FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_86.34100000006d7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_86.34100000006d7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67603
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC7331.0842714080736062No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.22484209280653225No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1240.18342381255269735No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT940.13904708370930283RNA PCR Primer, Index 44 (95% over 22bp)
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT910.1346094108249634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.13313018653018358No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAC351.1895463E-744.9992625
GAGACGA351.1895463E-744.9992624
CGAGACG351.1895463E-744.9992623
GACGACG351.1895463E-744.9992626
ACGACGC351.1895463E-744.9992627
TGCCGTC1000.040.49933645
CGCTATA403.3947254E-739.3743531
ACGCTAT403.3947254E-739.3743530
GACGCTA403.3947254E-739.3743529
CTCGTAT1100.038.86339
TCTCGTA1100.038.86338
CGTATGC1050.038.57079741
TATGCCG1050.038.57079743
TATCTCG1050.038.57079736
TCGTATG1050.038.57079740
ACGAGAC1050.038.57079722
ATCTCGT1150.037.17330637
GCCCACG1100.036.81757718
CACGAGA1100.036.81757721
CCCACGA1100.036.81757719