##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_87.34100000006d87.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16538 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.296589672269924 38.0 38.0 38.0 32.0 38.0 2 36.40397871568509 38.0 38.0 38.0 32.0 38.0 3 36.395876163985974 38.0 38.0 38.0 32.0 38.0 4 36.499939533196276 38.0 38.0 38.0 32.0 38.0 5 36.58580239448543 38.0 38.0 38.0 32.0 38.0 6 38.57903011246825 40.0 38.0 40.0 38.0 40.0 7 38.303966622324346 40.0 38.0 40.0 38.0 40.0 8 38.30596202684726 40.0 38.0 40.0 38.0 40.0 9 38.443100737695005 40.0 38.0 40.0 38.0 40.0 10 38.275728624984886 40.0 38.0 40.0 38.0 40.0 11 38.340488571774095 40.0 38.0 40.0 38.0 40.0 12 38.05091304873624 40.0 38.0 40.0 38.0 40.0 13 38.254625710484945 40.0 38.0 40.0 38.0 40.0 14 38.152497278993835 40.0 38.0 40.0 38.0 40.0 15 38.21483855363405 40.0 38.0 40.0 38.0 40.0 16 37.960998911597535 40.0 38.0 40.0 32.0 40.0 17 37.92792356996009 40.0 38.0 40.0 32.0 40.0 18 37.89557382996735 40.0 38.0 40.0 32.0 40.0 19 38.04921997823195 40.0 38.0 40.0 32.0 40.0 20 38.09323981134357 40.0 38.0 40.0 32.0 40.0 21 38.211875680251545 40.0 38.0 40.0 38.0 40.0 22 38.252086104728505 40.0 38.0 40.0 38.0 40.0 23 38.22245737090337 40.0 38.0 40.0 38.0 40.0 24 38.07878824525336 40.0 38.0 40.0 32.0 40.0 25 37.91782561373806 40.0 38.0 40.0 32.0 40.0 26 37.906397387834076 40.0 38.0 40.0 32.0 40.0 27 37.914076671907125 40.0 38.0 40.0 32.0 40.0 28 37.97303180553876 40.0 38.0 40.0 32.0 40.0 29 38.01529810134236 40.0 38.0 40.0 38.0 40.0 30 38.11428225903979 40.0 38.0 40.0 38.0 40.0 31 38.056536461482644 40.0 38.0 40.0 38.0 40.0 32 38.02630305962027 40.0 38.0 40.0 32.0 40.0 33 37.991776514693434 40.0 38.0 40.0 38.0 40.0 34 37.978715685088886 40.0 38.0 40.0 32.0 40.0 35 37.940561131938566 40.0 38.0 40.0 32.0 40.0 36 38.01106542508163 40.0 38.0 40.0 38.0 40.0 37 37.76853307534164 40.0 38.0 40.0 32.0 40.0 38 37.784012577095176 40.0 38.0 40.0 32.0 40.0 39 37.54680130608296 40.0 38.0 40.0 32.0 40.0 40 37.52176804934091 40.0 38.0 40.0 32.0 40.0 41 37.701777724029505 40.0 38.0 40.0 32.0 40.0 42 37.74241141613254 40.0 38.0 40.0 32.0 40.0 43 37.911839400169306 40.0 38.0 40.0 32.0 40.0 44 37.743923086225664 40.0 38.0 40.0 32.0 40.0 45 37.75547224573709 40.0 38.0 40.0 32.0 40.0 46 37.45271495948724 40.0 38.0 40.0 32.0 40.0 47 37.492078848712055 40.0 38.0 40.0 32.0 40.0 48 37.438626194219374 40.0 38.0 40.0 32.0 40.0 49 37.26931914379006 40.0 38.0 40.0 32.0 40.0 50 37.14886927077035 40.0 38.0 40.0 32.0 40.0 51 36.777421695489174 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.375016258467042 2201 2 0.8295145956021415 2201 3 -0.2474669739218598 2201 4 -0.7067229151034269 2201 5 -0.6167454735759961 2201 6 -1.9412271772534169 2201 7 0.012987987353298536 2201 8 -0.353235363492459 2201 9 -1.548937127596048 2201 10 -1.3660282391261234 2201 11 -1.695757916791095 2201 12 -2.1297578847507026 2201 13 -0.4327649818142163 2201 14 -0.335365204208955 2201 15 -0.3506998965309478 2201 16 -0.2687878604850198 2201 17 -1.7814929435628954 2201 18 0.36151957760748843 2201 19 -0.7540129788723888 2201 20 -2.3899222828310513 2201 21 -0.8407690547234523 2201 22 -1.6615851076169648 2201 23 -1.663365574114117 2201 24 -0.45070209886489465 2201 25 -0.7062642432366673 2201 26 -0.6285215952457079 2201 27 0.217803355388007 2201 28 -0.9528790258589197 2201 29 -0.222949760806884 2201 30 -0.8970798389485424 2201 31 -1.9469553155702215 2201 32 -1.0932379304761994 2201 33 -0.9977789438095499 2201 34 -0.5309427973479828 2201 35 -3.5434174576604605 2201 36 -1.284997673018161 2201 37 0.26045159087815506 2201 38 -0.1209046658596904 2201 39 1.062414123488992 2201 40 1.4241583346032272 2201 41 0.1126726574292789 2201 42 0.44033906873841033 2201 43 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25 -0.3299201572151631 1213 26 -1.777715143632804 1213 27 -1.0510138489130725 1213 28 -2.5200833269341913 1213 29 -2.263272341452044 1213 30 -2.456219623894782 1213 31 -0.5101273585809736 1213 32 -0.5865175003686716 1213 33 -0.08648862122890222 1213 34 -1.423415915627551 1213 35 -1.3431486404561568 1213 36 -1.698976167641817 1213 37 -2.896806473637973 1213 38 -2.3641842357521625 1213 39 -4.968499855005632 1213 40 -3.4656266116333327 1213 41 -2.4908219662266404 1213 42 -2.2841232968529894 1213 43 -1.034138487559403 1213 44 -0.6405905598444335 1213 45 0.016583710840606614 1213 46 -0.8742103930575453 1213 47 -1.7441875044106112 1213 48 -0.7521618453375751 1213 49 -1.1845080484387154 1213 50 -0.51576754037999 1213 51 -2.2736246910973676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 2.0 21 1.0 22 7.0 23 17.0 24 20.0 25 42.0 26 56.0 27 97.0 28 128.0 29 181.0 30 238.0 31 300.0 32 358.0 33 413.0 34 526.0 35 634.0 36 921.0 37 1411.0 38 2588.0 39 8597.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.513758693680074 12.81524039915331 10.22074387662534 53.45025703054127 2 9.118394001693071 15.159027693796107 60.63006409481196 15.092514209698876 3 12.800822348530657 17.087918732615794 53.41032773007619 16.700931188777364 4 9.596081751118636 47.647841335107024 23.739267142338853 19.01680977143548 5 9.29374773249486 24.470915467408393 57.03833595356149 9.197000846535254 6 18.774942556536462 59.11234732132059 12.075220703833596 10.037489418309347 7 15.96323618333535 22.415044140766717 14.820413592937479 46.80130608296045 8 13.943644938928529 59.24537428951505 13.532470673600194 13.278510097956223 9 14.20969887531745 14.584593058410933 13.478050550247914 57.7276575160237 10 10.581690651832144 53.85778207763937 21.65920909420728 13.901318176321201 11 19.379610593784015 16.144636594509613 50.65304148022736 13.822711331479018 12 14.227838916434877 17.28141250453501 20.377312855242472 48.11343572378764 13 17.34792598863224 15.267867940500665 51.89865763695731 15.485548433909784 14 12.075220703833596 16.156729955254566 18.400048373442978 53.368000967468866 15 14.905067118152134 20.66150683274882 16.519530777603094 47.913895271495946 16 47.581327851009796 20.77034707945338 18.055387592211876 13.592937477324949 17 13.925504897811102 54.95827790542992 18.25492804450357 12.861289152255411 18 47.85632218661184 19.108665416943822 19.671040696619702 13.363971699824637 19 13.871084774458822 19.62752448905551 17.771193614705528 48.730197121780144 20 13.230136654976418 18.907969524730923 18.3456282500907 49.51626557020196 21 12.740355544805901 19.585197726448182 17.90422058289999 49.77022614584593 22 12.945942677470068 54.359656548554845 18.327488208973275 14.366912565001813 23 13.943644938928529 18.696335711694278 17.970734066997217 49.38928528237997 24 47.62365461361712 20.226145845930585 19.04704317329786 13.103156367154432 25 15.292054661990568 53.42242109082114 18.27911476599347 13.006409481194826 26 48.077155641552785 20.9638408513726 18.57540210424477 12.383601402829846 27 13.012456161567298 54.24476962147781 18.80517595839884 13.937598258556052 28 12.710122142943526 20.93360744951022 18.71447575281171 47.64179465473455 29 14.06457854637804 53.58568146087798 18.448421816422783 13.901318176321201 30 12.608090947572112 52.77861764528028 19.465441132007015 15.147850275140593 31 13.816664651106542 19.669851251662838 18.690289031321804 47.82319506590881 32 12.510581690651831 53.05962026847261 20.818720522433185 13.611077518442375 33 12.691982101826097 20.601040029024066 53.49498125529084 13.211996613858993 34 12.855242471882935 54.33546982706494 19.83915830209215 12.97012939895997 35 14.312492441649535 51.49957673237393 20.625226750513967 13.56270407546257 36 12.129640827185876 19.053089853670336 54.71641069053091 14.100858628612892 37 11.845446849679526 51.49353005200145 21.368968436328455 15.292054661990568 38 11.754746644092394 16.45906397387834 55.48433909783529 16.30185028419398 39 12.4440682065546 15.787882452533559 22.445277542629096 49.322771798282744 40 12.286854516870239 15.18321441528601 56.1736606602975 16.35627040754626 41 13.387350344660781 14.651106542508163 22.10666344177047 49.85487967106059 42 46.22687144757528 15.533921876889586 23.442979804087553 14.796226871447576 43 11.694279840367638 17.311645906397388 55.9015600435361 15.092514209698876 44 10.648204135929374 51.275849558592334 22.929011972427137 15.146934333051155 45 11.59753295440803 17.39025275123957 56.209940742532346 14.80227355182005 46 44.84218164227839 16.75535131212964 21.604788970855 16.797678074736968 47 10.926351433063248 16.495344056113193 21.520135445640342 51.058169065183215 48 10.503083806989963 17.505139678316603 20.413592937477326 51.57818357721611 49 44.26170032652074 17.039545289635992 21.375015116700933 17.323739267142336 50 9.632361833353489 17.208852340065302 54.67408392792357 18.484701898657637 51 8.858386745676624 17.529326399806504 21.151287942919335 52.460998911597535 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 94.0 1 50.5 2 7.0 3 6.5 4 6.0 5 5.5 6 5.0 7 4.5 8 4.0 9 6.0 10 8.0 11 17.5 12 27.0 13 33.5 14 40.0 15 65.5 16 91.0 17 76.5 18 62.0 19 53.0 20 44.0 21 35.0 22 26.0 23 30.5 24 35.0 25 31.0 26 30.0 27 33.0 28 31.5 29 30.0 30 60.5 31 91.0 32 147.0 33 203.0 34 321.5 35 440.0 36 686.5 37 933.0 38 1173.0 39 1413.0 40 1537.5 41 1662.0 42 1568.5 43 1475.0 44 1412.5 45 1350.0 46 3764.5 47 6179.0 48 3332.0 49 485.0 50 534.0 51 583.0 52 442.0 53 301.0 54 280.5 55 260.0 56 211.5 57 163.0 58 146.0 59 129.0 60 115.5 61 102.0 62 94.5 63 87.0 64 72.0 65 57.0 66 41.5 67 26.0 68 23.5 69 21.0 70 17.0 71 13.0 72 10.5 73 8.0 74 8.0 75 7.0 76 6.0 77 4.0 78 2.0 79 1.5 80 1.0 81 0.5 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018140041117426533 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.006046680372475511 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.006046680372475511 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 16538.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.570462036791604 #Duplication Level Percentage of deduplicated Percentage of total 1 96.08571254855802 52.43441728909156 2 2.774160985911373 3.0277449353124988 3 0.4280987342288716 0.7008463717270554 4 0.20010488698274073 0.436792645538725 5 0.07493697077390678 0.20446725593848206 6 0.07491619574182647 0.24529268494021136 7 0.012485329131179463 0.047693112555891405 8 0.049940744781695065 0.2180231613758871 9 0.012485157159025916 0.06131887152930006 >10 0.2122475481747962 2.0303347779665475 >50 0.03745544927828406 1.6624217539680797 >100 0.024970299518856032 2.159785639376562 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.012485149759428016 36.7708615006792 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 5397 32.63393397025033 No Hit TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT 186 1.124682549280445 RNA PCR Primer, Index 44 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 131 0.7921151287942919 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.5079211512879429 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG 80 0.4837344297980409 No Hit TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT 80 0.4837344297980409 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48 0.2902406578788245 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 26 0.15721368968436328 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 24 0.14512032893941226 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 20 0.12093360744951022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.12093360744951022 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.10279356633208368 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.006046680372475511 2 0.0 0.0 0.006046680372475511 3 0.0 0.0 0.006046680372475511 4 0.0 0.0 0.006046680372475511 5 0.0 0.0 0.006046680372475511 6 0.0 0.0 0.006046680372475511 7 0.0 0.0 0.006046680372475511 8 0.0 0.0 0.012093360744951022 9 0.0 0.0 0.012093360744951022 10 0.0 0.0 0.012093360744951022 11 0.0 0.0 0.024186721489902044 12 0.0 0.0 0.030233401862377555 13 0.0 0.0 0.036280082234853066 14 0.0 0.0 0.04837344297980409 15 0.0 0.0 0.04837344297980409 16 0.0 0.0 0.054420123352279595 17 0.0 0.0 0.07256016446970613 18 0.0 0.0 0.10279356633208368 19 0.0 0.0 0.13302696819446125 20 0.0 0.0 0.1632603700568388 21 0.0 0.0 0.2660539363889225 22 0.0 0.0 0.30838069899625103 23 0.0 0.0 0.37489418309348166 24 0.0 0.0 0.580481315757649 25 0.0 0.0 0.5925746765026001 26 0.0 0.0 0.816301850284194 27 0.0 0.0 0.9009553754988512 28 0.0 0.0 0.9795622203410328 29 0.0 0.0 1.1851493530052002 30 0.0 0.0 1.4391099286491715 31 0.0 0.0 2.0921514088765267 32 0.0 0.0 2.9084532591607206 33 0.0 0.0 3.9122022009916555 34 0.0 0.0 5.26665860442617 35 0.0 0.0 6.445761277058895 36 0.0 0.0 7.884871205708066 37 0.0 0.0 9.499334865159028 38 0.0 0.0 11.210545410569598 39 0.0 0.0 12.788728987785706 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTCTT 25 3.7256796E-5 45.000004 27 GTATCTC 25 3.7256796E-5 45.000004 35 AGTATCT 25 3.7256796E-5 45.000004 34 AGACGTC 25 3.7256796E-5 45.000004 25 GACGTCT 25 3.7256796E-5 45.000004 26 GTCTTAG 25 3.7256796E-5 45.000004 29 CGTCTTA 25 3.7256796E-5 45.000004 28 GAGACGT 25 3.7256796E-5 45.000004 24 CGAGACG 30 2.0379048E-6 44.999996 23 TGCCGTC 595 0.0 41.97479 45 ATGCCGT 600 0.0 41.625 44 GTATGCC 605 0.0 41.280994 42 TATGCCG 605 0.0 41.280994 43 CGTATGC 605 0.0 41.280994 41 TCGTATG 605 0.0 41.280994 40 CTCGTAT 600 0.0 41.25 39 ATCTCGT 605 0.0 40.909092 37 TATCTCG 605 0.0 40.909092 36 ACATAAT 570 0.0 40.657894 30 ACAACAT 570 0.0 40.657894 27 >>END_MODULE