##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_86.34100000006d7a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 67603 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.28745765720456 38.0 38.0 38.0 32.0 38.0 2 36.28032779610373 38.0 38.0 38.0 32.0 38.0 3 36.24265195331568 38.0 38.0 38.0 32.0 38.0 4 36.343816102835675 38.0 38.0 38.0 32.0 38.0 5 36.36127094951407 38.0 38.0 38.0 32.0 38.0 6 38.21521234264752 40.0 38.0 40.0 38.0 40.0 7 38.11571971658063 40.0 38.0 40.0 38.0 40.0 8 38.09901927429256 40.0 38.0 40.0 38.0 40.0 9 38.09592769551647 40.0 38.0 40.0 38.0 40.0 10 38.074878333801756 40.0 38.0 40.0 38.0 40.0 11 38.01674481901691 40.0 38.0 40.0 32.0 40.0 12 37.965164267857936 40.0 38.0 40.0 32.0 40.0 13 37.98359540257089 40.0 38.0 40.0 32.0 40.0 14 37.90416105794122 40.0 38.0 40.0 32.0 40.0 15 37.93597917252193 40.0 38.0 40.0 32.0 40.0 16 37.92133485200361 40.0 38.0 40.0 32.0 40.0 17 37.93623064065204 40.0 38.0 40.0 32.0 40.0 18 37.955371803026495 40.0 38.0 40.0 32.0 40.0 19 37.95443989172078 40.0 38.0 40.0 32.0 40.0 20 37.94404094492848 40.0 38.0 40.0 32.0 40.0 21 37.97056343653388 40.0 38.0 40.0 32.0 40.0 22 37.98091800659734 40.0 38.0 40.0 32.0 40.0 23 37.94421845184385 40.0 38.0 40.0 32.0 40.0 24 37.93859739952369 40.0 38.0 40.0 32.0 40.0 25 37.94646687277192 40.0 38.0 40.0 32.0 40.0 26 37.89303729124447 40.0 38.0 40.0 32.0 40.0 27 37.85141191958937 40.0 38.0 40.0 32.0 40.0 28 37.824208984808365 40.0 38.0 40.0 32.0 40.0 29 37.819445882579174 40.0 38.0 40.0 32.0 40.0 30 37.793441119476945 40.0 38.0 40.0 32.0 40.0 31 37.79230211676997 40.0 38.0 40.0 32.0 40.0 32 37.79634039909472 40.0 38.0 40.0 32.0 40.0 33 37.74566217475556 40.0 38.0 40.0 32.0 40.0 34 37.77171131458663 40.0 38.0 40.0 32.0 40.0 35 37.74628344895937 40.0 38.0 40.0 32.0 40.0 36 37.70027957339171 40.0 38.0 40.0 32.0 40.0 37 37.70695087496117 40.0 38.0 40.0 32.0 40.0 38 37.7195538659527 40.0 38.0 40.0 32.0 40.0 39 37.638048607310324 40.0 38.0 40.0 32.0 40.0 40 37.63145126695561 40.0 38.0 40.0 32.0 40.0 41 37.624350990340666 40.0 38.0 40.0 32.0 40.0 42 37.61217697439463 40.0 38.0 40.0 32.0 40.0 43 37.64566661242844 40.0 38.0 40.0 32.0 40.0 44 37.59779891424937 40.0 38.0 40.0 32.0 40.0 45 37.609913761223616 40.0 38.0 40.0 32.0 40.0 46 37.520287561202906 40.0 38.0 40.0 32.0 40.0 47 37.494445512773105 40.0 38.0 40.0 32.0 40.0 48 37.449772939070755 40.0 38.0 40.0 32.0 40.0 49 37.45493543185953 40.0 38.0 40.0 32.0 40.0 50 37.392452997648036 40.0 38.0 40.0 32.0 40.0 51 36.9167196722039 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.1454703524465799 2201 2 -0.20879829254295856 2201 3 -0.0633941275960197 2201 4 -0.23352358317578847 2201 5 -0.013183145199811008 2201 6 -0.1209651108920724 2201 7 0.4407907776454323 2201 8 0.5853229363185548 2201 9 0.6333311455178645 2201 10 0.3866387159337208 2201 11 0.10969093498714244 2201 12 0.13006827390180575 2201 13 -0.2893045323090391 2201 14 -0.12161799330654333 2201 15 0.5772100347019489 2201 16 0.683372972545456 2201 17 0.5865413629413965 2201 18 0.19375681990362636 2201 19 0.0882088815372768 2201 20 0.45051988147112354 2201 21 0.06973818792580033 2201 22 -0.20585483756466516 2201 23 0.4357591517722881 2201 24 0.5410272599965822 2201 25 0.6405621353739974 2201 26 0.5162028261071896 2201 27 -0.2599716314505187 2201 28 0.3372053060596514 2201 29 0.1459576818142736 2201 30 0.4373785665032699 2201 31 0.5924499420614993 2201 32 0.5646711959327817 2201 33 -0.3137292299373797 2201 34 0.21293942294013135 2201 35 0.04186906494787479 2201 36 0.24648586397899663 2201 37 0.09583373585109456 2201 38 0.8256537732763931 2201 39 0.2692894906373198 2201 40 0.32000231145502767 2201 41 0.2847828669427628 2201 42 0.5366463966952821 2201 43 0.4258799311828909 2201 44 0.8313027886454805 2201 45 -0.6945184565373879 2201 46 -0.14639155275638416 2201 47 -0.23939644559359863 2201 48 -0.5195136552248343 2201 49 -0.19242753516897437 2201 50 -0.2749904019183447 2201 51 -0.18954531546307152 1101 1 0.1365634698554885 1101 2 0.29415138935409146 1101 3 0.2994745826411105 1101 4 0.8465226863441586 1101 5 0.5212298102657797 1101 6 0.6093992615775647 1101 7 0.5842262895019985 1101 8 0.4701118316337656 1101 9 0.779427154771767 1101 10 0.7012714516190925 1101 11 7.262154961082956E-4 1101 12 0.6929369841389388 1101 13 0.23527615016638492 1101 14 0.8757215092961204 1101 15 0.34216087333699363 1101 16 0.43640940979242515 1101 17 0.5629439077649891 1101 18 0.09254224500484298 1101 19 0.3883823922370979 1101 20 0.28094209084070343 1101 21 0.21294235218259416 1101 22 0.3681185574613579 1101 23 0.06895753233909119 1101 24 -0.1677061118946881 1101 25 0.076421013338134 1101 26 0.1676776670557132 1101 27 0.7854881719040208 1101 28 0.3485413384483138 1101 29 1.12629313583394 1101 30 0.8377255879029235 1101 31 0.2467009542072489 1101 32 -0.10739358139515076 1101 33 0.2883528984605377 1101 34 0.15521819679784699 1101 35 -0.03308755737717206 1101 36 -0.44547480692904173 1101 37 -0.44783312360523553 1101 38 -0.5714543817264968 1101 39 -0.3646029327287934 1101 40 -0.2699340679550417 1101 41 0.03990143258764789 1101 42 0.15573257367619675 1101 43 0.6907729329179944 1101 44 0.8329222218438659 1101 45 0.46198819470610175 1101 46 0.995308852101914 1101 47 0.7215058100454996 1101 48 -0.14102898200331992 1101 49 -0.5019706236594317 1101 50 -0.13687588485645819 1101 51 -0.16837701008423522 2202 1 0.22314757718262968 2202 2 0.31468802506100246 2202 3 -0.08741912220924775 2202 4 0.26561453360938714 2202 5 -0.046365648970549955 2202 6 0.49969853918334195 2202 7 0.4748351646309956 2202 8 -0.013685897984395012 2202 9 0.14722901235690955 2202 10 0.5944603061082532 2202 11 0.041386690212313226 2202 12 0.655062887131713 2202 13 0.18419255883511454 2202 14 0.27107754644782034 2202 15 0.46538068757848094 2202 16 0.8817138473569202 2202 17 0.7025723907371315 2202 18 0.5595185091947315 2202 19 0.5555650547757978 2202 20 0.8152042167436946 2202 21 0.7649547360835243 2202 22 0.5534642746956209 2202 23 0.14088735690049248 2202 24 0.24292340306919158 2202 25 -0.00778951297765218 2202 26 0.400495004517019 2202 27 0.5010712472394161 2202 28 0.4005537223492439 2202 29 0.2769639304675877 2202 30 1.0289536580783647 2202 31 1.1014997158171553 2202 32 0.7992111657668701 2202 33 0.42199582931709045 2202 34 0.748923047159046 2202 35 0.4773355592379005 2202 36 0.881047380893456 2202 37 0.7523732747435758 2202 38 0.2619821507812432 2202 39 0.9300307102020682 2202 40 0.04430741811307115 2202 41 -0.47722963036385124 2202 42 0.769354864697867 2202 43 0.726892643960305 2202 44 0.12466628789133694 2202 45 0.3411419614759694 2202 46 -0.1388500746266743 2202 47 -0.26600708985130694 2202 48 0.33224243182536384 2202 49 0.25004176024150127 2202 50 -0.17124120993386072 2202 51 0.7004568392449713 1102 1 0.3043022612784938 1102 2 0.4214418376971665 1102 3 0.5269599627580703 1102 4 -0.2583506080028144 1102 5 -0.3949495269661938 1102 6 0.20667021674325525 1102 7 -0.18039359353893758 1102 8 0.03424438524000095 1102 9 0.001259513446228766 1102 10 0.18324026253527137 1102 11 0.20696403226023818 1102 12 0.11857051240404815 1102 13 0.03985704685254632 1102 14 0.35223223054368447 1102 15 0.35590356339547924 1102 16 0.32724761642010236 1102 17 0.10017587657591065 1102 18 0.24361436976117545 1102 19 0.5321445754729694 1102 20 0.21008439103563603 1102 21 -0.2606443924567827 1102 22 0.19453181282198528 1102 23 -0.6646681986550576 1102 24 -0.15250143353211598 1102 25 -0.19297469816283552 1102 26 0.4424340023383664 1102 27 0.5326616611827717 1102 28 0.4582879271422726 1102 29 0.49373952959612666 1102 30 -0.16549078636608527 1102 31 0.5475781471897037 1102 32 -0.007978376716792468 1102 33 0.19868428247612968 1102 34 -0.16719895719825217 1102 35 -0.1550173819385705 1102 36 0.8401977076908409 1102 37 0.18374582376318216 1102 38 0.801742499365119 1102 39 0.9180481393959639 1102 40 -0.1354311439784297 1102 41 -0.17004008788018865 1102 42 0.6103134703623567 1102 43 -0.13476312946017543 1102 44 -0.1950504877077961 1102 45 -0.3756336376525695 1102 46 0.19121528485045047 1102 47 0.3783284221312684 1102 48 -0.39161377732496305 1102 49 0.09481300207156096 1102 50 -0.48551136243930415 1102 51 -0.41827954419729707 2203 1 -0.727832814974235 2203 2 0.24606057408060877 2203 3 0.4027769562221266 2203 4 0.2067910041976191 2203 5 0.5124578804412181 2203 6 0.6369534411441293 2203 7 0.7493449685525633 2203 8 -0.16074472151380803 2203 9 -0.04884941901563877 2203 10 -0.5035789095780174 2203 11 0.1786415921730935 2203 12 -0.060623387378093696 2203 13 -0.4138466568511632 2203 14 0.17303833076154973 2203 15 0.5045963738529906 2203 16 0.8523020826510361 2203 17 0.3006116064234021 2203 18 -0.2640109025699786 2203 19 -0.11110161189086654 2203 20 0.1289297069397719 2203 21 0.3629939517697949 2203 22 0.03385643155836959 2203 23 0.3565736314102992 2203 24 0.21351163836331466 2203 25 0.09024970270862553 2203 26 0.36127931824250936 2203 27 0.44224771782765515 2203 28 0.1358478399963019 2203 29 -0.1544086185520257 2203 30 -0.4710463419216353 2203 31 -0.3102649900651926 2203 32 -0.2988280499194005 2203 33 -0.27408260205545787 2203 34 0.1999034393159107 2203 35 0.21262967688495849 2203 36 0.704576792658159 2203 37 0.4288438629788658 2203 38 0.5855115625459462 2203 39 0.7143444356922615 2203 40 -0.0635357191418322 2203 41 0.44433899708712943 2203 42 0.6222960411684539 2203 43 0.08963774199952468 2203 44 0.5462349153423176 2203 45 -0.04121097970050158 2203 46 -0.33492850599922264 2203 47 0.35163996897222916 2203 48 0.2734189024136029 2203 49 -0.16172294564085377 2203 50 0.05424898614457163 2203 51 -0.25052355291189343 1103 1 -0.2150580080817761 1103 2 0.3625192787746059 1103 3 -0.33667640087828943 1103 4 -0.5950509447031536 1103 5 -0.5734007054173702 1103 6 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27 -0.5666649381438233 2204 28 0.53067849953284 2204 29 -0.12410558824899454 2204 30 0.20572133484603938 2204 31 0.6662650158765757 2204 32 0.6889913202529101 2204 33 0.6296607134525587 2204 34 0.41529262529333266 2204 35 -0.5363305132517056 2204 36 -0.4754588579657266 2204 37 0.03223067224803344 2204 38 0.4170623646849876 2204 39 0.16383938518720953 2204 40 0.3595183509710651 2204 41 0.3703639525238458 2204 42 0.7550946151434985 2204 43 1.1261796314825858 2204 44 0.6525926099353043 2204 45 0.6361508741319426 2204 46 0.8326294262645959 2204 47 0.6451634389662857 2204 48 -0.1062602419714267 2204 49 0.20339886065742263 2204 50 0.25270412577618373 2204 51 -0.10851523443298561 2205 1 -0.047549590573360945 2205 2 -0.8470766808213526 2205 3 0.036219222017329855 2205 4 0.17683457718236895 2205 5 0.003198621181958572 2205 6 -0.4044408943678519 2205 7 0.09738418423884099 2205 8 -0.2713111703155562 2205 9 -0.6283701161833974 2205 10 -0.5852782559832477 2205 11 0.3365936618898715 2205 12 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1213 27 0.07895795747906931 1213 28 -0.7731935543392083 1213 29 -0.5062604704038733 1213 30 -0.2580833789586734 1213 31 -0.5450144454028845 1213 32 -0.7950154137538235 1213 33 0.18016576395761064 1213 34 -1.2134952534945498 1213 35 -1.0994618263830205 1213 36 -1.2338763663832353 1213 37 -0.8347726947553369 1213 38 0.08877953640215708 1213 39 -0.4430629717151433 1213 40 -0.7187644773117654 1213 41 -0.46633638417648626 1213 42 0.15660976665864723 1213 43 -1.0606890553860993 1213 44 -0.5469023395596437 1213 45 -0.5700780820970195 1213 46 -0.79952545589029 1213 47 -0.8901900963872507 1213 48 -0.9471693328805131 1213 49 -0.40518699792843904 1213 50 -0.2540298809578232 1213 51 -1.131242507160259 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 3.0 17 0.0 18 3.0 19 1.0 20 5.0 21 12.0 22 31.0 23 59.0 24 99.0 25 153.0 26 302.0 27 425.0 28 631.0 29 759.0 30 1008.0 31 1309.0 32 1500.0 33 1849.0 34 2282.0 35 2912.0 36 3890.0 37 5793.0 38 10958.0 39 33618.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.655622475102476 22.929399055891796 14.608520650517187 26.80645781848854 2 12.763124805846068 23.27184509104895 43.12953950385349 20.83549059925149 3 18.40746712424005 26.137893288759372 31.221987189917606 24.232652397082973 4 13.756785941452302 20.223954558229664 37.890330310785025 28.128929189533007 5 13.650281792228155 37.99683446000917 36.084197446858866 12.268686300903806 6 28.608197861041667 40.58991464875819 18.244752451814268 12.55713503838587 7 25.050663432096208 33.914175406416874 22.737156635060572 18.298004526426343 8 21.636613759744392 42.341316213777496 20.90587695812316 15.116193068354955 9 23.74598760410041 19.21660281348461 22.21203201041374 34.825377572001244 10 14.759700013313019 31.636169992455955 34.31948286318654 19.28464713104448 11 31.60214783367602 23.896868482167953 27.30500125734065 17.19598242681538 12 22.14250846855909 25.729627383400143 32.38317826131976 19.744685886721 13 27.49138351848291 23.602502847506766 27.855272694998746 21.050840939011582 14 18.694436637427334 24.88794875967043 27.839001227756167 28.578613375146073 15 20.610032099167196 35.248435720308265 23.379139978995013 20.76239220152952 16 20.036685847842488 31.299832842709428 29.703702607949587 18.959778701498497 17 19.250624972264543 32.429034214457936 29.29307870952472 19.027262103752793 18 20.437265169669537 28.92961746693885 32.56412532173604 18.06899204165557 19 21.14551129387749 28.0194665917193 28.935106430188007 21.8999156842152 20 20.990192742925608 29.40845820451755 29.507566232267802 20.093782820289043 21 19.468070943597176 29.35224768131592 27.936630031211635 23.24305134387527 22 20.596719080514177 32.426075765868376 27.79314527461799 19.184059878999452 23 19.493217756608434 30.430602192210404 29.523837699510374 20.55234235167078 24 22.117361655547832 29.275328017987366 29.95872964217564 18.648580684289158 25 20.20176619380797 30.56521160303537 29.75311746520125 19.479904737955415 26 20.01094625978137 30.332973388754937 30.196884753635196 19.4591955978285 27 19.74172743813144 30.603671434699642 29.442480363297484 20.212120763871425 28 19.45475792494416 29.42472967176013 28.911438841471533 22.20907356182418 29 21.56561099359496 29.764951259559485 27.624513705013094 21.044924041832463 30 19.484342410839755 30.28711743561676 28.68067985148588 21.5478603020576 31 21.676552815703445 28.69695131872846 27.957339171338553 21.669156694229546 32 19.682558466340254 31.164297442421194 28.784225552120468 20.368918539118084 33 19.6130349244856 29.383311391506297 31.445350058429362 19.558303625578745 34 20.175140156501932 29.615549605786722 31.02820880730145 19.181101430409893 35 20.15591024066979 29.75311746520125 29.819682558466344 20.271289735662616 36 19.096785645607444 29.08450808396077 31.10069079774566 20.718015472686126 37 19.74172743813144 28.541632767776576 28.580092599440853 23.136547194651126 38 20.151472567785454 28.84931142109078 29.513483129446914 21.48573288167685 39 21.33189355501975 26.57870212860376 29.340413886957677 22.748990429418814 40 21.8703311983196 26.319837877017292 31.55333343194829 20.25649749271482 41 21.252015443101637 25.500347617709274 30.779699125778443 22.467937813410646 42 21.037527920358563 27.273937547150272 30.94241379820422 20.74612073428694 43 18.596807833971866 27.3597325562475 32.495599307723026 21.5478603020576 44 18.244752451814268 28.028341937487983 30.906912415129504 22.819993195568244 45 18.663372927236956 26.593494371551557 31.970474683076194 22.77265801813529 46 19.67368312057157 25.716314364747127 30.793012144431458 23.81699037024984 47 19.99467479253879 25.808026271023476 30.1451119033179 24.052187033119832 48 17.466680472760086 26.913006819224 30.47941659393814 25.140896114077776 49 19.919234353505022 26.167477774654973 30.411372276378266 23.50191559546174 50 17.6708134254397 28.109699273700873 31.006020442879755 23.213466857979675 51 17.047337278106507 27.35059171597633 30.21301775147929 25.389053254437872 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 161.0 1 87.5 2 14.0 3 15.0 4 16.0 5 14.5 6 13.0 7 15.5 8 18.0 9 23.0 10 28.0 11 52.0 12 76.0 13 119.0 14 162.0 15 174.5 16 187.0 17 187.5 18 188.0 19 162.5 20 137.0 21 128.5 22 120.0 23 118.5 24 117.0 25 132.0 26 227.5 27 308.0 28 711.5 29 1115.0 30 2133.0 31 3151.0 32 4627.5 33 6104.0 34 7564.5 35 9025.0 36 9158.0 37 9291.0 38 8072.0 39 6853.0 40 5713.5 41 4574.0 42 3894.5 43 3215.0 44 3030.0 45 2845.0 46 3075.5 47 3306.0 48 2895.0 49 2484.0 50 2461.5 51 2439.0 52 2406.0 53 2373.0 54 2258.5 55 2144.0 56 1968.5 57 1793.0 58 1631.5 59 1470.0 60 1304.5 61 1139.0 62 987.0 63 835.0 64 740.0 65 645.0 66 515.5 67 386.0 68 332.0 69 278.0 70 208.5 71 139.0 72 135.0 73 131.0 74 109.5 75 61.5 76 35.0 77 32.5 78 30.0 79 21.0 80 12.0 81 10.5 82 9.0 83 5.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03845983166427525 2 0.002958448589559635 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.002958448589559635 17 0.0 18 0.0014792242947798174 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0044376728843394525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 67603.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.00903614119427 #Duplication Level Percentage of deduplicated Percentage of total 1 93.09354936028987 80.99980000801514 2 5.062548853237423 8.80974992275793 3 0.9605383252649348 2.507265415739368 4 0.28418941899143874 0.9890818971188438 5 0.17493683570223434 0.7610542730020936 6 0.12984146106361333 0.6778428226985641 7 0.06452928411583818 0.39302415696602494 8 0.03893360267575465 0.27100601938573077 9 0.0316674350893515 0.24798177037694777 >10 0.14394703324702166 2.161888312493569 >50 0.010212666544428494 0.6871644168646106 >100 0.003403815890075415 0.4087045795957012 >500 0.0017019078880074613 1.08543640498546 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 733 1.0842714080736062 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 152 0.22484209280653225 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 124 0.18342381255269735 No Hit TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT 94 0.13904708370930283 RNA PCR Primer, Index 44 (95% over 22bp) GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 91 0.1346094108249634 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.13313018653018358 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.002958448589559635 11 0.0 0.0 0.002958448589559635 12 0.0 0.0 0.002958448589559635 13 0.0 0.0 0.002958448589559635 14 0.0 0.0 0.002958448589559635 15 0.0 0.0 0.002958448589559635 16 0.0 0.0 0.002958448589559635 17 0.0 0.0 0.00591689717911927 18 0.0 0.0 0.00591689717911927 19 0.0 0.0 0.00591689717911927 20 0.0 0.0 0.00591689717911927 21 0.0 0.0 0.01183379435823854 22 0.0 0.0 0.026626037306036715 23 0.0 0.0 0.09023268198156886 24 0.0 0.0 0.13017173794062395 25 0.0 0.0 0.14792242947798176 26 0.0 0.0 0.1952576069109359 27 0.0 0.0 0.22484209280653225 28 0.0 0.0 0.26773959735514696 29 0.0 0.0 0.31803322337766077 30 0.0 0.0 0.42749582119136725 31 0.0 0.0 0.769196633285505 32 0.0 0.0 1.2662159963315238 33 0.0 0.0 1.789861396683579 34 0.0 0.0 2.3371743857521117 35 0.0 0.0 2.8667366832832863 36 0.0 0.0 3.6285371950948924 37 0.0 0.0 4.569323846574856 38 0.0 0.0 5.75862017957783 39 0.0 0.0 7.107672736417023 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACGAC 35 1.1895463E-7 44.99926 25 GAGACGA 35 1.1895463E-7 44.99926 24 CGAGACG 35 1.1895463E-7 44.99926 23 GACGACG 35 1.1895463E-7 44.99926 26 ACGACGC 35 1.1895463E-7 44.99926 27 TGCCGTC 100 0.0 40.499336 45 CGCTATA 40 3.3947254E-7 39.37435 31 ACGCTAT 40 3.3947254E-7 39.37435 30 GACGCTA 40 3.3947254E-7 39.37435 29 CTCGTAT 110 0.0 38.863 39 TCTCGTA 110 0.0 38.863 38 CGTATGC 105 0.0 38.570797 41 TATGCCG 105 0.0 38.570797 43 TATCTCG 105 0.0 38.570797 36 TCGTATG 105 0.0 38.570797 40 ACGAGAC 105 0.0 38.570797 22 ATCTCGT 115 0.0 37.173306 37 GCCCACG 110 0.0 36.817577 18 CACGAGA 110 0.0 36.817577 21 CCCACGA 110 0.0 36.817577 19 >>END_MODULE