##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_88.34100000006d94.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41377 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2741861420596 38.0 38.0 38.0 32.0 38.0 2 36.324358943374335 38.0 38.0 38.0 32.0 38.0 3 36.261763781811155 38.0 38.0 38.0 32.0 38.0 4 36.38854919399667 38.0 38.0 38.0 32.0 38.0 5 36.42035430311526 38.0 38.0 38.0 32.0 38.0 6 38.346037653769 40.0 38.0 40.0 38.0 40.0 7 38.22768687918409 40.0 38.0 40.0 38.0 40.0 8 38.16013727433115 40.0 38.0 40.0 38.0 40.0 9 38.22386833264857 40.0 38.0 40.0 38.0 40.0 10 38.16871692002803 40.0 38.0 40.0 38.0 40.0 11 38.09739710467168 40.0 38.0 40.0 38.0 40.0 12 38.05921163931653 40.0 38.0 40.0 38.0 40.0 13 38.09792880102472 40.0 38.0 40.0 38.0 40.0 14 38.051912898470164 40.0 38.0 40.0 32.0 40.0 15 38.074026633153686 40.0 38.0 40.0 38.0 40.0 16 38.07815936389782 40.0 38.0 40.0 38.0 40.0 17 38.04185900379438 40.0 38.0 40.0 32.0 40.0 18 38.045725886361986 40.0 38.0 40.0 32.0 40.0 19 38.07743432341639 40.0 38.0 40.0 32.0 40.0 20 38.05292795514416 40.0 38.0 40.0 32.0 40.0 21 38.09945138603572 40.0 38.0 40.0 38.0 40.0 22 38.11271962684583 40.0 38.0 40.0 38.0 40.0 23 38.07446165744254 40.0 38.0 40.0 38.0 40.0 24 38.04294656451652 40.0 38.0 40.0 32.0 40.0 25 38.033351862145636 40.0 38.0 40.0 32.0 40.0 26 37.96527056093965 40.0 38.0 40.0 32.0 40.0 27 37.96621311356551 40.0 38.0 40.0 32.0 40.0 28 37.94687870072746 40.0 38.0 40.0 32.0 40.0 29 37.91809459361481 40.0 38.0 40.0 32.0 40.0 30 37.94158590521304 40.0 38.0 40.0 32.0 40.0 31 37.93950745583295 40.0 38.0 40.0 32.0 40.0 32 37.91335766246949 40.0 38.0 40.0 32.0 40.0 33 37.87548638132296 40.0 38.0 40.0 32.0 40.0 34 37.87140198661092 40.0 38.0 40.0 32.0 40.0 35 37.859462986683425 40.0 38.0 40.0 32.0 40.0 36 37.81103028252411 40.0 38.0 40.0 32.0 40.0 37 37.794306015419195 40.0 38.0 40.0 32.0 40.0 38 37.79875293037195 40.0 38.0 40.0 32.0 40.0 39 37.671750006042004 40.0 38.0 40.0 32.0 40.0 40 37.70241921840636 40.0 38.0 40.0 32.0 40.0 41 37.69657055852285 40.0 38.0 40.0 32.0 40.0 42 37.6658530101264 40.0 38.0 40.0 32.0 40.0 43 37.6886676172753 40.0 38.0 40.0 32.0 40.0 44 37.60664137080987 40.0 38.0 40.0 32.0 40.0 45 37.581482466104355 40.0 38.0 40.0 32.0 40.0 46 37.45853976847041 40.0 38.0 40.0 32.0 40.0 47 37.43093989414409 40.0 38.0 40.0 32.0 40.0 48 37.3574691253595 40.0 38.0 40.0 32.0 40.0 49 37.281025690601055 40.0 38.0 40.0 32.0 40.0 50 37.241027624042346 40.0 38.0 40.0 32.0 40.0 51 36.78256035962008 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.008288859611099042 2201 2 -0.03200807569010777 2201 3 0.5438041702036429 2201 4 0.4887305297340063 2201 5 0.03286812551096574 2201 6 -0.18001228734357966 2201 7 -0.08173126771234251 2201 8 -0.10682731897586706 2201 9 0.2553649684296033 2201 10 0.3812759765687801 2201 11 0.8241542526412857 2201 12 0.6890851094740782 2201 13 0.4464365896470568 2201 14 -0.22316289917257137 2201 15 0.4203379742611304 2201 16 -0.5637143047426676 2201 17 0.38112551230967995 2201 18 -0.3059089993517645 2201 19 0.38907589344498916 2201 20 0.28396219765908626 2201 21 0.1581301763624836 2201 22 0.19147966025173702 2201 23 -0.7779972764631253 2201 24 0.8424397649022026 2201 25 -0.5788823077811571 2201 26 0.14098875031542946 2201 27 0.43020382859898376 2201 28 0.2162637373628442 2201 29 0.20977978122650853 2201 30 -0.0700534565968951 2201 31 -0.2518933189844077 2201 32 0.002730648175827355 2201 33 0.12068362656511766 2201 34 0.08442595553741228 2201 35 0.1297246700598933 2201 36 0.3811239987011348 2201 37 -0.23544950628978967 2201 38 -0.08084204635670034 2201 39 0.6651365361258712 2201 40 -0.28036114935567014 2201 41 0.10328074982565028 2201 42 0.5964755720659838 2201 43 0.46273111853010107 2201 44 0.275685663443916 2201 45 0.1595311872012033 2201 46 0.46164456332237336 2201 47 0.3739073460676323 2201 48 0.40949299980700005 2201 49 -0.3014670128698711 2201 50 0.18344652637626524 2201 51 -0.42516455461795744 1101 1 -1.1294304386345146 1101 2 -0.6469203563918668 1101 3 -0.4675993385682844 1101 4 -0.22179578605546624 1101 5 -0.08204415519078623 1101 6 0.3041982389722051 1101 7 -0.06945056595795762 1101 8 0.25106741786623843 1101 9 0.2808035649208307 1101 10 0.1426794853407074 1101 11 -0.053915922797308724 1101 12 -0.8863534870171534 1101 13 -0.8237388489494322 1101 14 -0.8608821974181851 1101 15 -0.32703044679150395 1101 16 -0.23476693632161982 1101 17 0.6513009509061689 1101 18 -0.40678619233422353 1101 19 -0.09776621181816836 1101 20 0.09624289941347541 1101 21 0.312516141274763 1101 22 -1.4216782344851069 1101 23 0.06235360072985685 1101 24 -0.6689637438697318 1101 25 -0.2516893253250174 1101 26 -0.6905901970529911 1101 27 -0.49172599596241895 1101 28 -0.015315210005574897 1101 29 -0.9217991661419092 1101 30 -0.45952714080741686 1101 31 -0.4299634944230064 1101 32 -1.2762167202452233 1101 33 0.03384152130195872 1101 34 -1.156802114638026 1101 35 -0.6369419966067724 1101 36 -2.042560211825176 1101 37 -1.8512389799739992 1101 38 -0.9536490639005635 1101 39 -1.040126621768863 1101 40 -0.3461506230398754 1101 41 -0.32917539052522216 1101 42 -2.2158051296884054 1101 43 -1.33376010954008 1101 44 -2.6041388979595936 1101 45 -0.37292495314967056 1101 46 -1.0594080682565732 1101 47 -1.367320724107806 1101 48 -0.08962980721054237 1101 49 0.17046281169152877 1101 50 0.05449915795521321 1101 51 -0.15148034409163813 2202 1 0.7856698119920509 2202 2 0.8120395433575069 2202 3 -0.7573863059868344 2202 4 -1.2196028035993294 2202 5 0.22810622074906206 2202 6 0.3164162840849869 2202 7 0.5242211132400385 2202 8 0.4384107762622307 2202 9 -0.08511122204658506 2202 10 -0.12110497581217317 2202 11 0.9122494907365208 2202 12 0.7057517761407439 2202 13 0.21667468488515595 2202 14 0.5899323389226652 2202 15 0.6500998790230312 2202 16 0.692238076209712 2202 17 0.23588741707158078 2202 18 -0.14519471363747982 2202 19 0.6331235124926096 2202 20 0.5470574357543256 2202 21 0.5343206525529638 2202 22 0.3414796602517356 2202 23 -0.07799727646312249 2202 24 0.7138683363307692 2202 25 1.3711176922188457 2202 26 1.012417321743996 2202 27 0.9825847809799342 2202 28 0.9853113564104632 2202 29 1.1145416859884136 2202 30 0.8180417814983443 2202 31 0.6850114429203558 2202 32 0.4693973148424959 2202 33 0.961159817041306 2202 34 0.947521193632646 2202 35 1.0297246700598919 2202 36 -0.34863790606076606 2202 37 0.9347885889483081 2202 38 0.9727293822147303 2202 39 0.4877555837449208 2202 40 0.23154361254909617 2202 41 -0.6979097263648271 2202 42 0.7559993815897954 2202 43 -0.8979831671841865 2202 44 -0.17431433655608686 2202 45 -0.08094500327498366 2202 46 0.20093027760808724 2202 47 -0.18561646345617788 2202 48 -0.20836414305014728 2202 49 0.28543774903489094 2202 50 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-0.9376497067682124 1213 34 -0.2905740444625877 1213 35 -0.8036086632734438 1213 36 -0.19387600129886096 1213 37 -0.46600506184534396 1213 38 -1.2613976019122575 1213 39 -1.948752352763016 1213 40 0.38630551731100127 1213 41 0.0977251942700974 1213 42 0.16869779428820664 1213 43 -0.15393554813656607 1213 44 -0.2715365587783083 1213 45 -0.015468812798793863 1213 46 0.12553345221125767 1213 47 0.22112956828985375 1213 48 0.4206041109181129 1213 49 -0.30980034620320396 1213 50 0.11955763748738235 1213 51 -0.11127566572906744 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 2.0 18 0.0 19 0.0 20 1.0 21 9.0 22 8.0 23 22.0 24 62.0 25 118.0 26 163.0 27 260.0 28 361.0 29 469.0 30 606.0 31 712.0 32 868.0 33 1118.0 34 1435.0 35 1709.0 36 2334.0 37 3532.0 38 6424.0 39 21163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.565574761271606 17.962045207300857 13.209234860389218 31.26314517103832 2 14.104795050270688 20.485305491105954 43.18445475638051 22.225444702242847 3 18.62628996785654 22.640597433356696 33.32286052637939 25.410252072407374 4 13.659762670082412 22.282910795852768 33.6926311719071 30.364695362157722 5 14.406554365952099 34.093820238296644 37.48700969137443 14.012615704376827 6 29.429393141117043 40.1744930758634 16.47775334122822 13.918360441791332 7 26.13770935543901 30.944727747299222 19.334412838050124 23.58315005921164 8 22.56084298039974 40.25424752882036 19.365831258911957 17.819078231867948 9 22.783188728037317 18.739879643280084 19.626845832225634 38.850085796456966 10 15.20168209391691 32.87091862628997 30.27285690117698 21.65454237861614 11 30.471034632766997 22.971699253208307 28.585929381057106 17.97133673296759 12 21.7173792203398 23.882833458201418 28.380501244652827 26.019286076805955 13 25.308746405007614 21.11076201754598 31.11873746284168 22.46175411460473 14 19.46008652149745 21.258186915436113 25.908113202987167 33.37361336007927 15 21.246102907412332 28.784107112647124 22.848442371365735 27.121347608574816 16 26.474284609435422 28.120166279969066 25.120843000773398 20.28470610982212 17 20.330135099209706 33.371196558474516 26.28030065011963 20.018367692196147 18 25.834441087613293 26.124471299093656 27.630211480362536 20.410876132930515 19 20.388138337723856 26.18362858592938 26.795079391932713 26.633153684414047 20 20.438891171423737 25.992701259153637 27.16001643425091 26.408391135171716 21 20.008700485777123 26.22713101481499 27.024675544384564 26.73949295502332 22 20.37363752809532 32.46247915508616 26.476061580104886 20.687821736713634 23 20.65156971264229 26.176378181115112 26.81199700316601 26.36005510307659 24 26.647654494042584 25.707518669792396 27.0778451796892 20.566981656475818 25 21.197766875317207 31.739855475264033 27.184184450298478 19.878193199120282 26 25.719602677816177 26.76124416946613 27.474200642869228 20.044952509848464 27 19.832273968629917 32.2836358363342 26.957005099451386 20.927085095584502 28 19.767020325301495 26.73949295502332 26.60415206515697 26.88933465451821 29 20.74824177683254 32.44556154385286 26.282717451724384 20.523479227590208 30 20.22767921883308 31.635809928940883 26.792188330835792 21.344322521390243 31 20.59840007733765 26.48814558812867 26.505063199361967 26.408391135171716 32 19.71385068999686 31.706020252797444 27.677211977668755 20.90291707953694 33 20.19962781255287 27.05609396524639 31.865529158711357 20.878749063489376 34 20.25038064625275 31.123571066051188 27.62887594557363 20.997172342122436 35 19.62926263383039 31.68426903835464 27.756966430625713 20.929501897189258 36 20.00386688256761 26.13770935543901 33.19718684293206 20.661236919061317 37 19.97003166010102 30.35744495734345 27.800468859511323 21.872054523044206 38 19.740435507649178 24.648959566909152 33.54520627401696 22.065398651424704 39 20.368803924885807 23.469560383788096 28.622181405128455 27.539454286197646 40 20.363970321676295 23.539647630326026 34.00923218213017 22.087149865867513 41 20.557314450056797 23.00795127727965 28.859027962394567 27.575706310268988 42 25.395751262778838 23.165043381588806 29.796746985039995 21.642458370592358 43 19.370664862121465 23.45989317736907 34.71977185392851 22.449670106580953 44 18.4571138555236 29.571984435797667 29.066872900403606 22.90402880827513 45 19.034729439060346 23.3366362955265 35.0412064673611 22.587427798052058 46 24.40002900161926 23.12395775430795 28.646349421176016 23.82966382289678 47 18.52236749885202 23.716074147473236 28.35391642700051 29.407641926674238 48 18.619039563042268 23.69190613142567 28.689851850061626 28.99920245547043 49 23.981922323996425 23.832080624501533 29.14662735336056 23.039369698141478 50 17.11095536167436 24.70212920221379 34.26782995383909 23.91908548227276 51 16.84510718515117 24.472533049761942 28.46267249921454 30.21968726587235 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 50.0 1 27.5 2 5.0 3 8.0 4 11.0 5 13.5 6 16.0 7 15.5 8 15.0 9 24.0 10 33.0 11 46.5 12 60.0 13 110.0 14 160.0 15 178.0 16 196.0 17 211.0 18 226.0 19 193.5 20 161.0 21 153.0 22 145.0 23 141.0 24 137.0 25 123.0 26 121.0 27 133.0 28 150.5 29 168.0 30 336.5 31 505.0 32 922.5 33 1340.0 34 2070.5 35 2801.0 36 3399.0 37 3997.0 38 4127.0 39 4257.0 40 3835.0 41 3413.0 42 2949.5 43 2486.0 44 2540.5 45 2595.0 46 3554.0 47 4513.0 48 3310.5 49 2108.0 50 2074.0 51 2040.0 52 2020.0 53 2000.0 54 1909.0 55 1818.0 56 1658.0 57 1498.0 58 1366.5 59 1235.0 60 1139.5 61 1044.0 62 864.5 63 685.0 64 606.0 65 527.0 66 429.0 67 331.0 68 266.5 69 202.0 70 172.0 71 142.0 72 124.5 73 107.0 74 77.5 75 41.5 76 35.0 77 25.0 78 15.0 79 10.0 80 5.0 81 4.5 82 4.0 83 2.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029001619257075187 2 0.0024168016047562656 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0024168016047562656 17 0.0 18 0.004833603209512531 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.007250404814268797 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 41377.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.42211830659885 #Duplication Level Percentage of deduplicated Percentage of total 1 96.30412368221597 85.15414617642227 2 2.9914556011982465 5.290216821561782 3 0.4029617339821345 1.0689219034560151 4 0.1338967869196732 0.47357750135539195 5 0.047246152883739845 0.208880245990885 6 0.025214830938598838 0.13377292586002051 7 0.02025250757204791 0.12535387343786364 8 0.01286279830843682 0.09098846990260161 9 0.0030999386685461538 0.02466928293240496 >10 0.04249493963855004 0.7673917744168299 >50 0.008195518134805416 0.4444612100009384 >100 0.00546367302617318 1.0072833965479686 >500 0.0 0.0 >1k 0.00273183651308659 5.210336418115031 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 2157 5.213041061459265 No Hit TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT 245 0.5921163931652851 RNA PCR Primer, Index 44 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 172 0.41568987601807766 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71 0.17159291393769485 No Hit TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT 57 0.13775769147110714 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 56 0.13534088986635087 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.11842327863305702 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48 0.11600647702830075 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0024168016047562656 3 0.0 0.0 0.0024168016047562656 4 0.0 0.0 0.0024168016047562656 5 0.0 0.0 0.0024168016047562656 6 0.0 0.0 0.0024168016047562656 7 0.0 0.0 0.0024168016047562656 8 0.0 0.0 0.0024168016047562656 9 0.0 0.0 0.0024168016047562656 10 0.0 0.0 0.0024168016047562656 11 0.0 0.0 0.0024168016047562656 12 0.0 0.0 0.0024168016047562656 13 0.0 0.0 0.0024168016047562656 14 0.0 0.0 0.0024168016047562656 15 0.0 0.0 0.0024168016047562656 16 0.0 0.0 0.009667206419025062 17 0.0 0.0 0.012084008023781328 18 0.0 0.0 0.012084008023781328 19 0.0 0.0 0.012084008023781328 20 0.0 0.0 0.012084008023781328 21 0.0 0.0 0.014500809628537593 22 0.0 0.0 0.019334412838050125 23 0.0 0.0 0.03141842086183145 24 0.0 0.0 0.04833603209512531 25 0.0 0.0 0.04833603209512531 26 0.0 0.0 0.0628368417236629 27 0.0 0.0 0.08700485777122556 28 0.0 0.0 0.11117287381878821 29 0.0 0.0 0.19092732677574498 30 0.0 0.0 0.26101457331367667 31 0.0 0.0 0.6307852188413853 32 0.0 0.0 1.1987335959591077 33 0.0 0.0 1.793266790729149 34 0.0 0.0 2.5352248833893225 35 0.0 0.0 3.2650989680257148 36 0.0 0.0 4.195567585856877 37 0.0 0.0 5.416052396258791 38 0.0 0.0 6.8516325494840125 39 0.0 0.0 8.248543877033134 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGCC 330 0.0 36.817192 42 TATGCCG 330 0.0 36.817192 43 ATGCCGT 330 0.0 36.817192 44 CGTATGC 330 0.0 36.817192 41 TCGTATG 330 0.0 36.817192 40 CTCGTAT 325 0.0 36.691322 39 CGAGCCC 335 0.0 36.267685 15 ATCTCGT 330 0.0 36.135395 37 GCCCACG 330 0.0 36.135395 18 CCACGAG 330 0.0 36.135395 20 CCCACGA 330 0.0 36.135395 19 TGCCGTC 340 0.0 35.734333 45 ACGAGAC 340 0.0 35.734333 22 TCTCCGA 340 0.0 35.734333 11 CTCCGAG 340 0.0 35.734333 12 TATCTCG 315 0.0 35.713326 36 GAGCCCA 335 0.0 35.59606 16 CACGAGA 335 0.0 35.59606 21 TCTCGTA 335 0.0 35.59606 38 ATATCTC 310 0.0 35.56356 35 >>END_MODULE