##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_80.34100000006d1d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27082 Sequences flagged as poor quality 0 Sequence length 51 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.398862713241265 38.0 38.0 38.0 32.0 38.0 2 36.333099475666494 38.0 38.0 38.0 32.0 38.0 3 36.36131009526623 38.0 38.0 38.0 32.0 38.0 4 36.40680156561554 38.0 38.0 38.0 32.0 38.0 5 36.434827560741454 38.0 38.0 38.0 32.0 38.0 6 38.324791374344585 40.0 38.0 40.0 38.0 40.0 7 38.24857839155158 40.0 38.0 40.0 38.0 40.0 8 38.225168008271176 40.0 38.0 40.0 38.0 40.0 9 38.23934716786057 40.0 38.0 40.0 38.0 40.0 10 38.14090539841961 40.0 38.0 40.0 38.0 40.0 11 38.10375895428698 40.0 38.0 40.0 32.0 40.0 12 38.031792334391845 40.0 38.0 40.0 32.0 40.0 13 38.02056716638358 40.0 38.0 40.0 32.0 40.0 14 37.97825123698397 40.0 38.0 40.0 32.0 40.0 15 37.97234325382173 40.0 38.0 40.0 32.0 40.0 16 38.00845580090097 40.0 38.0 40.0 32.0 40.0 17 38.005501809319846 40.0 38.0 40.0 32.0 40.0 18 38.02470275459715 40.0 38.0 40.0 32.0 40.0 19 38.07207739457942 40.0 38.0 40.0 38.0 40.0 20 38.103906653866034 40.0 38.0 40.0 38.0 40.0 21 38.12096595524703 40.0 38.0 40.0 38.0 40.0 22 38.11793811387638 40.0 38.0 40.0 38.0 40.0 23 38.112879403293704 40.0 38.0 40.0 38.0 40.0 24 38.06188612362455 40.0 38.0 40.0 32.0 40.0 25 38.060963001255445 40.0 38.0 40.0 32.0 40.0 26 38.010375895428695 40.0 38.0 40.0 32.0 40.0 27 37.99970460084189 40.0 38.0 40.0 32.0 40.0 28 37.93379366368806 40.0 38.0 40.0 32.0 40.0 29 37.946865076434534 40.0 38.0 40.0 32.0 40.0 30 37.94719740048741 40.0 38.0 40.0 32.0 40.0 31 37.90477069640352 40.0 38.0 40.0 32.0 40.0 32 37.88261575954508 40.0 38.0 40.0 32.0 40.0 33 37.87958791817443 40.0 38.0 40.0 32.0 40.0 34 37.8894837899712 40.0 38.0 40.0 32.0 40.0 35 37.84491544199099 40.0 38.0 40.0 32.0 40.0 36 37.79787312606159 40.0 38.0 40.0 32.0 40.0 37 37.78661103315856 40.0 38.0 40.0 32.0 40.0 38 37.74185806070453 40.0 38.0 40.0 32.0 40.0 39 37.647994978214314 40.0 38.0 40.0 32.0 40.0 40 37.63857913004948 40.0 38.0 40.0 32.0 40.0 41 37.669411417177464 40.0 38.0 40.0 32.0 40.0 42 37.59858946902001 40.0 38.0 40.0 32.0 40.0 43 37.58946902001329 40.0 38.0 40.0 32.0 40.0 44 37.45742559633705 40.0 38.0 40.0 32.0 40.0 45 37.45406543091352 40.0 38.0 40.0 32.0 40.0 46 37.30271028727568 40.0 38.0 40.0 32.0 40.0 47 37.264271471826305 40.0 38.0 40.0 32.0 40.0 48 37.151133594269254 40.0 38.0 40.0 32.0 40.0 49 37.06155379957167 40.0 38.0 40.0 32.0 40.0 50 36.97001698545159 40.0 38.0 40.0 32.0 40.0 51 36.73535927922605 40.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.9139251507783186 2201 2 1.463102243639831 2201 3 1.4715006046211911 2201 4 0.5123273710846945 2201 5 0.9855925640784093 2201 6 0.9655963808534835 2201 7 0.8601791100396738 2201 8 0.8988624812384174 2201 9 1.3371317879327265 2201 10 0.6741117798740177 2201 11 0.9806140177687297 2201 12 0.26802154302305325 2201 13 0.22063739489655632 2201 14 0.8492637209556193 2201 15 0.5689107884380107 2201 16 0.48753800699360283 2201 17 0.608148938101742 2201 18 0.6050042792615002 2201 19 0.9718758585793381 2201 20 1.2082792210453803 2201 21 0.47803204369063224 2201 22 0.13048909138931464 2201 23 0.29028786513742233 2201 24 0.2877660159767714 2201 25 0.8100210052951198 2201 26 0.3883975532640136 2201 27 0.8856189097404723 2201 28 0.7482596468899629 2201 29 0.31266470680292713 2201 30 0.30824398901014405 2201 31 -0.47623472808356837 2201 32 0.5102221150090003 2201 33 -0.33057713051249493 2201 34 0.00536209100694407 2201 35 -0.15767852093622992 2201 36 0.1267428006289535 2201 37 -0.3376278066611178 2201 38 -0.7255501659002519 2201 39 -0.20875072151045515 2201 40 0.36266781056625064 2201 41 0.586012524105378 2201 42 0.886957967483184 2201 43 0.7864714648105249 2201 44 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9.0 22 13.0 23 27.0 24 50.0 25 84.0 26 106.0 27 168.0 28 201.0 29 333.0 30 405.0 31 470.0 32 619.0 33 768.0 34 874.0 35 1155.0 36 1487.0 37 2286.0 38 4104.0 39 13907.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.765801041705146 23.82623471611688 13.556942853976581 22.851021388201396 2 11.878738645594861 25.70711173473156 39.4210176500997 22.993131969573888 3 20.050217856879108 30.75474484897718 30.839672106934497 18.355365187209216 4 12.15936784580164 23.203603869728973 35.63990842626099 28.997119858208404 5 11.860276198212835 36.6110331585555 35.62883095783177 15.899859685399898 6 29.32944391108485 41.381729562070745 14.079462373532238 15.209364153312164 7 18.92770105605199 38.37604312827708 20.936415331216306 21.75984048445462 8 21.084114910272504 37.67077763828373 22.09954951628388 19.145557935159886 9 21.431208921054576 22.63865297983901 21.733993058119786 34.19614504098663 10 14.123772247249095 32.67114688723137 29.56945572705118 23.635625138468356 11 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17.546709991876522 33.801048667011294 34.01890554611919 14.633335794992986 25 15.999556901262832 35.40358909977107 33.2841001403146 15.312753858651504 26 17.184846023188836 33.37271988774832 34.38446200428329 15.057972084779559 27 15.224134111217783 36.29347906358467 33.91182335130345 14.570563473894099 28 13.987150136622112 33.03301085591906 34.354922088472044 18.62491691898678 29 14.950889889963815 33.5056495088989 36.69596041651281 14.847500184624474 30 14.854885163577283 33.19917288235729 36.773502695517315 15.172439258548112 31 17.683332102503506 33.85274351968096 33.154863008640426 15.309061369175097 32 14.064692415626615 33.83797356177535 34.41400192009453 17.683332102503506 33 14.168082120965956 35.23742707333284 36.11254707924083 14.481943726460381 34 17.325160623292224 34.64293626763163 33.5831917879034 14.448711321172736 35 17.17007606528321 35.662063363119415 33.19917288235729 13.968687689240086 36 14.297319252640131 38.28003840189055 33.391182335130345 14.03146001033897 37 14.03884498929178 37.92555941215567 33.16594047706964 14.869655121482902 38 13.798833173325455 34.73524850454176 36.10516210028801 15.360756221844769 39 14.223469463112028 34.44723432538217 33.78258621962927 17.546709991876522 40 14.09053984196145 34.18876006203382 36.651650542795956 15.069049553208774 41 14.529946089653645 34.08537035669448 33.11793811387638 18.266745439775498 42 17.022376486227014 34.55062403072151 34.05952293035965 14.367476552691825 43 13.979765157669299 34.4656967727642 36.40794623735322 15.146591832213277 44 14.153312163060336 37.615390296137655 33.405952293035966 14.825345247766045 45 14.526253600177238 34.16291263569899 35.97223247913743 15.33860128498634 46 17.41747286020235 34.12968023041134 32.95177608743815 15.501070821948156 47 14.23085444206484 34.144450188316966 32.57514216084484 19.049553208773354 48 13.699135957462522 34.51000664648106 32.6157595450853 19.175097850971124 49 16.69374492282697 34.35861457794845 33.25086773502696 15.696772764197622 50 13.60313123107599 34.60601137286759 35.632523447308174 16.158333948748247 51 13.322502030869213 33.93397828816188 33.30625507717303 19.437264603795878 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 862.0 1 486.5 2 111.0 3 182.5 4 254.0 5 255.5 6 257.0 7 280.0 8 303.0 9 260.5 10 218.0 11 380.0 12 542.0 13 911.5 14 1281.0 15 1429.5 16 1578.0 17 1115.5 18 653.0 19 544.5 20 436.0 21 648.0 22 860.0 23 599.5 24 339.0 25 355.0 26 578.0 27 785.0 28 625.0 29 465.0 30 618.5 31 772.0 32 1026.0 33 1280.0 34 1379.5 35 1479.0 36 1465.5 37 1452.0 38 1410.0 39 1368.0 40 1275.5 41 1183.0 42 1126.0 43 1069.0 44 1076.5 45 1084.0 46 1084.0 47 1084.0 48 1061.0 49 1038.0 50 1107.5 51 1177.0 52 1404.5 53 1632.0 54 1228.0 55 824.0 56 738.5 57 653.0 58 557.0 59 461.0 60 445.0 61 429.0 62 360.0 63 291.0 64 245.0 65 199.0 66 155.0 67 111.0 68 95.5 69 80.0 70 57.5 71 35.0 72 34.5 73 34.0 74 23.5 75 10.5 76 8.0 77 8.5 78 9.0 79 5.5 80 2.0 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04061738424045491 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.003692489476404992 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 27082.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.85894071837532 #Duplication Level Percentage of deduplicated Percentage of total 1 95.36512962024618 64.71376677500464 2 3.170829637938069 4.30338280857814 3 0.4796328811441362 0.9764213774443049 4 0.19007272524137023 0.5159253517733674 5 0.12803225248972114 0.4344066515870024 6 0.03391793938406995 0.13809812627718301 7 0.03336392430392353 0.1584828393030616 8 0.02754241614163856 0.14951993473570196 9 0.027393397839650852 0.16729982640681643 >10 0.3646284141809177 5.009757460254164 >50 0.09788552241292738 4.69396822290812 >100 0.06525701494194386 9.756959104849047 >500 0.016314253735485965 8.982011520878434 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 878 3.242005760283583 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 827 3.0536887969869286 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 729 2.6918248282992394 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 432 1.5951554538069568 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 365 1.347758658887822 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 315 1.1631341850675725 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 244 0.9009674322428182 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 209 0.7717303005686434 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.7311129163281885 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 178 0.6572631268000886 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 173 0.6388006794180637 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 148 0.5464884425079389 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 144 0.5317184846023189 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 131 0.483716121409054 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 107 0.3950963739753342 No Hit TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT 100 0.3692489476404992 Illumina PCR Primer Index 8 (95% over 21bp) AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 92 0.3397090318292593 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.31755409497082937 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 86 0.31755409497082937 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.31016911601801933 No Hit CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 82 0.30278413706520935 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 81 0.29909164758880435 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 70 0.25847426334834944 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.25478177387194445 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 68 0.25108928439553946 No Hit TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.2437043054427295 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64 0.2363193264899195 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.2289343475371095 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.1957019422494646 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 53 0.1957019422494646 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.1957019422494646 No Hit ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 52 0.19200945277305959 No Hit GATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51 0.1883169632966546 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 49 0.18093198434384464 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA 48 0.17723949486743962 No Hit ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.17354700539103463 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.16985451591462966 No Hit GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.1403146001033897 No Hit CCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 35 0.12923713167417472 No Hit ACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.12554464219776973 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.12185215272136475 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA 31 0.11446717376855475 No Hit TGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 31 0.11446717376855475 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.11077468429214977 No Hit GTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29 0.10708219481574477 No Hit GATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29 0.10708219481574477 No Hit ATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28 0.10338970533933978 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.003692489476404992 10 0.0 0.0 0.007384978952809984 11 0.0 0.0 0.007384978952809984 12 0.0 0.0 0.007384978952809984 13 0.0 0.0 0.007384978952809984 14 0.0 0.0 0.007384978952809984 15 0.0 0.0 0.007384978952809984 16 0.0 0.0 0.007384978952809984 17 0.0 0.0 0.011077468429214976 18 0.0 0.0 0.011077468429214976 19 0.0 0.0 0.014769957905619968 20 0.0 0.0 0.022154936858429952 21 0.0 0.0 0.022154936858429952 22 0.0 0.0 0.025847426334834944 23 0.0 0.0 0.025847426334834944 24 0.0 0.0 0.03323240528764493 25 0.0 0.0 0.03323240528764493 26 0.0 0.0 0.05907983162247987 27 0.0 0.0 0.08492725795731482 28 0.0 0.0 0.11446717376855475 29 0.0 0.0 0.16616202643822464 30 0.0 0.0 0.20677941067867955 31 0.0 0.0 0.38771139502252416 32 0.0 0.0 0.5354109740787238 33 0.0 0.0 0.6978805110405435 34 0.0 0.0 0.993279669152943 35 0.0 0.0 1.2037515693080274 36 0.0 0.0 1.5360756221844767 37 0.0 0.0 1.894247101395761 38 0.0 0.0 2.304113433276715 39 0.0 0.0 2.821061959973414 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTCT 30 1.11099514E-4 37.49846 45 TGCCGTC 135 0.0 34.99856 45 ATCTCCG 145 0.0 34.136528 10 CCACGAG 145 0.0 34.136528 20 GAGCCCA 145 0.0 34.136528 16 CACGAGA 145 0.0 34.136528 21 TCTCGTA 145 0.0 34.136528 38 ACGAGAC 145 0.0 34.136528 22 CGAGCCC 145 0.0 34.136528 15 CCGAGCC 145 0.0 34.136528 14 TCGTATG 145 0.0 34.136528 40 TCCGAGC 145 0.0 34.136528 13 CTCGTAT 145 0.0 34.136528 39 GTATGCC 140 0.0 33.748615 42 AATCTCG 140 0.0 33.748615 36 TATGCCG 140 0.0 33.748615 43 ATGCCGT 140 0.0 33.748615 44 CGTATGC 140 0.0 33.748615 41 CGAGACT 135 0.0 33.331963 23 AGCCCAC 150 0.0 32.998646 17 >>END_MODULE