##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_9.341000000068a6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13451 Sequences flagged as poor quality 0 Sequence length 51 %GC 23 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.39989591851907 38.0 38.0 38.0 32.0 38.0 2 36.36175749014943 38.0 38.0 38.0 32.0 38.0 3 36.3509776224816 38.0 38.0 38.0 32.0 38.0 4 36.331945580254256 38.0 38.0 38.0 32.0 38.0 5 36.36978663296409 38.0 38.0 38.0 32.0 38.0 6 38.35930414095606 40.0 38.0 40.0 38.0 40.0 7 38.22429559140584 40.0 38.0 40.0 38.0 40.0 8 38.17731023715709 40.0 38.0 40.0 38.0 40.0 9 38.10750130101851 40.0 38.0 40.0 38.0 40.0 10 38.09976953386365 40.0 38.0 40.0 32.0 40.0 11 37.93450301092856 40.0 38.0 40.0 32.0 40.0 12 37.90558322801279 40.0 38.0 40.0 32.0 40.0 13 37.91324065125269 40.0 38.0 40.0 32.0 40.0 14 37.707233662924686 40.0 38.0 40.0 32.0 40.0 15 37.701806557133295 40.0 38.0 40.0 32.0 40.0 16 37.755928927217305 40.0 38.0 40.0 32.0 40.0 17 37.81339677347409 40.0 38.0 40.0 32.0 40.0 18 37.86521448219463 40.0 38.0 40.0 32.0 40.0 19 37.94543156642629 40.0 38.0 40.0 32.0 40.0 20 38.0261690580626 40.0 38.0 40.0 32.0 40.0 21 38.01628131737417 40.0 38.0 40.0 32.0 40.0 22 37.983123931306224 40.0 38.0 40.0 32.0 40.0 23 37.93182662999033 40.0 38.0 40.0 32.0 40.0 24 37.854137238867 40.0 38.0 40.0 32.0 40.0 25 37.86789086313285 40.0 38.0 40.0 32.0 40.0 26 37.83688945059847 40.0 38.0 40.0 32.0 40.0 27 37.72574529774738 40.0 38.0 40.0 32.0 40.0 28 37.748940599211956 40.0 38.0 40.0 32.0 40.0 29 37.71853393799717 40.0 38.0 40.0 32.0 40.0 30 37.65177310237157 40.0 38.0 40.0 32.0 40.0 31 37.57817262657051 40.0 38.0 40.0 32.0 40.0 32 37.53906772730652 40.0 38.0 40.0 32.0 40.0 33 37.413500854955025 40.0 38.0 40.0 32.0 40.0 34 37.43914950561297 40.0 38.0 40.0 32.0 40.0 35 37.341312913538026 40.0 38.0 40.0 32.0 40.0 36 37.23923871831091 40.0 38.0 40.0 32.0 40.0 37 37.203627983049586 40.0 38.0 40.0 32.0 40.0 38 37.145862761133 40.0 38.0 40.0 32.0 40.0 39 37.03412385696231 40.0 38.0 40.0 32.0 40.0 40 36.928629841647464 40.0 38.0 40.0 32.0 40.0 41 36.906995762396846 40.0 38.0 40.0 32.0 40.0 42 36.77629915991376 40.0 38.0 40.0 27.0 40.0 43 36.722028101999854 40.0 38.0 40.0 27.0 40.0 44 36.613039922682326 40.0 38.0 40.0 27.0 40.0 45 36.460188833543974 40.0 38.0 40.0 27.0 40.0 46 36.26421827373429 40.0 38.0 40.0 27.0 40.0 47 36.19492974500037 40.0 38.0 40.0 27.0 40.0 48 36.117166009962084 40.0 38.0 40.0 27.0 40.0 49 36.0395509627537 40.0 38.0 40.0 27.0 40.0 50 35.86937774143186 40.0 38.0 40.0 27.0 40.0 51 35.86320719649097 40.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 -1.806639656623517 2201 2 -0.4497842793083464 2201 3 0.07929218636324009 2201 4 -0.422062703584146 2201 5 0.23736066452292448 2201 6 1.2387868635825612 2201 7 -1.718710363489734 2201 8 0.7118959412537507 2201 9 -3.2035185536284274 2201 10 0.06374433470726615 2201 11 -0.6913215891485152 2201 12 1.0587830594236394 2201 13 0.7677576022244637 2201 14 -1.6534154775661918 2201 15 -1.1933717448130778 2201 16 -0.8481421702643033 2201 17 -0.33720013730063414 2201 18 -0.4112613368169207 2201 19 0.3497938033747303 2201 20 -2.123129018409145 2201 21 -1.0688142462949273 2201 22 -1.1663810773210912 2201 23 0.3257455896830237 2201 24 -0.25340954298185636 2201 25 -0.13534452166604893 2201 26 -0.907446854309562 2201 27 -0.6252369599433081 2201 28 -0.3586281739546635 2201 29 -1.6120183819884488 2201 30 -1.1062843188831337 2201 31 0.9594122475280926 2201 32 0.5564921889813874 2201 33 -1.0599026263631686 2201 34 -1.272987031975127 2201 35 -0.1066407943651555 2201 36 -0.6048560303300619 2201 37 -0.5780908307742791 2201 38 -1.3821056526739852 2201 39 -0.439208537173144 2201 40 -0.16082375726658427 2201 41 0.5802699536540956 2201 42 0.5924906635320397 2201 43 -0.03835184761606314 2201 44 0.4302011910449153 2201 45 1.0789016738234025 2201 46 1.2298550429629458 2201 47 -1.149778385110487 2201 48 1.5135230380992724 2201 49 0.9464833716980507 2201 50 1.6906918506166093 2201 51 -0.20052708125060548 1101 1 1.1559271882962676 1101 2 0.5047611752371068 1101 3 0.40014780133650163 1101 4 0.5458517349185286 1101 5 0.5127617340416464 1101 6 0.5917280400531482 1101 7 -0.07967293033465239 1101 8 -0.6009382833451795 1101 9 0.5959466870132815 1101 10 0.9059903240120732 1101 11 1.1375554161990777 1101 12 -0.4358693469934778 1101 13 0.48968273591430744 1101 14 0.9882957523803313 1101 15 0.17293841561473045 1101 16 -0.1796929724033447 1101 17 -0.15270815869100574 1101 18 -0.4273041175655834 1101 19 0.9059435359950498 1101 20 0.9223255270454018 1101 21 0.24936757188689285 1101 22 0.11436758578051354 1101 23 -0.7544683140602899 1101 24 -1.8229282595593972 1101 25 -1.7503177836981294 1101 26 -0.5037035388015383 1101 27 -0.06106583694865719 1101 28 -2.0297511686070706 1101 29 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15 1.551726294402613 2202 16 1.5374787447683715 2202 17 1.1856756796928352 2202 18 1.451483761222299 2202 19 0.7746304046819219 2202 20 0.8768709815908551 2202 21 1.3756301981495156 2202 22 1.5133574847704097 2202 23 1.5283599687679938 2202 24 1.151819215188084 2202 25 1.7143286809483342 2202 26 1.4912459561479565 2202 27 1.4793382034553844 2202 28 1.510652871796971 2202 29 1.4925567814102436 2202 30 1.1878333281756923 2202 31 1.1489547311882262 2202 32 1.4388451301578584 2202 33 1.9204895304995802 2202 34 0.21394107260003636 2202 35 0.38028731021000794 2202 36 1.1369740350294109 2202 37 0.3859614568074221 2202 38 0.6276982688946475 2202 39 0.4660202209967963 2202 40 -0.9712812736064507 2202 41 0.4364791039808935 2202 42 1.788569094904588 2202 43 0.31785730271073476 2202 44 0.5968678577115796 2202 45 0.8338036346077118 2202 46 0.9814890298910512 2202 47 0.5168882815561773 2202 48 1.526594933524109 2202 49 1.3876598422862827 2202 50 3.050168974799611 2202 51 2.2667931801873067 1102 1 1.0953211276902053 1102 2 0.8835490540249893 1102 3 1.4910568922455951 1102 4 0.30342749249428635 1102 5 1.3157920370719438 1102 6 1.3695058178309267 1102 7 1.3597210090592853 1102 8 1.3132031307962322 1102 9 1.136350727417323 1102 10 1.3251822432039972 1102 11 0.6224039010475622 1102 12 0.8823124711883423 1102 13 0.7775615237930964 1102 14 0.8367806008651755 1102 15 0.16283740551372006 1102 16 0.37081207810170724 1102 17 0.8523423463594995 1102 18 1.007039316777849 1102 19 1.2190748491263648 1102 20 0.7657598704797408 1102 21 -0.5688142462949273 1102 22 -1.4866425152295903 1102 23 -2.1383066978986776 1102 24 -1.0704030070341446 1102 25 -1.7301157634961086 1102 26 -1.8976429327409292 1102 27 -2.3539951298779513 1102 28 -0.3782360170919148 1102 29 -1.5074432185897564 1102 30 -0.5899444496020863 1102 31 -1.2954897132562166 1102 32 1.4944006857134156 1102 33 0.42048953049958016 1102 34 0.8806077392667007 1102 35 -1.4530460231233278 1102 36 0.692529590584968 1102 37 0.27485034569630784 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50 0.3279467525773896 1212 51 0.25290429129841385 1213 1 0.7086544610235421 1213 2 -0.6497842793083493 1213 3 0.051056892245597396 1213 4 0.9167608258276232 1213 5 -0.7908746295947182 1213 6 0.07172804005314504 1213 7 0.5730543423926235 1213 8 -0.42457464698154723 1213 9 -0.04587149480489927 1213 10 -0.750373312351563 1213 11 -0.764262765619101 1213 12 -0.15768752881165682 1213 13 -2.255771809540242 1213 14 -0.3098860658014857 1213 15 0.3783929610692738 1213 16 -0.42696569967607445 1213 17 -0.6054354314182788 1213 18 0.5981504278889602 1213 19 0.6368526269041439 1213 20 0.4768709815908565 1213 21 0.491185753705075 1213 22 -0.21108695967403435 1213 23 0.3728044132124353 1213 24 0.44070810407696825 1213 25 -0.6812268746072263 1213 26 -0.6109762660742675 1213 27 0.2971159812331621 1213 28 1.0084306495747484 1213 29 0.5503345591880233 1213 30 0.8278333281756929 1213 31 1.0911769534104465 1213 32 1.3988451301578593 1213 33 0.14715619716624673 1213 34 0.011718850377818057 1213 35 1.018065087987786 1213 36 1.2680851461405283 1213 37 0.6548503456963104 1213 38 -1.4856350644386893 1213 39 -1.3450908901143208 1213 40 -1.1090590513842287 1213 41 -1.167965340463546 1213 42 -0.8380975717620771 1213 43 0.49341285826628933 1213 44 0.8302011910449139 1213 45 1.307136967941048 1213 46 0.9192668076688264 1213 47 1.8902216148895121 1213 48 1.388817155746331 1213 49 1.4076598422862858 1213 50 1.5212800859107247 1213 51 -0.30876237536825357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 1.0 17 3.0 18 5.0 19 4.0 20 6.0 21 7.0 22 15.0 23 37.0 24 40.0 25 72.0 26 96.0 27 100.0 28 150.0 29 178.0 30 237.0 31 291.0 32 350.0 33 407.0 34 553.0 35 678.0 36 845.0 37 1082.0 38 1828.0 39 6464.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.22159428911362 30.34651992861392 13.33283759666865 16.099048185603806 2 8.824622704631626 31.93814586276113 34.78551780536763 24.45171362723961 3 20.481748568879638 38.51014794439075 30.88246227046316 10.125641216266448 4 9.6424057690878 29.997769682551485 34.086685004832354 26.273139543528362 5 11.240799940524868 39.95241989443164 32.027358560701806 16.779421604341685 6 24.749089287041855 44.23462939558397 11.820682477139247 19.195598840234926 7 9.999256560850494 47.80313731321091 20.295888781503237 21.901717344435358 8 18.318340643818303 40.472827299085566 21.72329194855401 19.485540108542114 9 18.71236339305628 30.272842167868557 23.002007285703666 28.012787153371498 10 11.634822689762842 40.23492677124377 24.39967288677422 23.730577652219164 11 17.522860753847297 35.69251356776448 22.912794587762992 23.871831090625232 12 19.11382053378931 38.257378633558844 28.101999851312172 14.526800981339678 13 21.240056501375363 31.276485019701138 31.40286967511709 16.08058880380641 14 18.48189725670954 32.48829083339529 30.82298713850271 18.206824771392462 15 19.797784551334473 33.21686119991079 30.756077615047207 16.22927663370753 16 12.17753326890194 41.33521671251208 29.48479666939261 17.00245334919337 17 11.240799940524868 42.74031670507769 32.138874433127654 13.880008921269793 18 12.482343320199242 32.39907813545461 43.19381458627611 11.924763958070033 19 10.155378782246673 38.05665006319233 38.012043714221996 13.775927440339009 20 9.278120585830049 34.15359452828786 39.967288677421756 16.600996208460337 21 6.125938591926251 40.517433648055906 39.30562783436176 14.050999925656086 22 6.928852873392313 42.22734369191882 40.041632592372316 10.802170842316556 23 8.869229053601963 35.92297970411122 41.37982306148242 13.827968180804401 24 11.144152851089139 38.153297152628056 42.27195004088916 8.430599955393651 25 8.4900750873541 42.39090030481005 40.68099026094714 8.438034346888708 26 12.318786707308007 37.476767526577945 43.00052040740465 7.20392535870939 27 7.523604192996804 42.91874210095904 42.621366441156795 6.936287264887368 28 8.229871385027135 36.75563155155751 42.6362352241469 12.378261839268456 29 6.8693777414318635 37.77414318638019 48.293807151884614 7.062671920303323 30 6.0441602854806336 42.49498178574084 43.62500929298937 7.835848635789161 31 7.843283027284217 37.73697122890491 41.9597055980968 12.460040145714073 32 6.096201025946026 38.16816593561817 47.936956360121926 7.79867667831388 33 7.2857036651550064 39.075161698015016 46.68054419745744 6.9585904393725375 34 10.363541744108245 39.76656010705524 42.96334844992937 6.906549698907144 35 6.653780388075237 40.814809307858155 42.01918073005724 10.512229574009368 36 6.519961341164225 44.093375957177905 42.30168760686938 7.084975094788491 37 6.438183034718609 44.10824474016802 42.502416177235894 6.951156047877481 38 6.177979332391644 39.9078135454613 46.420340495130475 7.49386662701658 39 6.378707902758158 40.49513047357074 41.78128020221545 11.344881421455653 40 6.668649171065348 39.83346963051074 46.22704631625901 7.270834882164895 41 6.809902609471415 40.19032042227344 41.35751988699725 11.642257081257899 42 10.772433276336333 40.435655341610286 42.54702252620623 6.244888855847149 43 5.880603672589398 40.39104899263995 45.96684261393205 7.7615047208386 44 6.014422719500409 45.46873838376329 41.69950189576983 6.817337000966471 45 6.3638391197680475 41.06757861869006 44.83681510668352 7.7317671548583755 46 10.400713701583525 41.51364210839343 39.83346963051074 8.252174559512305 47 6.653780388075237 40.94119396327411 39.86320719649097 12.541818452159692 48 6.809902609471415 41.23856962307635 39.26845587688648 12.683071890565758 49 10.73526131886105 41.35751988699725 39.16437439595569 8.742844398186008 50 6.4084454687383845 41.99687755557208 43.19381458627611 8.400862389413428 51 6.111524163568773 41.49442379182156 39.59851301115241 12.79553903345725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1400.0 1 757.5 2 115.0 3 215.0 4 315.0 5 285.0 6 255.0 7 381.0 8 507.0 9 424.0 10 341.0 11 506.0 12 671.0 13 903.5 14 1136.0 15 1221.5 16 1307.0 17 979.5 18 652.0 19 520.0 20 388.0 21 505.5 22 623.0 23 464.5 24 306.0 25 273.5 26 367.5 27 494.0 28 372.0 29 250.0 30 291.5 31 333.0 32 280.5 33 228.0 34 229.5 35 231.0 36 253.5 37 276.0 38 304.5 39 333.0 40 299.0 41 265.0 42 285.5 43 306.0 44 330.5 45 355.0 46 342.0 47 329.0 48 350.5 49 372.0 50 597.5 51 823.0 52 503.5 53 184.0 54 165.0 55 146.0 56 119.0 57 92.0 58 77.5 59 63.0 60 53.0 61 43.0 62 37.5 63 32.0 64 22.5 65 13.0 66 11.0 67 9.0 68 8.5 69 8.0 70 5.5 71 3.0 72 2.5 73 2.0 74 2.0 75 1.0 76 0.0 77 0.5 78 1.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02230317448516839 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.007434391495056129 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 13451.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.521373875548285 #Duplication Level Percentage of deduplicated Percentage of total 1 92.56984347136913 34.73347706490223 2 3.308896374083614 2.4830867593487476 3 1.0303150386368138 1.159765073228756 4 0.5745987715474539 0.8623894134265111 5 0.2179512581731722 0.40889153222808716 6 0.17832375668714087 0.40145714073303096 7 0.059441252229046955 0.15612222139617873 8 0.19813750743015654 0.5947513196044905 9 0.0792550029720626 0.2676380938220207 >10 1.2284525460669704 9.984387777860382 >50 0.2179512581731722 5.427105791390974 >100 0.27739251040221913 23.49267712437737 >500 0.0396275014860313 10.088469258791168 >1k 0.01981375074301565 9.939781428890045 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1337 9.939781428890045 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 809 6.014422719500409 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 548 4.074046539290759 TruSeq Adapter, Index 12 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 460 3.4198200877258196 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 438 3.256263474834585 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 332 2.468217976358635 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 296 2.2005798825366143 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 226 1.6801724778826854 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 224 1.665303694892573 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 190 1.4125343840606648 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 185 1.3753624265853839 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 184 1.3679280350903278 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 154 1.144896290238644 No Hit GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 132 0.981339677347409 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 124 0.92186454538696 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 111 0.8252174559512304 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 104 0.7731767154858374 No Hit GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 80 0.5947513196044905 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 80 0.5947513196044905 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 78 0.579882536614378 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 74 0.5501449706341536 No Hit TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT 72 0.5352761876440414 TruSeq Adapter, Index 12 (95% over 22bp) AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 69 0.5129730131588729 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.43862909820831164 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.4237603152181994 No Hit GCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTT 56 0.41632592372314325 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 54 0.40145714073303096 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51 0.37915396624786263 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50 0.3717195747528065 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48 0.35685079176269424 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45 0.33454761727752585 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.3271132257824697 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.3271132257824697 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 43 0.31967883428741356 No Hit CTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.289941268307189 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 36 0.2676380938220207 No Hit TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 35 0.26020370232696455 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.25276931083190846 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.25276931083190846 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.23790052784179613 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.23790052784179613 No Hit ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACT 31 0.23046613634674 No Hit GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30 0.2230317448516839 No Hit ATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT 28 0.20816296186157163 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA 27 0.20072857036651548 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27 0.20072857036651548 No Hit TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 26 0.19329417887145936 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23 0.17099100438629097 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.17099100438629097 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23 0.17099100438629097 No Hit GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACAC 23 0.17099100438629097 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCCTGTCTCTTATACAC 22 0.16355661289123485 No Hit CTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT 21 0.15612222139617873 No Hit TGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT 20 0.14868782990112261 No Hit GATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT 20 0.14868782990112261 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.14125343840606647 No Hit GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACAT 19 0.14125343840606647 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.14125343840606647 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.14125343840606647 No Hit TTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.13381904691101035 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 18 0.13381904691101035 No Hit TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT 17 0.12638465541595423 RNA PCR Primer, Index 12 (95% over 23bp) ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA 17 0.12638465541595423 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17 0.12638465541595423 No Hit ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17 0.12638465541595423 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.11895026392089807 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAA 16 0.11895026392089807 No Hit AGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.11151587242584195 No Hit CTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 15 0.11151587242584195 No Hit GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA 14 0.10408148093078581 No Hit CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.10408148093078581 No Hit AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.10408148093078581 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.01486878299011226 14 0.0 0.0 0.03717195747528065 15 0.0 0.0 0.03717195747528065 16 0.0 0.0 0.05204074046539291 17 0.0 0.0 0.05947513196044904 18 0.0 0.0 0.06690952345550517 19 0.0 0.0 0.09664708943572968 20 0.0 0.0 0.15612222139617873 21 0.0 0.0 0.16355661289123485 22 0.0 0.0 0.19329417887145936 23 0.0 0.0 0.23790052784179616 24 0.0 0.0 0.2973756598022452 25 0.0 0.0 0.34198200877258195 26 0.0 0.0 0.3865883577429187 27 0.0 0.0 0.4088915322280871 28 0.0 0.0 0.4758010556835923 29 0.0 0.0 0.5129730131588729 30 0.0 0.0 0.6021857110995466 31 0.0 0.0 0.802914281466062 32 0.0 0.0 0.9962084603375214 33 0.0 0.0 1.1523306817337 34 0.0 0.0 1.3753624265853839 35 0.0 0.0 1.769385175823359 36 0.0 0.0 1.9775481376849304 37 0.0 0.0 2.5351274998141404 38 0.0 0.0 2.7804624191509926 39 0.0 0.0 3.0704036874581817 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGCC 80 0.0 42.1875 42 TTGCAAT 80 0.0 42.1875 32 AATCTCG 80 0.0 42.1875 36 ATCTCGT 80 0.0 42.1875 37 GCCCACG 80 0.0 42.1875 18 TGCAATC 80 0.0 42.1875 33 TGCCGTC 80 0.0 42.1875 45 ACTTGCA 80 0.0 42.1875 30 CCACGAG 80 0.0 42.1875 20 TATGCCG 80 0.0 42.1875 43 CAATCTC 80 0.0 42.1875 35 CACGAGA 80 0.0 42.1875 21 CCCACGA 80 0.0 42.1875 19 GCAATCT 80 0.0 42.1875 34 TCTCGTA 80 0.0 42.1875 38 ACGAGAC 80 0.0 42.1875 22 ATGCCGT 80 0.0 42.1875 44 CGTATGC 80 0.0 42.1875 41 TCGTATG 80 0.0 42.1875 40 CGAGACT 80 0.0 42.1875 23 >>END_MODULE