351624 8 23.725965210938693 38.77649010235928 19.699251402256706 17.798293284445318 9 24.0358803116611 18.690936074554223 20.5897116916563 36.683471922128376 10 15.916977673017744 30.600842445273795 31.408367707719503 22.07381217398896 11 32.22096119429045 24.01894451962111 25.594744423588985 18.165349862499454 12 23.208712542831577 25.04201313865427 29.4703070778498 22.27896724066435 13 27.213601344420436 22.423012287478993 27.38383639974683 22.97954996835374 14 20.327811606538774 22.735109888910713 26.742181191208886 30.194897313341627 15 21.541282000916652 31.3385276850215 23.488072633623606 23.63211768043825 16 23.145420022261508 29.269517012593028 26.33841855998603 21.246644405159433 17 20.251424081712827 31.270870163032804 27.763591523167246 20.714114232087123 18 22.640068096994565 28.026714974791016 28.99578758975926 20.33742933845516 19 21.674414544184728 27.414391409677208 27.853074052249067 23.058119993888997 20 21.392871952683386 28.10406163382003 27.652283986992295 22.85078242650429 21 20.879984285994894 27.584626465003602 27.83343154586525 23.70195770313625 22 20.969466815076714 30.15779480128331 27.344551386979198 21.528186996660775 23 20.90835679521596 27.743949016783432 28.169536655099414 23.178157532901196 24 23.92457277548615 27.79851153451625 28.10406163382003 20.17285405617757 25 21.34267443636919 29.55105960409437 28.130251642331782 20.97601431720465 26 22.311704751304042 28.108426635238658 28.520919269298762 21.05894934415854 27 20.893079290250768 30.266919836748947 27.853074052249067 20.98692682075122 28 20.801414260459637 27.414391409677208 28.106244134529344 23.677950195333814 29 21.650407036382287 27.231061350094937 27.503873938759032 23.614657674763745 30 20.69710594089659 27.41498974202279 28.178881662228818 23.709022654851804 31 22.019249656256136 26.87749623518628 29.474672079268426 21.62858202928916 32 22.831139920120474 27.868351557214254 27.669743992666795 21.630764529998473 33 22.536502324363255 27.96001658700539 28.328859206879244 21.174621881752113 34 21.37322944629957 29.269517012593028 28.62349680263646 20.73375673847094 35 20.930181802309086 27.610816473515353 30.11850978851568 21.34049193565988 36 20.417294135620594 29.36991204522141 28.828651869311855 21.384141949846132 37 21.28592941792706 28.252471682053297 27.761409022457933 22.700189877561712 38 21.102599358344794 27.01717628058229 29.90898972042166 21.971234640651257 39 21.1898993867173 26.382068574172283 28.65623431327615 23.77179772583426 40 21.825007093127304 26.174731006787578 30.84310002400751 21.15716187607761 41 21.73115956262686 25.456688273423687 28.850476876404983 23.961675287544466 42 22.783124904515596 26.18564351033414 29.19094698705777 21.840284598092495 43 20.351819114341215 25.82334839258823 31.530587747441018 22.294244745629545 44 19.50500883912787 28.507824265042885 29.328444531744474 22.658722364084767 45 19.924048975315916 26.083065976996444 31.209760143172044 22.783124904515596 46 22.233134725768785 25.770968375564724 28.78936685654423 23.206530042122264 47 20.074641524258496 26.019773456426375 28.963966913289248 24.941618106025885 48 19.714528907221897 26.194373513171392 28.859206879242237 25.231890700364477 49 21.873022108732183 25.82334839258823 28.86357188066086 23.440057618018724 50 19.254021257556907 26.377703572753664 30.62921495449486 23.73906021519457 51 18.666928566751785 26.82511621816277 28.560204282066394 25.947750933019055 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 134.0 1 77.5 2 21.0 3 20.0 4 19.0 5 24.0 6 29.0 7 30.5 8 32.0 9 45.5 10 59.0 11 77.0 12 95.0 13 157.0 14 219.0 15 247.5 16 276.0 17 258.0 18 240.0 19 215.0 20 190.0 21 177.0 22 164.0 23 155.5 24 147.0 25 169.5 26 226.5 27 261.0 28 439.5 29 618.0 30 996.5 31 1375.0 32 1989.0 33 2603.0 34 2986.0 35 3369.0 36 3482.5 37 3596.0 38 3381.5 39 3167.0 40 3069.0 41 2971.0 42 2891.5 43 2812.0 44 2818.5 45 2825.0 46 2717.0 47 2609.0 48 2678.5 49 2748.0 50 2756.5 51 2765.0 52 3047.5 53 3330.0 54 2756.0 55 2182.0 56 1976.0 57 1770.0 58 1576.5 59 1383.0 60 1260.5 61 1138.0 62 994.5 63 851.0 64 713.5 65 576.0 66 514.0 67 452.0 68 338.5 69 225.0 70 194.5 71 164.0 72 126.5 73 89.0 74 69.0 75 46.5 76 44.0 77 28.0 78 12.0 79 9.0 80 6.0 81 6.0 82 6.0 83 5.0 84 4.0 85 2.5 86 1.0 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024007507802440035 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0021825007093127305 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.004365001418625461 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0021825007093127305 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 45819.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.25232337557313 #Duplication Level Percentage of deduplicated Percentage of total 1 95.60608698827086 87.24277566526868 2 3.7014256886281944 6.75527387778707 3 0.3521733646541516 0.9640991326705283 4 0.1422385198693065 0.5191838164630735 5 0.07139643641492266 0.3257545351799034 6 0.03401465175982206 0.1862349601136874 7 0.018941725195518327 0.12099335029828756 8 0.002981839592363567 0.02176798325891579 9 0.0057051413299432 0.04685466613889919 >10 0.050694757084687854 0.8529959313449289 >50 0.007170449893233402 0.4492998126464062 >100 0.004780291538001603 0.5409037594764498 >500 0.002390145768968376 1.9738625093531743 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC 905 1.975163141928021 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.27499508937340406 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 122 0.2662650865361531 No Hit TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT 74 0.16150505248914204 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71 0.15495755036120387 No Hit CAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAA 61 0.13313254326807655 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47 0.10257753333769834 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0021825007093127305 13 0.0 0.0 0.0021825007093127305 14 0.0 0.0 0.0021825007093127305 15 0.0 0.0 0.0021825007093127305 16 0.0 0.0 0.0021825007093127305 17 0.0 0.0 0.0021825007093127305 18 0.0 0.0 0.0021825007093127305 19 0.0 0.0 0.0065475021279381915 20 0.0 0.0 0.008730002837250922 21 0.0 0.0 0.008730002837250922 22 0.0 0.0 0.008730002837250922 23 0.0 0.0 0.013095004255876383 24 0.0 0.0 0.024007507802440035 25 0.0 0.0 0.026190008511752766 26 0.0 0.0 0.04146751347694188 27 0.0 0.0 0.05674501844213099 28 0.0 0.0 0.06984002269800738 29 0.0 0.0 0.10476003404701106 30 0.0 0.0 0.18114755887295664 31 0.0 0.0 0.4365001418625461 32 0.0 0.0 0.7464152425849538 33 0.0 0.0 1.1283528667146816 34 0.0 0.0 1.5168379929723477 35 0.0 0.0 1.887863113555512 36 0.0 0.0 2.4160282852091925 37 0.0 0.0 3.1166110128985793 38 0.0 0.0 3.9808812937864206 39 0.0 0.0 4.897531591697767 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 115 0.0 41.08696 45 ATCTCCG 120 0.0 39.375004 10 CGTATGC 120 0.0 39.375004 41 CTCCGAG 120 0.0 39.375004 12 TATGCCG 120 0.0 39.375004 43 CCGAGCC 120 0.0 39.375004 14 CGAGCCC 120 0.0 39.375004 15 TCTCCGA 120 0.0 39.375004 11 GGATAAT 105 0.0 38.571426 32 ATCTCGT 125 0.0 37.8 37 CTCGTAT 125 0.0 37.8 39 TCCGAGC 125 0.0 37.8 13 CACGAGA 125 0.0 37.8 21 CCCACGA 125 0.0 37.8 19 GTATGCC 125 0.0 37.8 42 TCTCGTA 125 0.0 37.8 38 TCGTATG 125 0.0 37.8 40 ATGCCGT 125 0.0 37.8 44 ACGAGAC 125 0.0 37.8 22 CCACGAG 125 0.0 37.8 20 >>END_MODULE