FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_84.34100000006d51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_84.34100000006d51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45515
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC10042.2058661979567176No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT1010.2219048665275184RNA PCR Primer, Index 44 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAACA1450.043.44827326
ACAACAT1450.043.44827327
CAACATA1400.043.39285728
CACGAGA1400.043.39285721
ATAATAT1400.043.39285732
TAATATC1400.043.39285733
GAGACAA1400.043.39285724
TGCCGTC1450.041.8965545
TATGCCG1450.041.8965543
TCTCGTA1450.041.8965538
ACGAGAC1450.041.8965522
TATCTCG1450.041.8965536
ATGCCGT1450.041.8965544
TCGTATG1450.041.8965540
CGAGACA1450.041.8965523
CTCGTAT1450.041.8965539
CATAATA1450.041.8965531
GTATGCC1500.040.542
ATCTCGT1500.040.537
CCACGAG1500.040.520