##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_91.34100000006dca.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38863 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.274091037747986 38.0 38.0 38.0 32.0 38.0 2 36.2681728121864 38.0 38.0 38.0 32.0 38.0 3 36.28258240485809 38.0 38.0 38.0 32.0 38.0 4 36.37279674754908 38.0 38.0 38.0 32.0 38.0 5 36.40876926639734 38.0 38.0 38.0 32.0 38.0 6 38.299076242184086 40.0 38.0 40.0 38.0 40.0 7 38.221084321848544 40.0 38.0 40.0 38.0 40.0 8 38.15299899647479 40.0 38.0 40.0 38.0 40.0 9 38.20212026863598 40.0 38.0 40.0 38.0 40.0 10 38.1333916578751 40.0 38.0 40.0 38.0 40.0 11 38.162905591436584 40.0 38.0 40.0 38.0 40.0 12 38.04876103234439 40.0 38.0 40.0 38.0 40.0 13 38.09857705272367 40.0 38.0 40.0 38.0 40.0 14 38.07166199212619 40.0 38.0 40.0 38.0 40.0 15 38.04670251910557 40.0 38.0 40.0 32.0 40.0 16 38.03646141574248 40.0 38.0 40.0 32.0 40.0 17 38.01410081568587 40.0 38.0 40.0 32.0 40.0 18 38.04266268687441 40.0 38.0 40.0 32.0 40.0 19 38.05413889818079 40.0 38.0 40.0 32.0 40.0 20 38.077425829194866 40.0 38.0 40.0 32.0 40.0 21 38.095669402773844 40.0 38.0 40.0 38.0 40.0 22 38.044798394359674 40.0 38.0 40.0 32.0 40.0 23 38.07948434243368 40.0 38.0 40.0 38.0 40.0 24 38.024187530556055 40.0 38.0 40.0 32.0 40.0 25 38.04250829838149 40.0 38.0 40.0 32.0 40.0 26 37.97686745747884 40.0 38.0 40.0 32.0 40.0 27 37.98983609088336 40.0 38.0 40.0 32.0 40.0 28 37.94004580191957 40.0 38.0 40.0 32.0 40.0 29 37.91045467411162 40.0 38.0 40.0 32.0 40.0 30 37.89581349870056 40.0 38.0 40.0 32.0 40.0 31 37.89671409824255 40.0 38.0 40.0 32.0 40.0 32 37.89509301906698 40.0 38.0 40.0 32.0 40.0 33 37.8366055116692 40.0 38.0 40.0 32.0 40.0 34 37.88572678383038 40.0 38.0 40.0 32.0 40.0 35 37.86092169930268 40.0 38.0 40.0 32.0 40.0 36 37.80783778915678 40.0 38.0 40.0 32.0 40.0 37 37.847386974757484 40.0 38.0 40.0 32.0 40.0 38 37.824254432236316 40.0 38.0 40.0 32.0 40.0 39 37.7772688675604 40.0 38.0 40.0 32.0 40.0 40 37.75390474229987 40.0 38.0 40.0 32.0 40.0 41 37.748552607878956 40.0 38.0 40.0 32.0 40.0 42 37.67709646707665 40.0 38.0 40.0 32.0 40.0 43 37.62807812057741 40.0 38.0 40.0 32.0 40.0 44 37.578030517458764 40.0 38.0 40.0 32.0 40.0 45 37.54805341841855 40.0 38.0 40.0 32.0 40.0 46 37.46699945964028 40.0 38.0 40.0 32.0 40.0 47 37.42469701258266 40.0 38.0 40.0 32.0 40.0 48 37.34045235828423 40.0 38.0 40.0 32.0 40.0 49 37.31415485165839 40.0 38.0 40.0 32.0 40.0 50 37.31011501942722 40.0 38.0 40.0 32.0 40.0 51 36.853511051642954 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.22132497220875536 2201 2 -0.7599715240889751 2201 3 -0.2317867169492942 2201 4 -0.4429736093836638 2201 5 -0.3981204044981723 2201 6 -1.28450706120406 2201 7 -1.0654948306675749 2201 8 -0.7138576375201424 2201 9 -0.6455365495680496 2201 10 -0.997507045596457 2201 11 0.03485960224359275 2201 12 -0.5729751796081359 2201 13 -1.0911915571556534 2201 14 -0.7520182749546507 2201 15 -1.2588177156908955 2201 16 -1.2573480071654544 2201 17 -0.6370295178033842 2201 18 -0.1980467893009461 2201 19 -0.9590357614705383 2201 20 -0.5175199138313786 2201 21 -0.25609145926573973 2201 22 -0.6116978649989804 2201 23 -1.092268745751845 2201 24 -2.264133809385683 2201 25 -0.38909501453476025 2201 26 -0.6592213044091366 2201 27 -0.49923886569361997 2201 28 -1.8073795411614597 2201 29 -0.9644594411644647 2201 30 -0.9255377276265406 2201 31 -0.7051489352890883 2201 32 -1.0254426707206292 2201 33 -1.224318161648199 2201 34 -1.3606300458198959 2201 35 -0.23664311425088158 2201 36 -1.084646963883074 2201 37 -1.6583757074846304 2201 38 -1.9452489650452662 2201 39 -1.9733351710431748 2201 40 -1.8072227690140394 2201 41 -0.7923809997022744 2201 42 -1.0201082335808778 2201 43 -0.024420828802853123 2201 44 -1.4308301098464682 2201 45 -2.513870921011069 2201 46 -1.885482878285707 2201 47 -1.2326729123841247 2201 48 -3.204862194081926 2201 49 -1.273971083078365 2201 50 -0.7341758899970543 2201 51 -1.3051078056047984 1101 1 0.4346204468945629 1101 2 0.7897360782502076 1101 3 0.405640183635505 1101 4 0.6262271703434337 1101 5 0.7373571783478283 1101 6 0.4767015157939909 1101 7 0.26393986718817786 1101 8 0.8533938244681636 1101 9 0.21996052645533837 1101 10 0.9917717068401899 1101 11 0.22881671725334485 1101 12 -0.006698376489225666 1101 13 0.7440910939164738 1101 14 0.8805353312831699 1101 15 0.5832875474669947 1101 16 1.025303064959303 1101 17 0.7898710669919353 1101 18 0.8458128598218622 1101 19 0.5039272014924236 1101 20 0.972733497474664 1101 21 0.25073115282003045 1101 22 0.41364326560530884 1101 23 0.40090864216239197 1101 24 0.33138275981509935 1101 25 0.4461876365373669 1101 26 0.2842484811659105 1101 27 0.9754200037020908 1101 28 0.5337510631270419 1101 29 0.5881721377829052 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41 -0.49032150313476563 1213 42 -0.3938687217578405 1213 43 -1.2875787235396956 1213 44 -0.9449414752164174 1213 45 -0.3399578775328109 1213 46 -0.1883051513596925 1213 47 -0.9435806164268428 1213 48 -0.3505525068202928 1213 49 -0.4944134934521287 1213 50 -1.0019104437727933 1213 51 -0.6773427407687933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 4.0 16 1.0 17 0.0 18 0.0 19 1.0 20 3.0 21 10.0 22 16.0 23 21.0 24 50.0 25 105.0 26 169.0 27 233.0 28 311.0 29 421.0 30 548.0 31 656.0 32 887.0 33 1062.0 34 1340.0 35 1634.0 36 2179.0 37 3293.0 38 6105.0 39 19813.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.211799835255356 19.15671334431631 13.179571663920923 28.45191515650741 2 14.919458597087129 21.478565179352582 39.72003499562555 23.881941227934746 3 19.10814913928415 23.46190463937421 29.706919177623963 27.723027043717675 4 13.478115431129867 18.98978462805239 35.14911355273654 32.38298638808121 5 14.226899621748194 36.340478089699715 34.83004400072048 14.602578287831614 6 31.95841803257597 38.00272753004143 16.293132285207008 13.74572215217559 7 27.30617811285799 31.950698607930423 20.168283457272985 20.574839821938603 8 24.071739186372643 37.665645987185755 19.602192316599336 18.660422509842267 9 24.16179914057072 18.194683889560764 20.35354964876618 37.28996732110234 10 15.57007951007385 30.813370043486092 31.299693796155726 22.316856650284333 11 32.74579934642205 24.717597715050303 24.591513779172992 17.94508915935466 12 22.466098860098292 25.767439466845072 29.120242904562183 22.646218768494457 13 26.91248745593495 22.625633636106322 27.4013843501531 23.060494557805626 14 20.582559246584154 22.692535316367753 26.997401127035996 29.727504310012094 15 21.88971515323058 30.491727349921522 23.55196459357229 24.06659290327561 16 23.542277803509855 29.607328495702745 25.56996551901601 21.280428181771395 17 20.783264287368446 30.159792090162878 27.62782080642256 21.42912281604611 18 22.494403417131974 27.92115894295345 28.906672156035302 20.677765483879266 19 21.99521395671976 27.445127756477884 28.08069371896148 22.478964567840876 20 21.665851838509635 27.56091912616113 28.512981499112268 22.26024753621697 21 20.99940817744384 27.457993464220465 28.574736896276665 22.967861462059027 22 21.133211537966705 29.640017497362532 28.173326814708076 21.05344414996269 23 21.26958804003808 27.658698505004757 27.635540231068113 23.436173223889046 24 22.931837480379798 27.4013843501531 28.69052826595991 20.976249903507192 25 21.318477729459897 29.295216529861307 28.338007873813137 21.048297866865656 26 22.548439389650824 27.735892751460256 28.46409180969045 21.251576049198466 27 21.441988523788694 29.31580166224944 28.165607390062526 21.076602423899338 28 20.94794534647351 27.609808815582948 28.02665774644263 23.415588091500915 29 20.878470524663562 27.823379564109825 27.957182924632683 23.340966986593934 30 21.58573303481819 27.52000823490054 28.165933245837515 22.728325484443758 31 21.351928569590616 27.146643336849962 29.95394076628155 21.547487327277874 32 23.304943004914698 27.23670329104804 28.27625247664874 21.182101227388518 33 22.86236265856985 27.885134961274222 28.394616987880504 20.85788539227543 34 21.421403391400563 28.983866402490797 28.394616987880504 21.200113218228132 35 21.305612021717316 27.290739263566888 30.30903429997684 21.094614414738956 36 20.829580835241746 29.503640995291153 28.36116614774979 21.305612021717316 37 20.937652780279443 28.695674549056942 28.296837609036874 22.06983506162674 38 20.657180351491135 26.956230862259734 30.638396418186964 21.748192368062167 39 21.207832642873683 26.884182898901273 28.46666495123897 23.44131950698608 40 21.681290687800736 26.47505339268713 30.574067879474047 21.26958804003808 41 21.60152329979672 26.616576177855546 28.47953065898155 23.302369863366184 42 22.780022129017315 26.235751228675092 28.9529887039086 22.03123793839899 43 20.96081105421609 26.747806396829887 30.520031906955204 21.771350641998815 44 20.731801456398117 28.38947070478347 28.615907161052927 22.262820677765486 45 20.664899776136686 26.570259629982246 30.49430049147003 22.270540102411037 46 22.756863855080667 26.531662506754493 28.222216504129893 22.489257134034943 47 20.976789665997632 26.54006484483557 28.176624980700943 24.306520508465855 48 20.340683941023595 26.63716131024368 28.723979106090624 24.298175642642104 49 22.494403417131974 26.601137328564445 28.73684481383321 22.16761444047037 50 20.39729305509096 26.83014692638242 30.30903429997684 22.463525718549775 51 20.469867737121096 26.841130152848542 27.82924193299367 24.859760177036694 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 214.0 1 122.0 2 30.0 3 29.5 4 29.0 5 26.5 6 24.0 7 25.5 8 27.0 9 27.0 10 27.0 11 61.5 12 96.0 13 162.0 14 228.0 15 260.0 16 292.0 17 270.0 18 248.0 19 217.5 20 187.0 21 199.5 22 212.0 23 226.0 24 240.0 25 280.5 26 392.5 27 464.0 28 564.0 29 664.0 30 839.0 31 1014.0 32 1211.0 33 1408.0 34 1627.5 35 1847.0 36 2053.5 37 2260.0 38 2336.0 39 2412.0 40 2498.0 41 2584.0 42 2631.5 43 2679.0 44 2687.5 45 2696.0 46 2762.5 47 2829.0 48 2781.0 49 2733.0 50 2711.5 51 2690.0 52 2862.0 53 3034.0 54 2485.5 55 1937.0 56 1753.0 57 1569.0 58 1402.5 59 1236.0 60 1078.0 61 920.0 62 778.0 63 636.0 64 540.0 65 444.0 66 354.0 67 264.0 68 210.5 69 157.0 70 120.5 71 84.0 72 69.5 73 55.0 74 44.5 75 26.0 76 18.0 77 14.0 78 10.0 79 7.5 80 5.0 81 5.0 82 5.0 83 2.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03859712322774876 2 0.002573141548516584 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.002573141548516584 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.010292566194066335 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.002573141548516584 48 0.0 49 0.0 50 0.0 51 0.002573141548516584 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 38863.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.04026589683261 #Duplication Level Percentage of deduplicated Percentage of total 1 96.80612462621225 90.06867575664704 2 2.823865210064584 5.254663400024479 3 0.2623828492099532 0.7323651017178775 4 0.026740068331655542 0.09951612270706818 5 0.019690671624525997 0.09160126618165577 6 0.010825526154279296 0.06043258991203569 7 0.010158019704376521 0.06615733979963197 8 0.003229664174141063 0.0240391050815647 9 0.0031166866680321847 0.0260979620679745 >10 0.0172849884419635 0.3032194858942511 >50 0.008290847352695935 0.5373965116137646 >100 0.0055272280410061546 0.9256585798937554 >500 0.0027636140205030773 1.8101767784588991 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC 704 1.8114916501556748 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 208 0.5352134420914495 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 152 0.3911175153745208 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 94 0.24187530556055886 No Hit TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT 59 0.15181535136247845 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56 0.1440959267169287 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 0.10549880348917995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.002573141548516584 3 0.0 0.0 0.002573141548516584 4 0.0 0.0 0.002573141548516584 5 0.0 0.0 0.002573141548516584 6 0.0 0.0 0.002573141548516584 7 0.0 0.0 0.002573141548516584 8 0.0 0.0 0.002573141548516584 9 0.0 0.0 0.002573141548516584 10 0.0 0.0 0.002573141548516584 11 0.0 0.0 0.002573141548516584 12 0.0 0.0 0.002573141548516584 13 0.0 0.0 0.002573141548516584 14 0.0 0.0 0.002573141548516584 15 0.0 0.0 0.002573141548516584 16 0.0 0.0 0.002573141548516584 17 0.0 0.0 0.002573141548516584 18 0.0 0.0 0.005146283097033168 19 0.0 0.0 0.005146283097033168 20 0.0 0.0 0.005146283097033168 21 0.0 0.0 0.005146283097033168 22 0.0 0.0 0.005146283097033168 23 0.0 0.0 0.005146283097033168 24 0.0 0.0 0.005146283097033168 25 0.0 0.0 0.005146283097033168 26 0.0 0.0 0.005146283097033168 27 0.0 0.0 0.005146283097033168 28 0.0 0.0 0.018011990839616087 29 0.0 0.0 0.033450840130715594 30 0.0 0.0 0.08234052955253068 31 0.0 0.0 0.21871703162390962 32 0.0 0.0 0.40912950621413685 33 0.0 0.0 0.5712374237706817 34 0.0 0.0 0.7796618892005249 35 0.0 0.0 1.0266834778581169 36 0.0 0.0 1.304582765097908 37 0.0 0.0 1.687980855826879 38 0.0 0.0 2.1665851838509638 39 0.0 0.0 2.6554820780691144 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGCC 135 0.0 40.000004 42 TATGCCG 135 0.0 40.000004 43 GATAATC 135 0.0 40.000004 33 ATAATCT 135 0.0 40.000004 34 TCTCGTA 135 0.0 40.000004 38 ACGAGAC 135 0.0 40.000004 22 TCGTATG 135 0.0 40.000004 40 CGAGACC 135 0.0 40.000004 23 CTCGTAT 135 0.0 40.000004 39 CTCCGAG 135 0.0 40.000004 12 GCCCACG 145 0.0 38.793102 18 AATCTCG 140 0.0 38.571426 36 ATCTCGT 140 0.0 38.571426 37 TGCCGTC 140 0.0 38.571426 45 GGATAAT 140 0.0 38.571426 32 CTGGATA 140 0.0 38.571426 30 ATGCCGT 140 0.0 38.571426 44 CCCTGGA 140 0.0 38.571426 28 CGTATGC 140 0.0 38.571426 41 TCTCCGA 140 0.0 38.571426 11 >>END_MODULE