FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_83.34100000006d44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_83.34100000006d44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6533
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC84412.919026480942906No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT791.2092453696617176RNA PCR Primer, Index 44 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA691.0561763355273228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.39797948874942596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.26021735802847085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.22960355120159193No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC100.1530690341343946No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT100.1530690341343946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.1224552273075157No Hit
TATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTT70.10714832389407623RNA PCR Primer, Index 44 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC70.10714832389407623No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10714832389407623No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC850.039.70588317
GTATGCC850.039.70588342
ATCTCGT850.039.70588337
ATCTCCG850.039.70588310
GCCCACG850.039.70588318
TGCCGTC850.039.70588345
CCACGAG850.039.70588320
TATGCCG850.039.70588343
CATCTCC850.039.7058839
GAGCCCA850.039.70588316
CACGAGA850.039.70588321
CCCACGA850.039.70588319
TCTCGTA850.039.70588338
ACGAGAC850.039.70588322
ATGCCGT850.039.70588344
CGTATGC850.039.70588341
CCGAGCC850.039.70588314
TCGTATG850.039.70588340
TCTCCGA850.039.70588311
TCCGAGC850.039.70588313
Produced by FastQC (version 0.11.2)