FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_90.34100000006dbd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_90.34100000006dbd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24495
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9313.8007756685037766No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC6722.743417023882425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3311.3512961828944683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.7307613798734436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.48581343131251276No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.38375178607879157No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT810.3306797305572566No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.2286180853235354No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.14696876913655849No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.13063890589916308No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.12655644008981423No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.10614411104307002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT302.0838943E-644.98568345
GTATCAA1150.033.2502861
CAACGCA1250.030.652955
TATCAAC1250.030.5902632
TCAACGC1300.029.4137174
AACGCAG1350.028.382366
ATCAACG1400.027.3127383
TGCCGTC708.723928E-725.70610645
ACGCAGA1500.025.5441257
CGCAGAG1500.025.5441258
AGAGTAC1550.024.72012311
AATCTCG751.5917412E-623.99236536
ATCTCGT751.5917412E-623.99236537
CAGAGTA1600.023.94761810
GCAGAGT1650.023.2219339
ACATCTC802.7405604E-622.5389378
TCTCCGA802.7405604E-622.53893711
GTATGCC702.5723475E-522.49284442
GCCCACG802.7901242E-622.49284418
TATGCCG702.5723475E-522.49284443
Produced by FastQC (version 0.11.2)