FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_91.34100000006dca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_91.34100000006dca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38863
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC7041.8114916501556748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.5352134420914495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.3911175153745208No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.24187530556055886No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT590.15181535136247845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.1440959267169287No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.10549880348917995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1350.040.00000442
TATGCCG1350.040.00000443
GATAATC1350.040.00000433
ATAATCT1350.040.00000434
TCTCGTA1350.040.00000438
ACGAGAC1350.040.00000422
TCGTATG1350.040.00000440
CGAGACC1350.040.00000423
CTCGTAT1350.040.00000439
CTCCGAG1350.040.00000412
GCCCACG1450.038.79310218
AATCTCG1400.038.57142636
ATCTCGT1400.038.57142637
TGCCGTC1400.038.57142645
GGATAAT1400.038.57142632
CTGGATA1400.038.57142630
ATGCCGT1400.038.57142644
CCCTGGA1400.038.57142628
CGTATGC1400.038.57142641
TCTCCGA1400.038.57142611