FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_94.34100000006df1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_94.34100000006df1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45819
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC9051.975163141928021No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.27499508937340406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.2662650865361531No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT740.16150505248914204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.15495755036120387No Hit
CAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAA610.13313254326807655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.10257753333769834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1150.041.0869645
ATCTCCG1200.039.37500410
CGTATGC1200.039.37500441
CTCCGAG1200.039.37500412
TATGCCG1200.039.37500443
CCGAGCC1200.039.37500414
CGAGCCC1200.039.37500415
TCTCCGA1200.039.37500411
GGATAAT1050.038.57142632
ATCTCGT1250.037.837
CTCGTAT1250.037.839
TCCGAGC1250.037.813
CACGAGA1250.037.821
CCCACGA1250.037.819
GTATGCC1250.037.842
TCTCGTA1250.037.838
TCGTATG1250.037.840
ATGCCGT1250.037.844
ACGAGAC1250.037.822
CCACGAG1250.037.820