FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_87.34100000006d87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_87.34100000006d87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16538
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC539732.63393397025033No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT1861.124682549280445RNA PCR Primer, Index 44 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC1310.7921151287942919No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.5079211512879429No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG800.4837344297980409No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT800.4837344297980409No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.2902406578788245No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA260.15721368968436328No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC240.14512032893941226No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC200.12093360744951022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.12093360744951022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.10279356633208368No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTT253.7256796E-545.00000427
GTATCTC253.7256796E-545.00000435
AGTATCT253.7256796E-545.00000434
AGACGTC253.7256796E-545.00000425
GACGTCT253.7256796E-545.00000426
GTCTTAG253.7256796E-545.00000429
CGTCTTA253.7256796E-545.00000428
GAGACGT253.7256796E-545.00000424
CGAGACG302.0379048E-644.99999623
TGCCGTC5950.041.9747945
ATGCCGT6000.041.62544
GTATGCC6050.041.28099442
TATGCCG6050.041.28099443
CGTATGC6050.041.28099441
TCGTATG6050.041.28099440
CTCGTAT6000.041.2539
ATCTCGT6050.040.90909237
TATCTCG6050.040.90909236
ACATAAT5700.040.65789430
ACAACAT5700.040.65789427
Produced by FastQC (version 0.11.2)