##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_90.34100000006dbd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24495 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.26356399265156 38.0 38.0 38.0 32.0 38.0 2 36.32525005103082 38.0 38.0 38.0 32.0 38.0 3 36.3109614206981 38.0 38.0 38.0 32.0 38.0 4 36.34570320473566 38.0 38.0 38.0 32.0 38.0 5 36.423270055113285 38.0 38.0 38.0 32.0 38.0 6 38.32818942641355 40.0 38.0 40.0 38.0 40.0 7 38.22118799755052 40.0 38.0 40.0 38.0 40.0 8 38.185343947744435 40.0 38.0 40.0 38.0 40.0 9 38.191753419065115 40.0 38.0 40.0 38.0 40.0 10 38.143621147172894 40.0 38.0 40.0 38.0 40.0 11 38.138844662175956 40.0 38.0 40.0 38.0 40.0 12 38.0106552357624 40.0 38.0 40.0 32.0 40.0 13 37.974607062665854 40.0 38.0 40.0 32.0 40.0 14 37.985139824453974 40.0 38.0 40.0 32.0 40.0 15 37.95684833639518 40.0 38.0 40.0 32.0 40.0 16 37.99330475607267 40.0 38.0 40.0 32.0 40.0 17 38.01698305776689 40.0 38.0 40.0 32.0 40.0 18 37.980322514798935 40.0 38.0 40.0 32.0 40.0 19 38.06356399265156 40.0 38.0 40.0 32.0 40.0 20 38.01322718922229 40.0 38.0 40.0 32.0 40.0 21 38.03874260053072 40.0 38.0 40.0 38.0 40.0 22 38.02306593182282 40.0 38.0 40.0 32.0 40.0 23 38.00759338640539 40.0 38.0 40.0 32.0 40.0 24 37.94709124311084 40.0 38.0 40.0 32.0 40.0 25 37.96729944886712 40.0 38.0 40.0 32.0 40.0 26 37.91839150847112 40.0 38.0 40.0 32.0 40.0 27 37.91014492753623 40.0 38.0 40.0 32.0 40.0 28 37.858093488467034 40.0 38.0 40.0 32.0 40.0 29 37.85041845274546 40.0 38.0 40.0 32.0 40.0 30 37.850377628087365 40.0 38.0 40.0 32.0 40.0 31 37.852908756889164 40.0 38.0 40.0 32.0 40.0 32 37.82988364972444 40.0 38.0 40.0 32.0 40.0 33 37.810042865891 40.0 38.0 40.0 32.0 40.0 34 37.80885895080629 40.0 38.0 40.0 32.0 40.0 35 37.771055317411715 40.0 38.0 40.0 32.0 40.0 36 37.7379465196979 40.0 38.0 40.0 32.0 40.0 37 37.6927944478465 40.0 38.0 40.0 32.0 40.0 38 37.66691161461523 40.0 38.0 40.0 32.0 40.0 39 37.5786895284752 40.0 38.0 40.0 32.0 40.0 40 37.556603388446625 40.0 38.0 40.0 32.0 40.0 41 37.5742804654011 40.0 38.0 40.0 32.0 40.0 42 37.551500306184934 40.0 38.0 40.0 32.0 40.0 43 37.48315982853644 40.0 38.0 40.0 32.0 40.0 44 37.41110430700143 40.0 38.0 40.0 32.0 40.0 45 37.31312512757706 40.0 38.0 40.0 32.0 40.0 46 37.143906919779546 40.0 38.0 40.0 32.0 40.0 47 37.027801592161666 40.0 38.0 40.0 32.0 40.0 48 36.99016125739947 40.0 38.0 40.0 32.0 40.0 49 36.90124515207185 40.0 38.0 40.0 32.0 40.0 50 36.91185956317616 40.0 38.0 40.0 32.0 40.0 51 36.501653398652785 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.2637573037152876 2201 2 0.23067870621046893 2201 3 -0.4293007059618077 2201 4 -0.1290790229674954 2201 5 0.18292921048080757 2201 6 -0.21199859503635565 2201 7 0.17530659089009504 2201 8 -0.7919203139614268 2201 9 -0.4190588576103025 2201 10 -0.8903998264114747 2201 11 -0.5401277345651394 2201 12 -0.19568635528896294 2201 13 -0.19271742048039897 2201 14 -0.45032823754967666 2201 15 -0.20933816189076282 2201 16 0.5223396869645711 2201 17 -0.3093005166705325 2201 18 0.37885676978648775 2201 19 -0.5883842690617342 2201 20 0.7903982039316872 2201 21 0.30470345495195517 2201 22 0.29070056655339727 2201 23 0.4856253266528654 2201 24 1.0480875609056568 2201 25 0.4950337373198437 2201 26 0.8362755188075823 2201 27 0.38942942146071147 2201 28 0.21594962427390385 2201 29 -1.1112695771698213 2201 30 -0.5646324872423776 2201 31 0.40718608194845274 2201 32 0.40995842686348993 2201 33 0.45301860655561654 2201 34 0.5164547444994199 2201 35 0.24197703282037963 2201 36 -0.4205479190882073 2201 37 -1.4299718964460908 2201 38 -0.5758311931160094 2201 39 -0.2693907409669123 2201 40 0.6248564160751755 2201 41 0.0799372732446173 2201 42 0.514333550276568 2201 43 -0.14211632607754865 2201 44 0.4481715701971609 2201 45 0.18218242222582148 2201 46 -0.9039125848509642 2201 47 -0.5207151578496649 2201 48 -1.7680905291597924 2201 49 0.1267776824685498 2201 50 0.4296669861444258 2201 51 0.7425158204650586 1101 1 -0.03456202401579844 1101 2 -0.5819263358063367 1101 3 -1.2545107899954218 1101 4 -0.6794991910347221 1101 5 0.4526771096404687 1101 6 -0.87166246058257 1101 7 -0.2625085351603218 1101 8 0.7988359885595813 1101 9 0.5019495457510388 1101 10 -0.013088902041729966 1101 11 0.09936806375419138 1101 12 -0.4746779519276174 1101 13 0.5568624114523715 1101 14 0.6765625187528386 1101 15 0.6259559557562966 1101 16 0.22065901469566285 1101 17 0.12683393711098034 1101 18 -1.142991969709314 1101 19 -0.34972880687686114 1101 20 -0.9751480145557068 1101 21 -1.4171452845438424 1101 22 0.5974232556290247 1101 23 -2.441265429649654 1101 24 0.5060707541829714 1101 25 0.7362102079080728 1101 26 0.0438385440176674 1101 27 0.09615211053633743 1101 28 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1.336694344225485 2202 14 0.6673188212738523 2202 15 -0.4446322795378208 2202 16 0.22822203990574508 2202 17 0.7201112480353515 2202 18 -0.0034961713899832603 2202 19 0.4704392603500267 2202 20 -0.4154841490094938 2202 21 1.1576446314225421 2202 22 0.9083476253769263 2202 23 0.3385665031234595 2202 24 0.8716169726703669 2202 25 1.1126807961433727 2202 26 0.7774519893958214 2202 27 1.3011941273430665 2202 28 1.392420212509201 2202 29 -0.2877401654051184 2202 30 0.20007339511056443 2202 31 0.14248019959551073 2202 32 0.29231136803996094 2202 33 0.5118421359673775 2202 34 -0.012957020206464165 2202 35 0.6537417387027347 2202 36 0.16768737502943765 2202 37 0.36414575061272814 2202 38 1.4535805715898746 2202 39 -0.09292015273162235 2202 40 1.2719152396045885 2202 41 0.37405492030343623 2202 42 -0.6621370379587219 2202 43 0.06376602686362531 2202 44 -0.757710782744013 2202 45 0.6527706575199375 2202 46 0.6549109445607968 2202 47 -0.5207151578496649 2202 48 0.2024977061343236 2202 49 0.3620718001156078 2202 50 -0.45268595503204523 2202 51 0.6248687616415296 1102 1 0.32258083312705566 1102 2 -1.0716088754888773 1102 3 0.3550130195283856 1102 4 -0.3937849053204374 1102 5 0.42966123662459665 1102 6 0.14421055529044224 1102 7 0.37955495690317065 1102 8 0.9123280520516488 1102 9 -0.23932029551880163 1102 10 -0.6567396956925222 1102 11 -1.1299970156108898 1102 12 -0.5159477931974621 1102 13 -0.20742330283334098 1102 14 0.1575148997052196 1102 15 0.3315115113118523 1102 16 0.5468494908861388 1102 17 0.8998498101268524 1102 18 -0.8580713347886757 1102 19 0.042334685186631305 1102 20 0.3296138902061969 1102 21 0.6511086837101274 1102 22 -0.6962275380217662 1102 23 -0.6499955883798094 1102 24 0.6886104367226551 1102 25 -0.17490090320303864 1102 26 0.68431473449386 1102 27 -0.041943127558894844 1102 28 -0.7252268463143281 1102 29 -0.3710734987384541 1102 30 7.269898818051956E-4 1102 31 0.8107808531902805 1102 32 -0.49200235745023235 1102 33 0.6392931163595392 1102 34 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0.32659363764081206 1215 11 0.33111409550021875 1215 12 0.06182998458032074 1215 13 0.109243363833329 1215 14 0.257514899705221 1215 15 0.15928928908962803 1215 16 -0.5475949535583098 1215 17 0.3554053656824081 1215 18 -0.21918244589978997 1215 19 -0.03544309259114442 1215 20 -0.9148305542382502 1215 21 -0.4044468718454297 1215 22 0.2482169064226767 1215 23 -0.4222178106020351 1215 24 -0.8113895632773449 1215 25 0.3139879856858556 1215 26 0.38987029004941576 1215 27 0.5691679835522123 1215 28 0.8081064870190033 1215 29 -0.25440683207178694 1215 30 0.8007269898818095 1215 31 0.46078085319028617 1215 32 1.107997642549762 1215 33 0.7504042274706464 1215 34 -0.9260289156312993 1215 35 0.4491665753040408 1215 36 0.33696842078107636 1215 37 0.36479934538397174 1215 38 0.646391029106212 1215 39 0.7096942263533492 1215 40 -0.14442462967645753 1215 41 0.6034666850093231 1215 42 1.2705427006033645 1215 43 -0.12512286202526468 1215 44 0.2991519623540242 1215 45 0.8292412457552345 1215 46 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 2.0 19 2.0 20 2.0 21 7.0 22 8.0 23 19.0 24 53.0 25 65.0 26 109.0 27 162.0 28 222.0 29 312.0 30 394.0 31 436.0 32 550.0 33 721.0 34 835.0 35 1081.0 36 1426.0 37 2115.0 38 3709.0 39 12264.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.33050155203398 21.724391439307304 13.241300441104395 27.703806567554324 2 13.766074709124313 24.090630740967544 39.41212492345377 22.731169626454378 3 18.411920800163298 26.878954888752805 29.744845886915698 24.9642784241682 4 12.010614411104308 23.061849357011635 34.100836905490915 30.826699326393143 5 13.186364564196776 38.497652582159624 33.6476832006532 14.668299652990408 6 29.389671361502344 41.57583180240865 15.492957746478872 13.541539089610124 7 24.4294754031435 35.750153092467855 19.518269034496836 20.302102469891814 8 22.923045519493773 39.54276382935293 18.946723821187998 18.587466829965297 9 23.270055113288425 20.702184119208 19.68973259848949 36.33802816901409 10 14.962237191263522 33.79056950398041 29.60195958358849 21.645233721167585 11 30.543420569142203 27.138366063773322 23.99461070509942 18.323602661985056 12 22.51479893855889 28.20167381098183 27.670953255766484 21.612573994692795 13 25.87058583384364 25.56031843233313 27.50765462339253 21.0614411104307 14 19.804041641151255 24.845886915697083 27.107572974076344 28.242498469075322 15 21.122678097570933 31.84731577873035 24.409063074096753 22.620943049601962 16 21.70647070830782 32.81486017554603 25.397019799959175 20.08164931618698 17 18.82833231271688 33.32925086752398 27.246376811594203 20.59604000816493 18 21.265564400898143 29.14064094713207 30.181669728516024 19.412124923453767 19 19.18758930393958 29.357011635027558 29.589712186160437 21.865686874872424 20 19.101857521943252 29.361094100836905 30.002041232904674 21.535007144315166 21 19.020208205756276 28.912022861808534 30.173504796897326 21.894264135537863 22 18.926311492141252 32.16983057766891 29.450908348642578 19.452949581547255 23 19.14676464584609 29.046744233517046 30.173504796897326 21.63298632373954 24 21.922841396203307 29.267197387221884 30.206164523372113 18.603796693202696 25 19.718309859154928 31.255358236374768 30.181669728516024 18.844662175954277 26 21.490100020412328 29.51622780159216 29.822412737293323 19.171259440702183 27 18.61196162482139 32.49642784241682 29.859154929577464 19.032455603184324 28 18.934476423759953 29.77342314758114 29.156970810369465 22.135129618289447 29 19.073280261277812 29.48765054092672 29.0181669728516 22.420902224943866 30 18.27311696264544 29.532557664829557 30.05103082261686 22.143294549908145 31 19.26107368850786 28.826291079812204 32.59848948765054 19.314145744029393 32 21.681975913451726 29.740763421106347 29.69585629720351 18.881404368238417 33 21.38803837517861 29.569299857113695 30.283731373749745 18.75893039395795 34 19.175341906511534 32.0514390691978 30.508266993263934 18.26495203102674 35 18.885486834047764 30.100020412329048 32.504592774035515 18.509899979587672 36 18.783425188814043 32.26780975709328 30.12043274137579 18.82833231271688 37 18.84057971014493 31.78607879159012 29.86731986119616 19.50602163706879 38 18.460910389875483 29.0181669728516 32.94549908144519 19.57542355582772 39 19.00387834251888 28.573178199632576 30.63482343335375 21.788120024494795 40 19.04470300061237 28.279240661359463 33.51296182894468 19.163094509083486 41 19.220249030414372 28.27107572974076 30.149010002041233 22.359665237803632 42 22.139212084098794 28.27515819555011 30.238824249846907 19.346805470504187 43 19.028373137374974 28.20167381098183 32.74137579097775 20.028577260665443 44 18.665033680342926 31.630945090834867 29.88364972443356 19.82037150438865 45 18.632373953868136 28.601755460298016 32.47601551337008 20.28985507246377 46 21.302306593182283 28.556848336395184 30.087773014900996 20.053072055521532 47 18.669116146152277 28.6752398448663 29.70810369463156 22.94754031434987 48 18.509899979587672 28.907940395999184 29.067156562563788 23.515003061849356 49 21.249234537660747 28.75688916105328 29.920391916717698 20.073484384568278 50 18.73035313329251 29.230455194937743 31.279853031230864 20.759338640538886 51 18.50581751377832 29.091651357419885 29.165135741988163 23.237395386813635 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 965.0 1 515.0 2 65.0 3 61.0 4 57.0 5 62.5 6 68.0 7 46.0 8 24.0 9 41.0 10 58.0 11 118.5 12 179.0 13 284.0 14 389.0 15 451.5 16 514.0 17 414.5 18 315.0 19 283.5 20 252.0 21 248.0 22 244.0 23 225.5 24 207.0 25 251.0 26 314.5 27 334.0 28 364.0 29 394.0 30 504.0 31 614.0 32 712.5 33 811.0 34 948.5 35 1086.0 36 1164.5 37 1243.0 38 1290.0 39 1337.0 40 1370.5 41 1404.0 42 1425.0 43 1446.0 44 1443.5 45 1441.0 46 1486.5 47 1532.0 48 1517.5 49 1503.0 50 1519.0 51 1535.0 52 1755.5 53 1976.0 54 1514.5 55 1053.0 56 954.0 57 855.0 58 769.0 59 683.0 60 597.5 61 512.0 62 452.0 63 392.0 64 317.5 65 243.0 66 208.0 67 173.0 68 145.0 69 117.0 70 96.5 71 76.0 72 55.5 73 35.0 74 31.0 75 22.5 76 18.0 77 13.0 78 8.0 79 9.0 80 10.0 81 6.0 82 2.0 83 2.5 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04490712390283731 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.008164931618697692 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 24495.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.79367920675868 #Duplication Level Percentage of deduplicated Percentage of total 1 96.81407202117691 83.06035437684885 2 2.731970571139301 4.687716135652611 3 0.18316802185245676 0.4714397552323872 4 0.08436906224447391 0.289533290447098 5 0.04209747646086736 0.18058486954488645 6 0.02022883337394962 0.10413036247269647 7 0.0 0.0 8 0.019452416411051988 0.1335115498692866 9 0.009636380553012957 0.07440664876914974 >10 0.05705380963384497 0.9739411791848757 >50 0.014231777682815857 0.9401673581768475 >100 0.014231777682791477 2.5600228064610397 >500 0.009487851788527651 6.524191667340297 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 931 3.8007756685037766 No Hit CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC 672 2.743417023882425 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 331 1.3512961828944683 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 179 0.7307613798734436 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 119 0.48581343131251276 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 94 0.38375178607879157 No Hit TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT 81 0.3306797305572566 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56 0.2286180853235354 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.14696876913655849 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.13063890589916308 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31 0.12655644008981423 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.10614411104307002 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.004082465809348847 23 0.0 0.0 0.016329863237395388 24 0.0 0.0 0.028577260665441927 25 0.0 0.0 0.028577260665441927 26 0.0 0.0 0.04490712390283731 27 0.0 0.0 0.04490712390283731 28 0.0 0.0 0.05307205552153501 29 0.0 0.0 0.07756685037762809 30 0.0 0.0 0.10206164523372117 31 0.0 0.0 0.2694427434170239 32 0.0 0.0 0.43274137579097777 33 0.0 0.0 0.6164523372116758 34 0.0 0.0 0.8328230251071648 35 0.0 0.0 0.9879567258624209 36 0.0 0.0 1.2737293325168402 37 0.0 0.0 1.7064707083078179 38 0.0 0.0 2.2208614002857727 39 0.0 0.0 2.7352520922637273 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTCT 30 2.0838943E-6 44.985683 45 GTATCAA 115 0.0 33.250286 1 CAACGCA 125 0.0 30.65295 5 TATCAAC 125 0.0 30.590263 2 TCAACGC 130 0.0 29.413717 4 AACGCAG 135 0.0 28.38236 6 ATCAACG 140 0.0 27.312738 3 TGCCGTC 70 8.723928E-7 25.706106 45 ACGCAGA 150 0.0 25.544125 7 CGCAGAG 150 0.0 25.544125 8 AGAGTAC 155 0.0 24.720123 11 AATCTCG 75 1.5917412E-6 23.992365 36 ATCTCGT 75 1.5917412E-6 23.992365 37 CAGAGTA 160 0.0 23.947618 10 GCAGAGT 165 0.0 23.221933 9 ACATCTC 80 2.7405604E-6 22.538937 8 TCTCCGA 80 2.7405604E-6 22.538937 11 GTATGCC 70 2.5723475E-5 22.492844 42 GCCCACG 80 2.7901242E-6 22.492844 18 TATGCCG 70 2.5723475E-5 22.492844 43 >>END_MODULE