FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_82.34100000006d37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_82.34100000006d37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30034
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC4871.62149563827662No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.42618365852034357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.3629220217087301No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.19977358993141106No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT540.17979623093826996RNA PCR Primer, Index 44 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.17979623093826996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC800.042.1859442
ATCTCGT800.042.1859437
GCCCACG800.042.1859418
TGCCGTC800.042.1859445
TATGCCG800.042.1859443
CAACATA800.042.1859428
CACGAGA800.042.1859421
ACGAGAC800.042.1859422
TATCTCG800.042.1859436
ACATAAT800.042.1859430
ATGCCGT800.042.1859444
CGAGCCC800.042.1859415
CCGAGCC800.042.1859414
CGAGACA800.042.1859423
TCCGAGC800.042.1859413
ATATCTC800.042.1859435
CTCGTAT800.042.1859439
CTCCGAG800.042.1859412
ATCTCCG850.039.70441410
CCACGAG850.039.70441420