FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_85.34100000006d6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_85.34100000006d6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63054
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC14892.361467948107971No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT1890.2997430773622609RNA PCR Primer, Index 44 (95% over 22bp)
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT960.15225045199352938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.13797697211913598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.11101595457861516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT451.8813807E-839.9943645
TAATACA301.12866706E-437.4947134
CAACGCA250.002093519535.9949265
ACGCAGA250.002093519535.9949267
ATCAACG250.002093519535.9949263
AACGCAG250.002093519535.9949266
TCAACGC250.002093519535.9949264
TGCCGTC2250.035.99492345
ATGCCGT2200.035.7904144
CTCGTAT2300.035.2124339
TCTCGTA2300.035.2124338
TATGCCG2250.034.99506443
CCCACGA2400.034.6826119
CGTATGC2300.034.23430641
TCGTATG2300.034.23430640
AATATCT2250.033.99520534
ATCTCGT2450.033.974837
GCCCACG2450.033.974818
AGCCCAC2450.033.974817
CACGAGA2450.033.974821
Produced by FastQC (version 0.11.2)