##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_82.34100000006d37.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30034 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.25531064793234 38.0 38.0 38.0 32.0 38.0 2 36.263368182726246 38.0 38.0 38.0 32.0 38.0 3 36.24029433308917 38.0 38.0 38.0 32.0 38.0 4 36.3726110408204 38.0 38.0 38.0 32.0 38.0 5 36.40127855097556 38.0 38.0 38.0 32.0 38.0 6 38.270526736365454 40.0 38.0 40.0 38.0 40.0 7 38.21052806818938 40.0 38.0 40.0 38.0 40.0 8 38.16934141306519 40.0 38.0 40.0 38.0 40.0 9 38.216221615502434 40.0 38.0 40.0 38.0 40.0 10 38.14813211693414 40.0 38.0 40.0 38.0 40.0 11 38.15395884664047 40.0 38.0 40.0 38.0 40.0 12 38.084970366917496 40.0 38.0 40.0 38.0 40.0 13 38.09099687021376 40.0 38.0 40.0 38.0 40.0 14 38.047712592395285 40.0 38.0 40.0 38.0 40.0 15 38.03339548511687 40.0 38.0 40.0 32.0 40.0 16 38.03502696943464 40.0 38.0 40.0 32.0 40.0 17 38.042585070253715 40.0 38.0 40.0 32.0 40.0 18 38.00825730838383 40.0 38.0 40.0 32.0 40.0 19 38.06209629087035 40.0 38.0 40.0 38.0 40.0 20 38.0419524538856 40.0 38.0 40.0 38.0 40.0 21 38.07125258040887 40.0 38.0 40.0 38.0 40.0 22 38.0966904175268 40.0 38.0 40.0 38.0 40.0 23 38.05903309582473 40.0 38.0 40.0 32.0 40.0 24 38.02367317040687 40.0 38.0 40.0 32.0 40.0 25 38.05257374975028 40.0 38.0 40.0 32.0 40.0 26 37.96986748351868 40.0 38.0 40.0 32.0 40.0 27 37.96577212492509 40.0 38.0 40.0 32.0 40.0 28 37.927915029633084 40.0 38.0 40.0 32.0 40.0 29 37.90930279017114 40.0 38.0 40.0 32.0 40.0 30 37.91173336884864 40.0 38.0 40.0 32.0 40.0 31 37.881234600785774 40.0 38.0 40.0 32.0 40.0 32 37.85416527935007 40.0 38.0 40.0 32.0 40.0 33 37.80755144169941 40.0 38.0 40.0 32.0 40.0 34 37.82333355530399 40.0 38.0 40.0 32.0 40.0 35 37.834254511553574 40.0 38.0 40.0 32.0 40.0 36 37.74848505027635 40.0 38.0 40.0 32.0 40.0 37 37.78221349137644 40.0 38.0 40.0 32.0 40.0 38 37.77958313910901 40.0 38.0 40.0 32.0 40.0 39 37.70240394219884 40.0 38.0 40.0 32.0 40.0 40 37.66138376506626 40.0 38.0 40.0 32.0 40.0 41 37.69554504894453 40.0 38.0 40.0 32.0 40.0 42 37.65449157621362 40.0 38.0 40.0 32.0 40.0 43 37.62196177665312 40.0 38.0 40.0 32.0 40.0 44 37.53159752280749 40.0 38.0 40.0 32.0 40.0 45 37.43760404874475 40.0 38.0 40.0 32.0 40.0 46 37.312545781447696 40.0 38.0 40.0 32.0 40.0 47 37.273090497436236 40.0 38.0 40.0 32.0 40.0 48 37.227808483718455 40.0 38.0 40.0 32.0 40.0 49 37.20503429446627 40.0 38.0 40.0 32.0 40.0 50 37.15941932476527 40.0 38.0 40.0 32.0 40.0 51 36.67460211760005 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.3141782048782673 2201 2 0.4810821903964069 2201 3 -0.11508569591826046 2201 4 0.42341754518937336 2201 5 0.19298295831368506 2201 6 -0.046116180177861565 2201 7 0.16005888630902376 2201 8 0.9295618699573538 2201 9 0.8015465326612556 2201 10 -0.5512290385174836 2201 11 -0.40558039674192514 2201 12 0.01802450132158384 2201 13 0.5927255802486613 2201 14 0.06792801850138375 2201 15 0.35743698748455444 2201 16 0.8010768441718596 2201 17 0.11685132897518713 2201 18 0.7782969857904334 2201 19 0.7981897511725862 2201 20 0.5927139626741393 2201 21 -0.3172423267937958 2201 22 -0.4076561794375877 2201 23 0.08908899151418126 2201 24 0.7351625290459509 2201 25 0.9625608279860032 2201 26 0.9266019203098423 2201 27 0.5551232824032439 2201 28 0.7110804121953294 2201 29 0.16915357925707042 2201 30 -0.22518346220552843 2201 31 0.2715623105547351 2201 32 -0.35067756968956587 2201 33 0.42144639199270273 2201 34 0.798180488922533 2201 35 -0.02097247919947165 2201 36 1.1380194760179307 2201 37 0.7250248265459618 2201 38 0.76669301542033 2201 39 1.0069638496519744 2201 40 0.641098402157219 2201 41 1.2546357745770962 2201 42 0.9203633945610861 2201 43 1.091310926549177 2201 44 0.7905912916345486 2201 45 0.8319237393990875 2201 46 0.5726047605668256 2201 47 -0.1485248602245477 2201 48 0.25831508745212517 2201 49 0.20286601464093224 2201 50 0.2417188629120588 2201 51 0.22830726284029623 1101 1 0.29035517595030313 1101 2 0.4260623378723025 1101 3 0.5559296188860543 1101 4 0.8964748339017987 1101 5 0.5333119429988855 1101 6 0.9657953342861205 1101 7 0.8395824257304625 1101 8 0.670344626622132 1101 9 0.8026809626102036 1101 10 1.2366325610287419 1101 11 0.4174485312217726 1101 12 0.2171169573624212 1101 13 0.977297332942932 1101 14 -0.28885020044359777 1101 15 0.596234491738663 1101 16 0.4601806445121923 1101 17 -0.16562172831352484 1101 18 1.0397830890235582 1101 19 0.898019586680249 1101 20 1.1899913307966585 1101 21 1.0174145081466435 1101 22 1.0234272011636563 1101 23 1.1452432739872407 1101 24 1.2303412017629114 1101 25 1.211568201780672 1101 26 0.46602336103587305 1101 27 1.1546695104236662 1101 28 1.1200424087920382 1101 29 1.25650468532627 1101 30 1.1128766625817619 1101 31 1.268159020707884 1101 32 1.209163610117585 1101 33 1.031202489553678 1101 34 1.372202043091562 1101 35 1.4475470897171476 1101 36 1.327469277492689 1101 37 1.4442534141806718 1101 38 1.3883606274452873 1101 39 -0.14107925868608362 1101 40 0.9076894401606239 1101 41 0.7906539254562759 1101 42 0.9209306095355672 1101 43 0.8025985045412298 1101 44 1.5444199927122568 1101 45 0.008894811435389727 1101 46 1.1596722591487918 1101 47 1.1804598249711376 1101 48 1.7727790693012508 1101 49 1.0417769618899442 1101 50 0.8945832985331634 1101 51 1.5039737404353062 2202 1 0.9647737806014618 2202 2 0.7283879192676537 2202 3 0.5791854328395445 2202 4 0.4778701827390037 2202 5 0.9519165941616734 2202 6 0.33788835754193514 2202 7 0.5140010303816283 2202 8 1.0191818359244564 2202 9 0.8724484044706671 2202 10 0.07384186335432474 2202 11 0.09186713587293838 2202 12 0.4264192829438187 2202 13 0.8377624492219979 2202 14 0.7111497995564022 2202 15 0.3404205382502923 2202 16 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2 -0.03905394119746575 1102 3 0.18383659563023969 1102 4 0.3383352990180768 1102 5 0.6728468267198124 1102 6 0.5704464970768228 1102 7 0.4209777745676746 1102 8 0.8331353242965491 1102 9 0.570122823075323 1102 10 -0.15871627618055584 1102 11 -0.6058072827317176 1102 12 0.4264192829438187 1102 13 -0.8366561554291607 1102 14 0.1530102646726803 1102 15 -1.0316724850055223 1102 16 0.18111087707033136 1102 17 0.1599596670353165 1102 18 0.5514109960003069 1102 19 0.014298656447685687 1102 20 -0.1588458785056659 1102 21 -0.5872366546440517 1102 22 0.06993882907063664 1102 23 -0.017547423687176433 1102 24 0.5791784110652358 1102 25 0.8394751785248573 1102 26 0.21020940754750228 1102 27 0.9686229987957589 1102 28 0.7944610134431969 1102 29 1.2099930574192896 1102 30 0.9268301509538546 1102 31 0.15187995094044737 1102 32 -0.20944104104520989 1102 33 -0.6199603011439976 1102 34 0.5349927407659862 1102 35 0.37777964785667706 1102 36 -0.20741444343754267 1102 37 0.44425341418067177 1102 38 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-0.6898002118826838 1213 49 -1.7878213467781308 1213 50 -1.0944230439615552 1213 51 -2.244863468867017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 2.0 20 1.0 21 0.0 22 6.0 23 21.0 24 46.0 25 75.0 26 132.0 27 189.0 28 286.0 29 327.0 30 437.0 31 542.0 32 695.0 33 805.0 34 1011.0 35 1284.0 36 1766.0 37 2574.0 38 4689.0 39 15145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.712886786796794 18.732305232654962 13.119941378276653 28.43486660227159 2 15.588999134314443 20.94626090430845 38.47972298062196 24.985016980755145 3 19.727642005726842 22.617699940067922 29.516547912365986 28.138110141839253 4 14.177265765465805 18.575614303789038 34.5341945794766 32.71292535126856 5 15.355929946061131 35.386561896517286 33.908237331024836 15.349270826396749 6 32.79949390690551 37.074648731437705 15.805420523406804 14.320436838249984 7 28.284610774455615 31.540920290337617 19.617766531264564 20.556702403942197 8 25.28134780582007 35.7195178797363 19.827528800692548 19.171605513751082 9 24.74528867283745 18.089498568289272 20.43017913031897 36.735033628554305 10 16.59452620363588 29.802890057934338 30.601984417659985 23.000599320769794 11 32.94266497968968 23.966171672104945 24.066058467070654 19.025104881134713 12 23.43677165878671 25.248052207498166 28.00492774855164 23.310248385163483 13 27.049344076713062 22.37131251248585 26.9328094825864 23.64653392821469 14 21.255909968702138 22.094959046414065 26.573217020709862 30.075913964173935 15 23.207032030365585 29.15695545048945 23.539988013584605 24.096024505560365 16 23.936205633615238 28.81401078777386 25.371245921289205 21.878537657321704 17 21.785309982020376 29.8827994939069 26.73636545248718 21.595525071585538 18 23.23033894919092 27.382300059932074 27.994939069055068 21.392421921821935 19 21.941799294133315 27.372311380435505 28.001598188719452 22.684291136711725 20 22.091629486581873 26.73636545248718 27.911700073250316 23.260304987680627 21 21.389092361989746 26.782979290137842 28.17806485982553 23.64986348804688 22 21.685423187054674 28.377838449756943 28.341213291602852 21.595525071585538 23 21.835253379503232 26.772990610641273 28.13145102217487 23.260304987680627 24 23.563294932409935 26.69974029433309 28.30791769328095 21.429047079976026 25 22.05500432842778 28.194712658986482 27.988279949390694 21.762003063195046 26 23.41013518012919 26.73303589265499 28.620896317506826 21.235932609708996 27 21.79529866151695 28.69081707398282 27.855097556103082 21.658786708397148 28 21.439035759472596 27.26576546580542 27.342345341945794 23.952853432776188 29 21.771991742691615 29.22687620696544 27.698608243990147 21.3025238063528 30 21.60218419124992 28.477725244722645 28.078178064859827 21.84191249916761 31 22.158220683225675 26.49996670440168 27.77851767996271 23.563294932409935 32 21.85190117866418 28.101484983685154 27.911700073250316 22.134913764400345 33 21.52227475527735 27.07265099553839 29.7829126989412 21.622161550243057 34 21.682093627222482 28.054871146034493 28.451088766065126 21.8119464606779 35 22.151561563561298 28.67416927482187 27.685290004661383 21.488979156955452 36 21.858560298328563 26.273556635812746 30.019311447026702 21.84857161883199 37 22.118265965239395 27.675301325164813 27.615369248185388 22.591063461410403 38 21.29919424652061 25.51108743424119 30.478790703868945 22.710927615369247 39 21.6421389092362 25.760804421655457 28.44442964640075 24.1526270227076 40 21.768662182859426 25.43783711793301 30.748485050276354 22.04501564893121 41 22.264766597855765 25.12485849370713 28.650862355996537 23.959512552440568 42 23.44676033828328 25.910634614104016 28.49770260371579 22.144902443896918 43 21.459013118465737 25.50109875474462 30.202437237797163 22.837450888992475 44 21.245921289205565 27.299061064127322 29.047079976027167 22.407937670639942 45 21.582206832256777 25.75081574215889 30.035959246187655 22.631018179396683 46 22.817473529999337 25.7807817806486 28.277951654791238 23.12379303456083 47 20.869681028168078 26.090430845042285 28.431111407071985 24.608776719717653 48 20.809748951188652 26.023839648398482 28.17140574016115 24.995005660251714 49 22.77751881201305 25.910634614104016 27.92834787241127 23.383498701471666 50 20.836385429846175 26.420057268429115 29.40667243790371 23.336884863821002 51 20.400892351746414 26.001398461692137 28.149036060333632 25.448673126227817 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 136.0 1 75.5 2 15.0 3 18.5 4 22.0 5 23.5 6 25.0 7 25.5 8 26.0 9 32.0 10 38.0 11 55.0 12 72.0 13 112.5 14 153.0 15 187.0 16 221.0 17 221.0 18 221.0 19 211.5 20 202.0 21 187.5 22 173.0 23 171.0 24 169.0 25 197.5 26 267.5 27 309.0 28 364.0 29 419.0 30 535.5 31 652.0 32 790.5 33 929.0 34 1062.0 35 1195.0 36 1344.5 37 1494.0 38 1607.5 39 1721.0 40 1774.0 41 1827.0 42 1845.0 43 1863.0 44 1984.5 45 2106.0 46 2396.5 47 2687.0 48 2411.0 49 2135.0 50 2105.0 51 2075.0 52 2031.0 53 1987.0 54 1849.0 55 1711.0 56 1574.5 57 1438.0 58 1260.0 59 1082.0 60 994.0 61 906.0 62 772.5 63 639.0 64 540.0 65 441.0 66 372.0 67 303.0 68 233.5 69 164.0 70 139.0 71 114.0 72 91.0 73 68.0 74 48.0 75 26.5 76 25.0 77 18.0 78 11.0 79 7.0 80 3.0 81 2.0 82 1.0 83 1.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03662515815409203 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.003329559832190184 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 30034.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.66917811848042 #Duplication Level Percentage of deduplicated Percentage of total 1 96.42674016965881 89.35786760166545 2 3.0385520966280697 5.631602509294174 3 0.28741516995138167 0.7990358273453372 4 0.10342305191419507 0.3833651687757356 5 0.035711829122902085 0.1654692926963475 6 0.02509112316786134 0.13951042572211636 7 0.02799750865482147 0.18161542814851436 8 0.007755703718248676 0.0574971751440438 9 0.003794564297802559 0.03164752393155827 >10 0.02194734976895945 0.28053202086834267 >50 0.01078571663802529 0.5597219576710689 >100 0.01078571647892565 2.412135068737323 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 487 1.62149563827662 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 128 0.42618365852034357 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 109 0.3629220217087301 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60 0.19977358993141106 No Hit TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT 54 0.17979623093826996 RNA PCR Primer, Index 44 (95% over 22bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 54 0.17979623093826996 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0033295598321901845 18 0.0 0.0 0.0033295598321901845 19 0.0 0.0 0.0033295598321901845 20 0.0 0.0 0.0033295598321901845 21 0.0 0.0 0.0033295598321901845 22 0.0 0.0 0.0033295598321901845 23 0.0 0.0 0.0033295598321901845 24 0.0 0.0 0.006659119664380369 25 0.0 0.0 0.013318239328760738 26 0.0 0.0 0.019977358993141108 27 0.0 0.0 0.026636478657521476 28 0.0 0.0 0.0432842778184724 29 0.0 0.0 0.06992075647599387 30 0.0 0.0 0.12319371379103683 31 0.0 0.0 0.3362855430512086 32 0.0 0.0 0.5460478124791902 33 0.0 0.0 0.8490377572084971 34 0.0 0.0 1.1886528600918957 35 0.0 0.0 1.5748818006259573 36 0.0 0.0 1.98774721981754 37 0.0 0.0 2.5204767929679694 38 0.0 0.0 3.07651328494373 39 0.0 0.0 3.6392088965838716 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGCC 80 0.0 42.18594 42 ATCTCGT 80 0.0 42.18594 37 GCCCACG 80 0.0 42.18594 18 TGCCGTC 80 0.0 42.18594 45 TATGCCG 80 0.0 42.18594 43 CAACATA 80 0.0 42.18594 28 CACGAGA 80 0.0 42.18594 21 ACGAGAC 80 0.0 42.18594 22 TATCTCG 80 0.0 42.18594 36 ACATAAT 80 0.0 42.18594 30 ATGCCGT 80 0.0 42.18594 44 CGAGCCC 80 0.0 42.18594 15 CCGAGCC 80 0.0 42.18594 14 CGAGACA 80 0.0 42.18594 23 TCCGAGC 80 0.0 42.18594 13 ATATCTC 80 0.0 42.18594 35 CTCGTAT 80 0.0 42.18594 39 CTCCGAG 80 0.0 42.18594 12 ATCTCCG 85 0.0 39.704414 10 CCACGAG 85 0.0 39.704414 20 >>END_MODULE