FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_88.34100000006d94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_88.34100000006d94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41377
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC21575.213041061459265No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT2450.5921163931652851RNA PCR Primer, Index 44 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC1720.41568987601807766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.17159291393769485No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT570.13775769147110714No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC560.13534088986635087No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.11842327863305702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.11600647702830075No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC3300.036.81719242
TATGCCG3300.036.81719243
ATGCCGT3300.036.81719244
CGTATGC3300.036.81719241
TCGTATG3300.036.81719240
CTCGTAT3250.036.69132239
CGAGCCC3350.036.26768515
ATCTCGT3300.036.13539537
GCCCACG3300.036.13539518
CCACGAG3300.036.13539520
CCCACGA3300.036.13539519
TGCCGTC3400.035.73433345
ACGAGAC3400.035.73433322
TCTCCGA3400.035.73433311
CTCCGAG3400.035.73433312
TATCTCG3150.035.71332636
GAGCCCA3350.035.5960616
CACGAGA3350.035.5960621
TCTCGTA3350.035.5960638
ATATCTC3100.035.5635635