##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H23NVBCXX l02n01 48h_83.34100000006d44.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6533
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.16087555487525	38.0	38.0	38.0	32.0	38.0
2	36.23373641512322	38.0	38.0	38.0	32.0	38.0
3	36.22286851369968	38.0	38.0	38.0	32.0	38.0
4	36.40961273534364	38.0	38.0	38.0	32.0	38.0
5	36.35466095208939	38.0	38.0	38.0	32.0	38.0
6	38.19745905403337	40.0	38.0	40.0	38.0	40.0
7	38.13592530231134	40.0	38.0	40.0	38.0	40.0
8	37.99204041022501	40.0	38.0	40.0	32.0	40.0
9	38.05234960967396	40.0	38.0	40.0	32.0	40.0
10	38.027399357110056	40.0	38.0	40.0	32.0	40.0
11	37.99831624062452	40.0	38.0	40.0	32.0	40.0
12	37.854125210469924	40.0	38.0	40.0	32.0	40.0
13	38.04729833154753	40.0	38.0	40.0	38.0	40.0
14	37.923465482932805	40.0	38.0	40.0	32.0	40.0
15	37.997091688351446	40.0	38.0	40.0	32.0	40.0
16	37.85045155365069	40.0	38.0	40.0	32.0	40.0
17	37.85856421245982	40.0	38.0	40.0	32.0	40.0
18	37.857645798255014	40.0	38.0	40.0	32.0	40.0
19	37.87739170365835	40.0	38.0	40.0	32.0	40.0
20	37.93907852441451	40.0	38.0	40.0	32.0	40.0
21	37.94520128577989	40.0	38.0	40.0	32.0	40.0
22	37.99418337670289	40.0	38.0	40.0	32.0	40.0
23	37.972600642889944	40.0	38.0	40.0	32.0	40.0
24	38.000153069034134	40.0	38.0	40.0	32.0	40.0
25	37.780805143119544	40.0	38.0	40.0	32.0	40.0
26	37.73274146640135	40.0	38.0	40.0	32.0	40.0
27	37.7062605234961	40.0	38.0	40.0	32.0	40.0
28	37.77361089851523	40.0	38.0	40.0	32.0	40.0
29	37.77361089851523	40.0	38.0	40.0	32.0	40.0
30	37.85596203887953	40.0	38.0	40.0	32.0	40.0
31	37.823817541711314	40.0	38.0	40.0	32.0	40.0
32	37.81432726159498	40.0	38.0	40.0	32.0	40.0
33	37.6929435175264	40.0	38.0	40.0	32.0	40.0
34	37.78310117863156	40.0	38.0	40.0	32.0	40.0
35	37.736415123220574	40.0	38.0	40.0	32.0	40.0
36	37.641512322057245	40.0	38.0	40.0	32.0	40.0
37	37.558855043624675	40.0	38.0	40.0	32.0	40.0
38	37.564824735955916	40.0	38.0	40.0	32.0	40.0
39	37.42905250267871	40.0	38.0	40.0	32.0	40.0
40	37.391550589315784	40.0	38.0	40.0	32.0	40.0
41	37.491198530537275	40.0	38.0	40.0	32.0	40.0
42	37.43670595438543	40.0	38.0	40.0	32.0	40.0
43	37.40716363079749	40.0	38.0	40.0	32.0	40.0
44	37.32312873105771	40.0	38.0	40.0	32.0	40.0
45	37.17082504209399	40.0	38.0	40.0	32.0	40.0
46	36.9808663707332	40.0	38.0	40.0	32.0	40.0
47	37.00780652074086	40.0	38.0	40.0	32.0	40.0
48	36.91290371957753	40.0	38.0	40.0	32.0	40.0
49	36.8749425991122	40.0	38.0	40.0	32.0	40.0
50	36.787999387723865	38.0	38.0	40.0	32.0	40.0
51	36.33736415123221	38.0	38.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2201	1	0.27922624250746964
2201	2	0.6897007072788384
2201	3	1.1243459231740403
2201	4	0.6291712627650057
2201	5	0.7328772356115962
2201	6	1.1169047098734595
2201	7	1.3307236600205457
2201	8	1.4554855300949043
2201	9	1.039501730907979
2201	10	1.2693691291347662
2201	11	1.2263377247752274
2201	12	0.9301494946026239
2201	13	-0.011287453865570285
2201	14	1.2294358852952598
2201	15	0.9628974757880968
2201	16	0.529609886641154
2201	17	-1.8580733936202662
2201	18	-0.0685908709346208
2201	19	0.5294872662060257
2201	20	-1.8498244810744708
2201	21	0.45241516296203343
2201	22	1.3883092601842648
2201	23	1.3534194104506483
2201	24	1.4610651934870873
2201	25	1.7134141899766817
2201	26	1.3506783147408115
2201	27	1.1919103465978438
2201	28	1.8287583076645646
2201	29	1.4450391188672498
2201	30	1.6347455062298764
2201	31	1.6316978160728226
2201	32	1.9441100132506435
2201	33	0.7871072937479369
2201	34	0.4875792610167551
2201	35	1.5025315830003336
2201	36	0.5452158952158896
2201	37	0.6251608200826908
2201	38	1.0604736843018117
2201	39	1.9139539280164186
2201	40	1.8672856223637595
2201	41	1.306151140526154
2201	42	1.5032289758852286
2201	43	1.6011434745809794
2201	44	1.2633736489205205
2201	45	2.1525270302614032
2201	46	-0.37902566183817044
2201	47	0.9292908914002709
2201	48	-0.8477589251026885
2201	49	-4.62009878142689
2201	50	-0.9162614295426721
2201	51	1.1894813519813425
1101	1	1.7792262425074696
1101	2	1.4397007072788384
1101	3	1.8743459231740403
1101	4	-0.12082873723499432
1101	5	1.4828772356115962
1101	6	1.3669047098734595
1101	7	1.0807236600205457
1101	8	-1.6695144699050957
1101	9	0.03950173090797904
1101	10	1.5193691291347662
1101	11	0.9763377247752274
1101	12	1.6801494946026239
1101	13	1.4887125461344297
1101	14	1.9794358852952598
1101	15	1.7128974757880968
1101	16	0.779609886641154
1101	17	1.2669266063797338
1101	18	1.5564091290653792
1101	19	1.2794872662060257
1101	20	1.2751755189255292
1101	21	1.2024151629620334
1101	22	1.1383092601842648
1101	23	1.1034194104506483
1101	24	1.2110651934870873
1101	25	-1.6615858100233183
1101	26	-2.8993216852591885
1101	27	0.9419103465978438
1101	28	1.3287583076645646
1101	29	1.4450391188672498
1101	30	1.3847455062298764
1101	31	0.8816978160728226
1101	32	1.1941100132506435
1101	33	1.5371072937479369
1101	34	0.9875792610167551
1101	35	0.5025315830003336
1101	36	1.2952158952158896
1101	37	1.1251608200826908
1101	38	1.3104736843018117
1101	39	1.4139539280164186
1101	40	1.8672856223637595
1101	41	1.556151140526154
1101	42	0.5032289758852286
1101	43	0.8511434745809794
1101	44	0.7633736489205205
1101	45	1.6525270302614032
1101	46	1.9959743381618296
1101	47	1.6792908914002709
1101	48	2.0272410748973115
1101	49	2.12990121857311
1101	50	2.083738570457328
1101	51	3.0644813519813425
2202	1	0.27922624250746964
2202	2	0.6897007072788384
2202	3	-1.1256540768259597
2202	4	-3.2458287372349943
2202	5	-0.7671227643884038
2202	6	-0.38309529012654053
2202	7	-0.41927633997945435
2202	8	0.9554855300949043
2202	9	-0.960498269092021
2202	10	-1.3556308708652338
2202	11	-0.8986622752247726
2202	12	-3.569850505397376
2202	13	-3.1362874538655703
2202	14	0.9794358852952598
2202	15	-0.2871025242119032
2202	16	-0.47039011335884595
2202	17	0.016926606379733755
2202	18	-3.568590870934621
2202	19	-3.0955127337939743
2202	20	0.02517551892552916
2202	21	-1.1725848370379666
2202	22	-0.7366907398157352
2202	23	1.1034194104506483
2202	24	1.2110651934870873
2202	25	-0.5365858100233183
2202	26	0.6006783147408115
2202	27	-1.6830896534021562
2202	28	1.3287583076645646
2202	29	0.9450391188672498
2202	30	0.13474550622987636
2202	31	0.6316978160728226
2202	32	-2.1808899867493565
2202	33	0.28710729374793686
2202	34	-0.2624207389832449
2202	35	0.002531583000333626
2202	36	-5.45478410478411
2202	37	-0.2498391799173092
2202	38	-1.0645263156981883
2202	39	-1.2110460719835814
2202	40	-2.7577143776362405
2202	41	-2.568848859473846
2202	42	-1.8717710241147714
2202	43	-2.7738565254190206
2202	44	-1.8616263510794795
2202	45	-0.22247296973859676
2202	46	-0.5040256618381704
2202	47	-0.4457091085997291
2202	48	-1.3477589251026885
2202	49	-0.9950987814268899
2202	50	-1.041261429542672
2202	51	-3.6855186480186575
1102	1	-0.020773757492527523
1102	2	-0.9602992927211602
1102	3	-1.8256540768259626
1102	4	0.27917126276500426
1102	5	-1.0171227643884038
1102	6	-1.6830952901265377
1102	7	-0.7192763399794515
1102	8	0.5554855300949058
1102	9	0.38950173090798046
1102	10	-0.1306308708652324
1102	11	1.126337724775226
1102	12	0.3801494946026267
1102	13	0.1387125461344283
1102	14	-0.22056411470474302
1102	15	-1.3371025242119003
1102	16	-1.270390113358843
1102	17	0.41692660637973233
1102	18	-0.743590870934618
1102	19	1.0294872662060257
1102	20	0.3751755189255306
1102	21	-1.7475848370379694
1102	22	-2.5116907398157338
1102	23	-2.0465805895493503
1102	24	0.9110651934870901
1102	25	1.1634141899766846
1102	26	-0.09932168525919138
1102	27	-1.9080896534021576
1102	28	-1.6212416923354382
1102	29	-0.7549608811327531
1102	30	-0.21525449377012507
1102	31	-1.018302183927176
1102	32	-2.6058899867493537
1102	33	-3.5128927062520603
1102	34	-1.9124207389832435
1102	35	-2.097468416999668
1102	36	1.4452158952158882
1102	37	0.7251608200826922
1102	38	0.7104736843018102
1102	39	-0.8360460719835814
1102	40	1.3672856223637595
1102	41	-1.993848859473843
1102	42	-2.2467710241147714
1102	43	-3.248856525419022
1102	44	0.8633736489205219
1102	45	-3.5974729697385968
1102	46	1.095974338161831
1102	47	-1.270709108599732
1102	48	1.7772410748973115
1102	49	1.4799012185731115
1102	50	2.0337385704573308
1102	51	-0.5355186480186589
2203	1	-2.8457737574925304
2203	2	0.6897007072788384
2203	3	-2.6256540768259597
2203	4	-0.7458287372349943
2203	5	-1.3921227643884038
2203	6	-0.7580952901265405
2203	7	-0.5442763399794543
2203	8	-3.6695144699050957
2203	9	-2.085498269092021
2203	10	0.019369129134766183
2203	11	0.7263377247752274
2203	12	0.6801494946026239
2203	13	0.4887125461344297
2203	14	0.22943588529525982
2203	15	-5.287102524211903
2203	16	-2.845390113358846
2203	17	-2.4830733936202662
2203	18	-3.693590870934621
2203	19	-1.7205127337939743
2203	20	0.02517551892552916
2203	21	0.20241516296203343
2203	22	0.6383092601842648
2203	23	0.3534194104506483
2203	24	-3.1639348065129127
2203	25	0.4634141899766817
2203	26	0.35067831474081146
2203	27	-3.308089653402156
2203	28	-1.0462416923354354
2203	29	-2.92996088113275
2203	30	-5.365254493770124
2203	31	-3.6183021839271774
2203	32	-0.3058899867493565
2203	33	0.7871072937479369
2203	34	-2.637420738983245
2203	35	-1.8724684169996664
2203	36	-1.5797841047841104
2203	37	-2.124839179917309
2203	38	-3.3145263156981883
2203	39	-0.7110460719835814
2203	40	-2.0077143776362405
2203	41	0.5561511405261541
2203	42	1.5032289758852286
2203	43	1.3511434745809794
2203	44	-4.9866263510794795
2203	45	-2.4724729697385968
2203	46	0.37097433816182956
2203	47	-4.570709108599729
2203	48	-3.4727589251026885
2203	49	1.37990121857311
2203	50	0.5837385704573279
2203	51	-1.3105186480186575
1103	1	-1.5844101211288972
1103	2	-0.6512083836302551
1103	3	0.23798228681040712
1103	4	0.2882621718559122
1103	5	0.9374226901570495
1103	6	0.525995618964366
1103	7	0.1261782054750924
1103	8	-1.7717871971778223
1103	9	-1.9377709963647476
1103	10	-2.1397217799561403
1103	11	-0.1827531843156791
1103	12	-2.0925777781246495
1103	13	0.28416709158897646
1103	14	0.7067086125679864
1103	15	0.3265338394244637
1103	16	-0.15220829517702583
1103	17	-2.4376188481657195
1103	18	-0.6708635982073474
1103	19	0.9385781752969322
1103	20	1.0479027916528025
1103	21	-0.6839484734015997
1103	22	1.1610365329115382
1103	23	0.39887395590519503
1103	24	0.4383379207598139
1103	25	-0.8547676282051384
1103	26	-1.4447762307137353
1103	27	0.46463761932511716
1103	28	0.6242128531191113
1103	29	0.8541300279581563
1103	30	-1.8425272210428503
1103	31	0.7907887251637291
1103	32	-1.5331627140220832
1103	33	1.3780163846570304
1103	34	0.5330338064713018
1103	35	1.0934406739094271
1103	36	1.6815795315795228
1103	37	0.6706153655372376
1103	38	1.4013827752109052
1103	39	-0.4724097083472145
1103	40	-0.26907801399987363
1103	41	0.3970602314352476
1103	42	0.4350471577034085
1103	43	-1.3761292526917472
1103	44	-1.4638990783522061
1103	45	0.49343612117049673
1103	46	-2.049480207292717
1103	47	-2.934345472236096
1103	48	-0.22275892510268847
1103	49	0.7889921276640166
1103	50	-0.1662614295426721
1103	51	2.473572261072249
2204	1	0.44589290917413393
2204	2	0.7730340406121741
2204	3	1.8743459231740403
2204	4	0.04583792943166998
2204	5	0.14954390227826053
2204	6	0.06134915431790233
2204	7	0.4696125489094314
2204	8	0.3999299745393472
2204	9	-0.8771649357586853
2204	10	-0.008408648643012384
2204	11	-0.6069956085581083
2204	12	0.18014949460262386
2204	13	0.6276014350233154
2204	14	1.3127692186285955
2204	15	0.8517863646769825
2204	16	0.02960988664115405
2204	17	1.128037717490848
2204	18	0.5008535735098221
2204	19	-0.5260682893495314
2204	20	0.44184218559219346
2204	21	1.2857484962953691
2204	22	-0.6672462953712923
2204	23	-0.035469478438237445
2204	24	0.3777318601537516
2204	25	0.18563641219890314
2204	26	0.3229005369630329
2204	27	1.303021457708958
2204	28	1.1898694187756789
2204	29	0.8339280077561355
2204	30	-0.11525449377012364
2204	31	0.7705867049617083
2204	32	1.3052211243617577
2204	33	1.842662849303494
2204	34	1.0986903721278694
2204	35	1.3358649163336693
2204	36	0.4896603396603325
2204	37	0.1251608200826908
2204	38	1.6438070176351474
2204	39	0.38617615023864005
2204	40	0.3672856223637595
2204	41	1.8617066960817112
2204	42	1.5865623092185643
2204	43	1.2400323634698651
2204	44	0.041151426698299076
2204	45	1.5136381413725175
2204	46	1.6070854492729438
2204	47	1.0959575580669352
2204	48	1.8883521860084258
2204	49	2.4354567741286672
2204	50	1.5004052371239922
2204	51	2.0089257964257854
2205	1	0.36255957584080534
2205	2	-0.47696595938782593
2205	3	-0.542320743492624
2205	4	-2.0374954039016586
2205	5	-1.0171227643884038
2205	6	-1.2997619567932048
2205	7	-3.5859430066461186
2205	8	0.45548553009490433
2205	9	0.9561683975746433
2205	10	-0.23063087086523382
2205	11	-0.2736622752247726
2205	12	0.013482827935959563
2205	13	0.405379212801094
2205	14	-0.020564114704740177
2205	15	-0.6204358575452389
2205	16	-0.05372344669218165
2205	17	0.016926606379733755
2205	18	-1.360257537601285
2205	19	1.19615393287269
2205	20	-0.05815781440780654
2205	21	-0.5475848370379666
2205	22	0.13830926018426482
2205	23	-2.063247256216016
2205	24	-2.7889348065129127
2205	25	-1.869919143356654
2205	26	-0.39932168525918854
2205	27	0.6085770132645081
2205	28	-0.6712416923354354
2205	29	0.19503911886724978
2205	30	0.8847455062298764
2205	31	0.2150311494061583
2205	32	-1.0558899867493565
2205	33	-2.046226039585399
2205	34	0.6542459276834194
2205	35	1.0025315830003336
2205	36	1.0452158952158896
2205	37	0.9584941534160265
2205	38	1.3104736843018117
2205	39	0.9972872613497543
2205	40	-0.5493810443029048
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1211	14	-1.6872307813714045
1211	15	0.07400858689921108
1211	16	0.4740543310855969
1211	17	-0.09418450473138051
1211	18	-0.49914642649017793
1211	19	-1.1927349560161957
1211	20	-1.6692689255189137
1211	21	-0.6031403925935237
1211	22	0.5549759268509291
1211	23	-0.14658058954935171
1211	24	0.15550963793153016
1211	25	-2.1476969211344326
1211	26	-0.6770994630369671
1211	27	-4.030311875624378
1211	28	-2.5879083590020997
1211	29	0.05615022997836405
1211	30	0.21807883956321206
1211	31	-2.007191072816063
1211	32	-1.028112208971578
1211	33	0.6204406270812726
1211	34	-1.2346429612054663
1211	35	0.3358649163336693
1211	36	0.7118825618825539
1211	37	-0.09706140213953063
1211	38	0.7549181287462545
1211	39	-4.724934960872467
1211	40	-1.1882699331917976
1211	41	0.750595584970597
1211	42	0.6976734203296715
1211	43	0.7955879190254223
1211	44	-1.4032930177461438
1211	45	0.06919369692806754
1211	46	1.8293076714951653
1211	47	-2.570709108599729
1211	48	-0.33387003621380273
1211	49	1.324345663017553
1211	50	-2.055150318431558
1211	51	-1.8799630924631003
1208	1	0.40422624250746964
1208	2	0.6897007072788384
1208	3	-1.2506540768259597
1208	4	-3.8708287372349943
1208	5	-3.0171227643884038
1208	6	-1.5080952901265405
1208	7	-0.9192763399794543
1208	8	0.45548553009490433
1208	9	0.789501730907979
1208	10	0.019369129134766183
1208	11	0.9763377247752274
1208	12	-0.31985050539737614
1208	13	-0.7612874538655703
1208	14	0.4794358852952598
1208	15	0.2128974757880968
1208	16	-0.22039011335884595
1208	17	-1.8580733936202662
1208	18	-0.8185908709346208
1208	19	-2.0955127337939743
1208	20	-2.099824481074471
1208	21	-1.4225848370379666
1208	22	-5.611690739815735
1208	23	-2.5215805895493517
1208	24	-1.4139348065129127
1208	25	-3.6615858100233183
1208	26	-2.5243216852591885
1208	27	-0.6830896534021562
1208	28	0.5787583076645646
1208	29	0.4450391188672498
1208	30	0.38474550622987636
1208	31	0.3816978160728226
1208	32	1.6941100132506435
1208	33	-9.087892706252063
1208	34	-2.012420738983245
1208	35	-1.9974684169996664
1208	36	0.29521589521588965
1208	37	-2.499839179917309
1208	38	-1.5645263156981883
1208	39	-3.0860460719835814
1208	40	0.3672856223637595
1208	41	1.306151140526154
1208	42	1.2532289758852286
1208	43	-0.1488565254190206
1208	44	1.0133736489205205
1208	45	-1.7224729697385968
1208	46	1.4959743381618296
1208	47	-0.07070910859972912
1208	48	-1.3477589251026885
1208	49	-0.4950987814268899
1208	50	-1.291261429542672
1208	51	1.1894813519813425
1209	1	-1.9207737574925332
1209	2	-3.160299292721163
1209	3	0.0743459231740431
1209	4	0.7791712627650043
1209	5	0.2828772356115934
1209	6	-3.1830952901265377
1209	7	-1.319276339979453
1209	8	-2.5445144699050957
1209	9	0.18950173090797762
1209	10	-4.030630870865231
1209	11	-2.0736622752247698
1209	12	-1.9198505053973776
1209	13	-2.3612874538655717
1209	14	-0.020564114704740177
1209	15	0.5628974757880982
1209	16	1.229609886641157
1209	17	-0.48307339362026624
1209	18	0.6564091290653806
1209	19	-1.8705127337939729
1209	20	-1.524824481074468
1209	21	0.052415162962034856
1209	22	-0.311690739815738
1209	23	0.4534194104506497
1209	24	-1.9889348065129155
1209	25	-1.5365858100233183
1209	26	0.7006783147408129
1209	27	-2.4080896534021576
1209	28	-3.321241692335434
1209	29	-0.8549608811327474
1209	30	-0.9152544937701208
1209	31	-1.018302183927176
1209	32	1.1941100132506435
1209	33	0.4871072937479397
1209	34	-0.11242073898324634
1209	35	0.20253158300033647
1209	36	1.0452158952158896
1209	37	1.1251608200826908
1209	38	1.1104736843018088
1209	39	0.36395392801642146
1209	40	1.3672856223637595
1209	41	1.506151140526157
1209	42	1.4532289758852315
1209	43	1.3511434745809794
1209	44	1.663373648920519
1209	45	1.0025270302614047
1209	46	0.8959743381618281
1209	47	-0.6707091085997305
1209	48	0.5772410748973087
1209	49	-0.42009878142688706
1209	50	0.7337385704573265
1209	51	1.464481351981341
1214	1	1.1125595758408053
1214	2	1.4397007072788384
1214	3	1.207679256507376
1214	4	0.15694904054278425
1214	5	-0.4060116532772895
1214	6	0.28357137654012377
1214	7	0.9140569933538814
1214	8	0.6221521967615686
1214	9	-0.09938715798090669
1214	10	0.6582580180236519
1214	11	1.2818932803307845
1214	12	1.2912606057137381
1214	13	1.0720458794677654
1214	14	-2.5761196702602973
1214	15	1.074008586899211
1214	16	1.3629432199744898
1214	17	0.683593273046398
1214	18	1.1675202401764935
1214	19	1.5850428217615828
1214	20	1.5529532967033077
1214	21	1.2857484962953691
1214	22	1.443864815739822
1214	23	-0.9243583673271303
1214	24	0.599954082375973
1214	25	1.2967475233100174
1214	26	0.9895672036296972
1214	27	0.19191034659784378
1214	28	-0.47679724789099254
1214	29	-2.1660719922438645
1214	30	1.218078839563212
1214	31	1.2150311494061583
1214	32	-1.2503344311937994
1214	33	-1.8240038173631774
1214	34	-0.12353185009435919
1214	35	0.002531583000333626
1214	36	0.7118825618825539
1214	37	0.7918274867493551
1214	38	0.977140350968476
1214	39	-1.2804905164280243
1214	40	-1.7438254887473548
1214	41	0.6394844738594898
1214	42	-1.1912154685592142
1214	43	-0.871078747641242
1214	44	-1.1810707955239224
1214	45	-0.8196951919608182
1214	46	0.6070854492729438
1214	47	0.3181797802891566
1214	48	-0.6672033695471313
1214	49	-1.2312098925380042
1214	50	-0.3884836517648935
1214	51	-0.7688519813519932
1215	1	0.9220833853646155
1215	2	1.4397007072788384
1215	3	0.5886316374597556
1215	4	0.9505998341935751
1215	5	0.6257343784687421
1215	6	0.04547613844489007
1215	7	0.15215223144911505
1215	8	0.6697712443806196
1215	9	1.0752160166222637
1215	10	-0.15920229943666442
1215	11	0.7263377247752274
1215	12	0.18014949460262386
1215	13	-1.1898588824370009
1215	14	-1.163421257561886
1215	15	-1.5371025242119032
1215	16	-1.3989615419302766
1215	17	-1.0545019650488356
1215	18	0.5564091290653792
1215	19	-0.8990841623654049
1215	20	0.13231837606838326
1215	21	0.5952723058191793
1215	22	0.3883092601842648
1215	23	-0.07515201812078232
1215	24	-1.503220520798628
1215	25	-1.6794429528804642
1215	26	-0.25646454240204264
1215	27	0.2633389180264132
1215	28	-0.7069559780497201
1215	29	0.15932483315296508
1215	30	-0.6152544937701236
1215	31	0.3102692446442532
1215	32	1.0512528703934976
1215	33	1.144250150890791
1215	34	-0.012420738983244917
1215	35	-0.426039845571097
1215	36	1.0452158952158896
1215	37	1.1251608200826908
1215	38	1.024759398587527
1215	39	0.4496682137307033
1215	40	1.0815713366494748
1215	41	0.8061511405261541
1215	42	0.9675146901709439
1215	43	-0.5774279539904512
1215	44	0.4062307917776664
1215	45	0.04538417311854914
1215	46	0.49597433816182956
1215	47	0.7150051771145556
1215	48	0.8486696463258809
1215	49	1.4513297900016795
1215	50	0.190881427600182
1215	51	0.20733849483848843
1212	1	0.7792262425074696
1212	2	0.43970070727883837
1212	3	0.37434592317404025
1212	4	-1.20416207056833
1212	5	0.5662105689449319
1212	6	1.2835713765401238
1212	7	0.9140569933538814
1212	8	0.9554855300949043
1212	9	0.9561683975746433
1212	10	0.9360357958014305
1212	11	-1.690328941891437
1212	12	-0.8198505053973761
1212	13	-1.0112874538655703
1212	14	-2.6872307813714045
1212	15	-0.5371025242119032
1212	16	0.19627655330781835
1212	17	-0.14974006028693054
1212	18	-2.360257537601285
1212	19	-1.05384606712731
1212	20	-1.3081578144078065
1212	21	-1.4642515037046309
1212	22	-0.2783574064823995
1212	23	0.8534194104506483
1212	24	0.3777318601537516
1212	25	-0.619919143356654
1212	26	0.9340116480741472
1212	27	-0.3080896534021562
1212	28	0.9120916409979003
1212	29	-0.9716275477994145
1212	30	0.21807883956321206
1212	31	-0.2016355172605131
1212	32	0.3607766799173078
1212	33	0.9537739604146012
1212	34	-0.012420738983244917
1212	35	1.0025315830003336
1212	36	1.3785492285492253
1212	37	-1.958172513250645
1212	38	-0.9395263156981883
1212	39	0.16395392801641862
1212	40	-1.2160477109695762
1212	41	-1.2771821928071816
1212	42	-2.4967710241147714
1212	43	-0.5655231920856849
1212	44	1.0967069822538562
1212	45	0.5691936969280675
1212	46	0.32930767149516527
1212	47	0.2626242247336066
1212	48	0.6105744082306472
1212	49	-1.7867654480935542
1212	50	-1.3329280962093364
1212	51	-2.268851981351993
1213	1	-2.4025919393107102
1213	2	-4.196662929084795
1213	3	-0.6711086222805065
1213	4	-0.7117378281440878
1213	5	-2.971668218933857
1213	6	-1.1103680173992672
1213	7	-0.23745815816127447
1213	8	0.3191218937312712
1213	9	-0.39231645091020084
1213	10	-1.048812689047054
1213	11	-1.6372986388611395
1213	12	-0.2743959599428294
1213	13	-0.0794692720473904
1213	14	0.3430722489316267
1213	15	0.5083520212426436
1213	16	0.21142806845933393
1213	17	0.19874478819791364
1213	18	0.9655000381562857
1213	19	0.2113054480242056
1213	20	-0.5884608447108377
1213	21	-0.9566757461288731
1213	22	0.2519456238206317
1213	23	0.21705577408701515
1213	24	0.4383379207598139
1213	25	0.8725050990675882
1213	26	-1.6265944125319152
1213	27	-1.989907835220336
1213	28	-1.2848780559718023
1213	29	-1.23677906295093
1213	30	-2.4788908574064905
1213	31	-1.4819385475635443
1213	32	-0.9877081685675364
1213	33	-1.2583472517066099
1213	34	0.26030653374402846
1213	35	-1.724741144272393
1213	36	-1.1366022866022902
1213	37	-1.9657482708264027
1213	38	-4.5077081338800085
1213	39	0.3457721098345985
1213	40	-1.9054416503635139
1213	41	-0.6938488594738459
1213	42	-3.474043751387498
1213	43	-3.921583798146294
1213	44	-3.1002627147158464
1213	45	1.1297997575341299
1213	46	-1.3222074800199906
1213	47	-0.20707274496336225
1213	48	-2.4045771069208683
1213	49	-1.5746442359723432
1213	50	-0.1662614295426721
1213	51	-0.4355186480186575
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	1.0
19	0.0
20	0.0
21	2.0
22	3.0
23	12.0
24	8.0
25	19.0
26	30.0
27	46.0
28	63.0
29	90.0
30	96.0
31	132.0
32	152.0
33	172.0
34	265.0
35	314.0
36	381.0
37	570.0
38	1049.0
39	3127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.22615219721329	17.133670188332566	12.5708161077936	37.06936150666054
2	12.490433185366602	18.888718812184297	48.15551813868055	20.46532986376856
3	16.546762589928058	22.868513699678555	38.58870350528088	21.996020205112508
4	11.572018980560232	30.001530690341344	31.226082963416502	27.20036736568192
5	11.61793969080055	33.17005969692331	42.139905097198834	13.0720955150773
6	26.94015000765345	45.645185978876476	14.204806367671821	13.209857645798257
7	21.950099494872187	29.89438236644727	17.893770090310728	30.261748048369814
8	19.50099494872187	44.680851063829785	17.93969080055105	17.87846318689729
9	20.358181539874483	17.128424919638757	18.87341190877086	43.639981631715905
10	14.801775600795958	39.26220725547222	27.123832848614725	18.8121842951171
11	26.40440838818307	21.58273381294964	34.777284555334454	17.235573243532833
12	19.164243073626206	23.25118628501454	25.057400887800398	32.52716975355885
13	21.475585489055565	19.837746823817543	37.3947650390326	21.29190264809429
14	17.740701056176338	20.75616102862391	22.807286086024796	38.69585182917496
15	19.087708556559008	26.603398132557782	21.460278585642126	32.848614725241084
16	32.11879975505205	26.561543172075936	22.933251684017147	18.386405388854868
17	18.76626358487678	39.15505893157814	23.86346242155212	18.21521506199296
18	31.501607224858414	24.062452165926835	24.67472830246441	19.761212306750345
19	17.34272156742691	24.67472830246441	25.730904637991735	32.25164549211694
20	18.18460125516608	22.547068727996326	25.53191489361702	33.73641512322057
21	17.54171131180162	25.10332159804072	25.133935404867596	32.221031685290065
22	18.475432420021427	37.34884432879228	25.822746058472372	18.352977192713915
23	19.01117403949181	23.786927904484923	24.919638757079444	32.28225929894382
24	32.17511097504975	25.80743915505893	24.13898668299403	17.87846318689729
25	19.88366753405786	37.88458594826267	25.118628501454154	17.113118016225318
26	31.83835909995408	25.86866676871269	25.057400887800398	17.235573243532833
27	18.016225317618247	38.77238634624216	24.996173274146642	18.21521506199296
28	18.276442675646717	24.751262819531608	25.088014694627276	31.8842798101944
29	18.567273840502065	38.175417113118016	25.24108372876167	18.016225317618247
30	18.24582886881984	37.9764273687433	25.149242308281032	18.628501454155824
31	18.18460125516608	25.195163018521356	24.858411143425684	31.761824582886884
32	17.92438389713761	36.55288535129343	27.246288075922244	18.276442675646717
33	17.66416653910914	25.455380376549826	38.69585182917496	18.18460125516608
34	18.44481861319455	37.318230521965404	25.86866676871269	18.368284096127354
35	17.847849380070414	36.767181999081586	26.205418643808358	19.179549977039645
36	17.694780345936017	24.843104240012245	39.52242461350069	17.93969080055105
37	17.801928669830094	35.92530231134241	27.108525945201283	19.164243073626206
38	17.954997703964487	21.705189040257157	40.31838359099954	20.021429664778815
39	17.434562987907544	21.705189040257157	27.185060462268485	33.67518750956682
40	17.480483698147864	22.10316852900658	40.593907852441454	19.822439920404104
41	19.225470687279962	21.628654523189958	25.853359865299254	33.29251492423083
42	30.567886116638604	22.883820603091994	27.552426144191028	18.99586713607837
43	16.317159038726466	23.970610745446198	39.476503903260365	20.235726312566968
44	17.449869891320986	36.09367824889025	26.909536200826572	19.54691565896219
45	17.771314863003216	22.7307515689576	41.02250114801775	18.475432420021427
46	31.21077606000306	22.210316852900657	25.899280575539567	20.679626511556712
47	16.45492116944742	22.638910148476963	25.960508189193327	34.94566049288229
48	17.725394152762895	22.48584111434257	24.858411143425684	34.93035358946885
49	30.368896372263894	22.7307515689576	26.389101484769633	20.511250574008876
50	16.684524720649012	22.13378233583346	39.50711771008725	21.674575233430275
51	16.301852135313027	22.087861625593142	26.787080973519057	34.82320526557478
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	39.5
2	4.0
3	6.5
4	9.0
5	12.5
6	16.0
7	11.0
8	6.0
9	9.0
10	12.0
11	20.5
12	29.0
13	36.0
14	43.0
15	45.0
16	47.0
17	45.0
18	43.0
19	41.5
20	40.0
21	41.0
22	42.0
23	38.5
24	35.0
25	45.0
26	51.5
27	48.0
28	71.5
29	95.0
30	116.5
31	138.0
32	180.0
33	222.0
34	241.5
35	261.0
36	297.5
37	334.0
38	333.5
39	333.0
40	367.0
41	401.0
42	396.0
43	391.0
44	424.0
45	457.0
46	828.5
47	1200.0
48	794.0
49	388.0
50	380.0
51	372.0
52	363.5
53	355.0
54	310.0
55	265.0
56	242.0
57	219.0
58	193.5
59	168.0
60	143.5
61	119.0
62	117.0
63	115.0
64	97.5
65	80.0
66	64.5
67	49.0
68	36.5
69	24.0
70	19.0
71	14.0
72	14.0
73	14.0
74	10.5
75	5.5
76	4.0
77	3.0
78	2.0
79	1.0
80	0.0
81	1.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030613806826878923
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.015306903413439462
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6533.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.52456757997857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.19752159218926	80.05510485228838
2	1.2579797221179123	2.0511250574008875
3	0.13143071723619978	0.3214449716822287
4	0.0938790837401427	0.3061380682687892
5	0.01877581674802854	0.0765345170671973
6	0.07510326699211416	0.3673656819225471
7	0.056327450244085614	0.3214449716822287
8	0.01877581674802854	0.1224552273075157
9	0.0	0.0
>10	0.0938790837401427	1.193938466248278
>50	0.03755163349605708	2.2654217051890404
>100	0.0	0.0
>500	0.01877581674802854	12.919026480942906
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	844	12.919026480942906	No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT	79	1.2092453696617176	RNA PCR Primer, Index 44 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	69	1.0561763355273228	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26	0.39797948874942596	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.26021735802847085	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.22960355120159193	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	10	0.1530690341343946	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT	10	0.1530690341343946	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.1224552273075157	No Hit
TATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTT	7	0.10714832389407623	RNA PCR Primer, Index 44 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	7	0.10714832389407623	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.10714832389407623	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10	0.0	0.0	0.0
11	0.0	0.0	0.0
12	0.0	0.0	0.0
13	0.0	0.0	0.0
14	0.0	0.0	0.0
15	0.0	0.0	0.0
16	0.0	0.0	0.0
17	0.0	0.0	0.0
18	0.0	0.0	0.0
19	0.0	0.0	0.0
20	0.0	0.0	0.015306903413439462
21	0.0	0.0	0.015306903413439462
22	0.0	0.0	0.04592071024031839
23	0.0	0.0	0.04592071024031839
24	0.0	0.0	0.04592071024031839
25	0.0	0.0	0.04592071024031839
26	0.0	0.0	0.06122761365375785
27	0.0	0.0	0.06122761365375785
28	0.0	0.0	0.06122761365375785
29	0.0	0.0	0.13776213072095514
30	0.0	0.0	0.21429664778815247
31	0.0	0.0	0.5051278126435023
32	0.0	0.0	0.8724934945660493
33	0.0	0.0	1.331700596969233
34	0.0	0.0	2.0052043471605696
35	0.0	0.0	2.6940150007653454
36	0.0	0.0	3.321598040716363
37	0.0	0.0	4.301239859176489
38	0.0	0.0	4.990050512781265
39	0.0	0.0	5.816623297106995
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCCAC	85	0.0	39.705883	17
GTATGCC	85	0.0	39.705883	42
ATCTCGT	85	0.0	39.705883	37
ATCTCCG	85	0.0	39.705883	10
GCCCACG	85	0.0	39.705883	18
TGCCGTC	85	0.0	39.705883	45
CCACGAG	85	0.0	39.705883	20
TATGCCG	85	0.0	39.705883	43
CATCTCC	85	0.0	39.705883	9
GAGCCCA	85	0.0	39.705883	16
CACGAGA	85	0.0	39.705883	21
CCCACGA	85	0.0	39.705883	19
TCTCGTA	85	0.0	39.705883	38
ACGAGAC	85	0.0	39.705883	22
ATGCCGT	85	0.0	39.705883	44
CGTATGC	85	0.0	39.705883	41
CCGAGCC	85	0.0	39.705883	14
TCGTATG	85	0.0	39.705883	40
TCTCCGA	85	0.0	39.705883	11
TCCGAGC	85	0.0	39.705883	13
>>END_MODULE