FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_81.34100000006d2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_81.34100000006d2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27063
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4781.7662491224180614No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC2100.7759671876732069No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.7464065329047038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.4434098215275468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.34733769352991173No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.2327901563019621No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.10346229168976093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC302.087676E-645.00000445
ATAATAT253.7903912E-545.032
ACATAAT253.7903912E-545.030
TAATATC253.7903912E-545.033
ATATCTC253.7903912E-545.035
GAGACAA253.7903912E-545.024
CATAATA253.7903912E-545.031
GCCCACG356.0251805E-638.57142618
CCACGAG356.0251805E-638.57142620
CCCACGA356.0251805E-638.57142619
ACGAGAC356.0251805E-638.57142622
TATCTCG356.0251805E-638.57142636
ATGCCGT356.0251805E-638.57142644
CGAGCCC356.0251805E-638.57142615
CTCCGAG356.0251805E-638.57142612
CAACATA301.11072644E-437.50000428
AATATCT301.11072644E-437.50000434
AGACAAC301.11072644E-437.50000425
AACATAA301.11072644E-437.50000429
GTATCAA552.5429472E-936.818181