##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_8.34100000006899.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 78556 Sequences flagged as poor quality 0 Sequence length 51 %GC 29 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.59348744844442 38.0 38.0 38.0 32.0 38.0 2 36.48009063597943 38.0 38.0 38.0 32.0 38.0 3 36.50621212892713 38.0 38.0 38.0 32.0 38.0 4 36.54401955293039 38.0 38.0 38.0 32.0 38.0 5 36.50089108406742 38.0 38.0 38.0 32.0 38.0 6 38.478588522837214 40.0 38.0 40.0 38.0 40.0 7 38.44214318447986 40.0 38.0 40.0 38.0 40.0 8 38.407861907429094 40.0 38.0 40.0 38.0 40.0 9 38.44000458271806 40.0 38.0 40.0 38.0 40.0 10 38.40787463720149 40.0 38.0 40.0 38.0 40.0 11 38.25785426956566 40.0 38.0 40.0 38.0 40.0 12 38.312121289271346 40.0 38.0 40.0 38.0 40.0 13 38.22739192423239 40.0 38.0 40.0 38.0 40.0 14 38.21736086358776 40.0 38.0 40.0 38.0 40.0 15 38.0730434339834 40.0 38.0 40.0 32.0 40.0 16 38.03672539334997 40.0 38.0 40.0 32.0 40.0 17 38.15259178165894 40.0 38.0 40.0 38.0 40.0 18 38.06355975355161 40.0 38.0 40.0 32.0 40.0 19 38.22378939864555 40.0 38.0 40.0 38.0 40.0 20 38.22025052192067 40.0 38.0 40.0 38.0 40.0 21 38.25882173226743 40.0 38.0 40.0 38.0 40.0 22 38.32070115586333 40.0 38.0 40.0 38.0 40.0 23 38.250432812261316 40.0 38.0 40.0 38.0 40.0 24 38.19216864402465 40.0 38.0 40.0 38.0 40.0 25 38.14918020265797 40.0 38.0 40.0 38.0 40.0 26 37.93766230459799 40.0 38.0 40.0 32.0 40.0 27 37.75133662610113 40.0 38.0 40.0 32.0 40.0 28 37.604167727481034 40.0 38.0 40.0 32.0 40.0 29 37.376992209379296 40.0 38.0 40.0 32.0 40.0 30 37.135432048474975 40.0 38.0 40.0 32.0 40.0 31 36.88449004531799 40.0 38.0 40.0 32.0 40.0 32 36.53659809562605 40.0 38.0 40.0 27.0 40.0 33 36.120423646825195 40.0 38.0 40.0 27.0 40.0 34 35.67718570191965 40.0 38.0 40.0 13.0 40.0 35 35.11314221701716 40.0 38.0 40.0 13.0 40.0 36 34.466851672692094 40.0 32.0 40.0 13.0 40.0 37 33.74885432048475 38.0 32.0 40.0 13.0 40.0 38 32.94411629920057 38.0 27.0 40.0 13.0 40.0 39 32.00052192066806 38.0 27.0 40.0 13.0 40.0 40 31.09864300626305 38.0 27.0 40.0 13.0 40.0 41 30.214725800702684 38.0 13.0 40.0 13.0 40.0 42 29.321146697897042 38.0 13.0 40.0 13.0 40.0 43 28.459761189469933 32.0 13.0 40.0 13.0 40.0 44 27.615013493558735 32.0 13.0 40.0 13.0 40.0 45 26.796259992871327 27.0 13.0 38.0 13.0 40.0 46 26.053655990630887 27.0 13.0 38.0 13.0 40.0 47 25.451104944243596 27.0 13.0 38.0 13.0 40.0 48 24.793115739090585 27.0 13.0 38.0 13.0 40.0 49 24.31726411731758 27.0 13.0 38.0 13.0 40.0 50 23.815469219410357 27.0 13.0 38.0 13.0 40.0 51 22.755753857121036 13.0 13.0 32.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.0801477097450416 2201 2 0.17559034895138126 2201 3 0.5704823798151537 2201 4 0.748362109009058 2201 5 0.1417722250581619 2201 6 0.3816221933953159 2201 7 0.7361743496999509 2201 8 0.7833697853880892 2201 9 0.9900566271407243 2201 10 0.22701536918025056 2201 11 0.3549048673473436 2201 12 0.6665767000485943 2201 13 0.2137940896745505 2201 14 0.7970127193139049 2201 15 0.8774224609015135 2201 16 0.5640129393465969 2201 17 0.9438485933238709 2201 18 0.6920108292523182 2201 19 0.5583764723046514 2201 20 0.7710555073338696 2201 21 0.3819530304055192 2201 22 0.7612575592966664 2201 23 0.5826743898307569 2201 24 1.011869287898655 2201 25 1.064699490617258 2201 26 0.7903446944597405 2201 27 1.0482229290616232 2201 28 0.45686439728765293 2201 29 0.5129807486181193 2201 30 1.2124382822030029 2201 31 0.6419731390181695 2201 32 1.1468568197582982 2201 33 1.322579668422435 2201 34 0.8405287055717068 2201 35 1.7536325365626055 2201 36 0.9920133232517188 2201 37 1.247444095733556 2201 38 1.4271734911355338 2201 39 1.8051666198910397 2201 40 2.245371714090954 2201 41 2.4342063472973656 2201 42 1.391957463526719 2201 43 0.11531821193422331 2201 44 1.270016087246752 2201 45 0.0428974639592532 2201 46 0.5691850868766828 2201 47 0.5428292470536356 2201 48 -0.16698523710761748 2201 49 -0.6112523196607746 2201 50 -1.4404015177738465 2201 51 -0.5558891233654215 1101 1 0.6053428152465372 1101 2 0.7156231074059534 1101 3 0.6020846536372915 1101 4 0.2241304874655583 1101 5 0.6660038466016616 1101 6 0.6800131751854721 1101 7 0.3366175523206252 1101 8 0.5285282784991807 1101 9 0.5035935767505109 1101 10 0.734482369854689 1101 11 0.735384682358422 1101 12 0.5067347114176997 1101 13 0.8958443835371526 1101 14 0.6755173922110984 1101 15 0.9035328761630979 1101 16 1.1146440213390818 1101 17 0.9425960641784812 1101 18 0.9271972633981918 1101 19 0.8915492249783199 1101 20 0.5644845467403599 1101 21 0.9622594183041571 1101 22 1.1225640435436972 1101 23 0.7821155691351223 1101 24 0.8176309219674422 1101 25 0.6629266801345466 1101 26 0.8621242493301509 1101 27 1.001108563515814 1101 28 1.0817800924413277 1101 29 1.2058220628102418 1101 30 1.0581845005284691 1101 31 0.8000808565246729 1101 32 1.0717050710349199 1101 33 1.278259406354799 1101 34 1.2406635933258272 1101 35 1.3143802001140088 1101 36 1.5858084865622502 1101 37 0.8378670651911122 1101 38 1.59587953217995 1101 39 1.4580233498264832 1101 40 1.5094626669765923 1101 41 1.7911771537334396 1101 42 1.9442264682477877 1101 43 1.4213207169925113 1101 44 2.0927350389762047 1101 45 0.26228276119405436 1101 46 0.8829918119947102 1101 47 -0.4739353738154257 1101 48 -0.5245341339184861 1101 49 -0.5420741714769441 1101 50 -0.161569260330932 1101 51 -0.11885280002020338 2202 1 0.3197353386110251 2202 2 -0.06605913558470178 2202 3 0.02862670971206427 2202 4 0.11908375849359487 2202 5 -0.02894942442637216 2202 6 -0.04559430145004484 2202 7 -0.21908338225881607 2202 8 0.10656566167674697 2202 9 0.1449019879654614 2202 10 0.5269122763967431 2202 11 0.24603888796590212 2202 12 0.2562674216980767 2202 13 0.011732234004448117 2202 14 0.3670127193139052 2202 15 0.4568039042004841 2202 16 0.06576551666618258 2202 17 0.400446531468198 2202 18 -0.09149432538685431 2202 19 0.003324925912899346 2202 20 -0.5092537710166525 2202 21 -0.4187686190790174 2202 22 -0.3627630592600397 2202 23 0.05669500838746444 2202 24 0.15351877243473666 2202 25 -0.06426958154769125 2202 26 -0.051614068426857784 2202 27 -0.2759007822785833 2202 28 -0.20653766456801748 2202 29 0.22638281047379394 2202 30 -0.11807718171451853 2202 31 -0.02627428366224649 2202 32 -0.09726689158190283 2202 33 -1.0216471357012722 2202 34 -1.2880279954592169 2202 35 -0.056367463437396736 2202 36 -1.0343784293255993 2202 37 -0.7050301310705649 2202 38 -1.0476718696892036 2202 39 -0.6492663697996832 2202 40 -0.1188550900327563 2202 41 -0.07620602383665087 2202 42 -1.1787641859578173 2202 43 -0.7004549839420662 2202 44 -0.09689112924809251 2202 45 -0.25967985562837725 2202 46 -0.2530829543604298 2202 47 -0.056552196245334585 2202 48 -0.732758432983907 2202 49 0.7589538659062356 2202 50 -2.1268963631346693 2202 51 1.6403995364283936 1102 1 0.629093136776163 1102 2 0.7933904056997036 1102 3 -0.03265769395766682 1102 4 0.3473406392275393 1102 5 0.12077534621582942 1102 6 0.30926808387106064 1102 7 0.5425679938879711 1102 8 0.7033546525024406 1102 9 0.5582964833783066 1102 10 0.630306771809586 1102 11 0.5150297136539734 1102 12 0.4085609996797288 1102 13 0.2062276468484825 1102 14 0.4116916183964747 1102 15 0.35221674823718274 1102 16 0.1759490029047157 1102 17 0.9221896507342535 1102 18 0.7617166837874549 1102 19 0.5948845589404215 1102 20 0.12771870604757396 1102 21 0.16829560110446806 1102 22 0.8290718031252808 1102 23 0.8634839992131518 1102 24 -0.1563894844460023 1102 25 0.42628087716790475 1102 26 0.9115969407474509 1102 27 0.8771267406571965 1102 28 0.701260500569596 1102 29 0.6722543701068204 1102 30 0.9628402494781412 1102 31 0.8410651658790371 1102 32 0.15007255795937624 1102 33 1.1511051578767066 1102 34 0.9834398944490346 1102 35 0.3040912521589334 1102 36 0.5281903780138464 1102 37 0.06405243773676972 1102 38 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23 0.09632463801708724 2203 24 0.0853706242865897 2203 25 0.3675822703041618 2203 26 0.6694970426842559 2203 27 0.6792844029066032 2203 28 0.08420307617272016 2203 29 -0.27176533767435274 2203 30 0.1397005960632569 2203 31 -1.0125705799585418 2203 32 -0.370229854544867 2203 33 -0.6701656542197938 2203 34 -0.4724724399036617 2203 35 -0.02414524121517303 2203 36 1.0104363854892142 2203 37 -0.5850301310705674 2203 38 -0.1973014993188329 2203 39 -1.0648219253552398 2203 40 -1.0158921270697938 2203 41 -1.218428246058874 2203 42 -1.0024678896615207 2203 43 -2.0030475765346587 2203 44 -2.4417059440629068 2203 45 -1.3485687445172658 2203 46 -1.95086073213821 2203 47 -1.5198855295786657 2203 48 -1.0168325070579805 2203 49 -2.3728979859456167 2203 50 -1.629118585356892 2203 51 -1.855526389497534 1103 1 -0.03246805121948171 1103 2 -0.24944896609317624 1103 3 0.28828772666121694 1103 4 0.006880368663090053 1103 5 0.49342345692955547 1103 6 0.3388124782109756 1103 7 -0.44755795852999825 1103 8 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1.8137868368536019 1103 45 0.010659127422471215 1103 46 -0.13918464927568408 1103 47 0.1736172952800885 1103 48 0.0699534314228707 1103 49 -0.16935121883952675 1103 50 0.14818838262804235 1103 51 -0.1457021584868592 2204 1 0.26298320185888713 2204 2 -0.18708477661034095 2204 3 -0.19222799114263722 2204 4 0.31087863028846385 2204 5 0.3741274986505445 2204 6 -0.31191908777482524 2204 7 -0.6100235531989853 2204 8 -0.16993006481897766 2204 9 -0.0279185248550462 2204 10 -0.34052362103915357 2204 11 -0.043191881264867504 2204 12 0.2374640028946544 2204 13 -0.15407973180752066 2204 14 -0.21153428923310713 2204 15 -0.014990967594386007 2204 16 0.16944073034139961 2204 17 -0.28921158818992154 2204 18 -0.3564515903441219 2204 19 -0.5245383219503523 2204 20 -0.21361274537562025 2204 21 0.09294078263038585 2204 22 0.21698053048355348 2204 23 -1.0696297779373225 2204 24 -0.4630624241464645 2204 25 0.056328709050596615 2204 26 0.3831722563594653 2204 27 -0.4567554831332785 2204 28 0.4374794294490769 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1213 34 0.7819720045407834 1213 35 1.1764896794197455 1213 36 0.5770501421029692 1213 37 1.1706841546437161 1213 38 0.8123281303107959 1213 39 1.2707336302003185 1213 40 1.6511449099672468 1213 41 1.913793976163344 1213 42 2.449807242613609 1213 43 2.218116444629363 1213 44 0.8473945850376197 1213 45 1.9417487158001947 1213 46 3.284059902782424 1213 47 3.026304946611809 1213 48 3.152955852730379 1213 49 2.9289538659062337 1213 50 2.1716750654367587 1213 51 2.5375423935712504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 2.0 16 1.0 17 2.0 18 1.0 19 3.0 20 14.0 21 55.0 22 111.0 23 195.0 24 427.0 25 681.0 26 1083.0 27 1623.0 28 2175.0 29 2715.0 30 3642.0 31 4600.0 32 6003.0 33 7241.0 34 7974.0 35 8103.0 36 7541.0 37 6818.0 38 6774.0 39 10771.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.033112582781456 24.959246051961284 11.659449821701477 13.348191543555782 2 8.674287751101154 29.262672811059907 31.841790360770933 30.221249077068002 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9.298062479313593 36.992387402296515 23.259159304427527 30.45039081396237 17 18.549824329140996 32.691328479046796 34.11833596415296 14.64051122765925 18 11.516771688625804 26.492266564827194 46.85379670294698 15.137165043600026 19 16.244462549009626 41.5881664035847 31.5698355313407 10.597535516064973 20 17.588726513569938 24.66520698609909 37.24731401802536 20.498752482305616 21 3.1429808035032334 41.62762869799888 37.14674881613117 18.082641682366717 22 5.548907785528795 43.15010947604257 37.515912215489585 13.78507052293905 23 2.866744742604002 37.21930851876368 44.38362442079536 15.530322317836957 24 5.267579815672896 44.8520800448088 45.22506237588472 4.6552777636335865 25 8.90065685625541 38.96837924537909 42.094811344773156 10.036152553592343 26 4.034167578990249 40.195279680219976 47.728950785446955 8.041601955342822 27 7.566576709608432 42.71475126024747 46.51204236468252 3.2066296654615813 28 2.9418503997148533 41.51560670095218 46.7093538367534 8.83318906257956 29 5.303223178369571 42.62564285350578 47.4260400224044 4.645093945720251 30 4.9648640391078525 42.293003360831044 47.59649658824728 5.1456360118138305 31 6.1013799073272565 43.17429604358674 44.27669433270533 6.44762971638067 32 6.54819491827486 43.29777483578594 44.542746575691226 5.6112836702479765 33 5.490350832527115 45.148683741534704 43.52436478435766 5.836600641580528 34 6.473089261164009 46.34910127806915 42.08335454962066 5.0944549111461885 35 6.563470645144864 46.523499159835026 41.08406741687459 5.828962778145526 36 7.119761698660827 46.36692295941749 40.83456387799786 5.678751463923825 37 8.190335556800244 46.30963898365497 39.146596058862464 6.353429400682316 38 9.30291766383217 44.375986557360356 38.83598961250573 7.4851061663017475 39 10.499516268649117 43.48999439890015 36.57645501298437 9.434034319466369 40 11.65156067009522 42.64982942104995 36.091450684861755 9.607159223993076 41 12.827791639085492 41.80839146596059 33.746626610316206 11.61719028463771 42 14.818728041142624 40.90966953510871 32.58439839095677 11.687204032791893 43 15.375019094658587 39.88746881205764 32.379449055450884 12.358063037832883 44 16.448138907276338 39.704160089617595 30.687662304597996 13.16003869850807 45 18.021538774886707 37.68776414277713 30.431793879525433 13.858903202810735 46 19.938642497072152 36.682112123835225 28.850756148480066 14.528489230612557 47 20.07485106166302 35.74010896685167 28.303375935638268 15.88166403584704 48 20.65278272824482 34.8681195580223 27.822190539233162 16.656907174499718 49 22.101430826416824 33.98849228575793 27.59432761342227 16.315749274402975 50 21.59987779418504 33.08085951423189 28.342838230052447 16.97642446153063 51 22.66807551781645 32.87036447658209 26.456696923018168 18.004863082583288 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14.0 1 15.0 2 16.0 3 45.5 4 75.0 5 83.0 6 91.0 7 85.0 8 79.0 9 105.0 10 131.0 11 182.5 12 234.0 13 419.5 14 605.0 15 950.0 16 1295.0 17 1431.0 18 1567.0 19 1943.5 20 2320.0 21 2975.0 22 3630.0 23 4535.0 24 5440.0 25 5611.0 26 8128.5 27 10475.0 28 11416.0 29 12357.0 30 11109.5 31 9862.0 32 9233.0 33 8604.0 34 7868.0 35 7132.0 36 5767.0 37 4402.0 38 3104.0 39 1806.0 40 1183.0 41 560.0 42 393.5 43 227.0 44 194.5 45 162.0 46 203.5 47 245.0 48 258.0 49 271.0 50 522.5 51 774.0 52 455.5 53 137.0 54 114.5 55 92.0 56 68.5 57 45.0 58 33.0 59 21.0 60 23.0 61 25.0 62 25.0 63 25.0 64 17.5 65 10.0 66 10.0 67 10.0 68 11.5 69 13.0 70 10.0 71 7.0 72 4.5 73 2.0 74 2.5 75 3.5 76 4.0 77 2.5 78 1.0 79 2.0 80 3.0 81 2.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04582718061001069 2 0.0025459544783339276 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0025459544783339276 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0025459544783339276 17 0.0 18 0.0012729772391669638 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0025459544783339276 27 0.0 28 0.0 29 0.0 30 0.005091908956667855 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.006364886195834819 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 78556.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.857054263209875 #Duplication Level Percentage of deduplicated Percentage of total 1 86.24054708814938 44.72180730039069 2 6.447000937978952 6.686449549514791 3 2.199298048540187 3.421473547323603 4 1.31022851768895 2.7177836535600375 5 0.5914527971693406 1.5335499898468883 6 0.49394800664351696 1.5368813150230327 7 0.3858936856586177 1.4007916857920293 8 0.27130593222713306 1.1255301159546545 9 0.20061635487362586 0.9363035880692089 >10 1.6270506627185857 16.155886036704846 >50 0.12844949336466863 4.5935398909892164 >100 0.08482085185837931 7.981500400796467 >500 0.016964170237621964 5.890299512993711 >1k 0.002423452891088852 1.2982034130408584 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 1033 1.3149854880594736 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 910 1.158409287641937 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 729 0.9280004073527165 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA 685 0.8719894088293701 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 636 0.8096135241101889 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 600 0.7637863435001782 RNA PCR Primer, Index 27 (95% over 21bp) AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 569 0.7243240490860023 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 558 0.7103212994551658 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 0.6084831203218086 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA 453 0.5766586893426345 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 452 0.5753857121034676 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 414 0.5270125770151229 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 401 0.5104638729059524 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 282 0.3589795814450838 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 238 0.30296858292173734 No Hit GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAA 176 0.2240439940933856 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGTT 170 0.21640613065838382 No Hit GCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 166 0.21131422170171596 No Hit TTGCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 157 0.1998574265492133 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 155 0.19731147207087937 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGA 150 0.19094658587504454 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTG 149 0.1896736086358776 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAA 148 0.18840063139671065 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAA 144 0.18330872244004276 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAA 144 0.18330872244004276 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAA 132 0.1680329955700392 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG 131 0.16676001833087223 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAA 128 0.16294108661337137 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 128 0.16294108661337137 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTT 125 0.15912215489587045 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAGTACTCTGCGT 122 0.1553032231783696 No Hit TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 116 0.14766535974336778 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAGTACTCTGC 116 0.14766535974336778 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAGTACTCTGCG 114 0.14511940526503386 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT 114 0.14511940526503386 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATA 113 0.1438464280258669 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGT 112 0.14257345078669995 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAGTACTCTGCG 108 0.13748154183003208 No Hit GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA 104 0.13238963287336422 No Hit TTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 104 0.13238963287336422 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAGTACTCTG 103 0.13111665563419725 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA 102 0.1298436783950303 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGCCAAAAAAAAAAAAAAAAA 102 0.1298436783950303 No Hit TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT 99 0.12602474667752941 RNA PCR Primer, Index 38 (95% over 21bp) AAAGTACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAA 98 0.12475176943836244 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA 97 0.12347879219919548 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95 0.12093283772086154 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAA 95 0.12093283772086154 No Hit CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 93 0.11838688324252764 No Hit GCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 89 0.11329497428585976 No Hit ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAA 87 0.11074901980752586 No Hit TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 85 0.10820306532919191 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 85 0.10820306532919191 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82 0.10438413361169101 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAA 80 0.1018381791333571 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 80 0.1018381791333571 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0012729772391669638 11 0.0 0.0 0.0025459544783339276 12 0.0 0.0 0.0025459544783339276 13 0.0 0.0 0.003818931717500891 14 0.0 0.0 0.005091908956667855 15 0.0 0.0 0.006364886195834818 16 0.0 0.0 0.006364886195834818 17 0.0 0.0 0.006364886195834818 18 0.0 0.0 0.006364886195834818 19 0.0 0.0 0.007637863435001782 20 0.0 0.0 0.01018381791333571 21 0.0 0.0 0.01018381791333571 22 0.0 0.0 0.011456795152502672 23 0.0 0.0 0.0140027496308366 24 0.0 0.0 0.015275726870003564 25 0.0 0.0 0.017821681348337493 26 0.0 0.0 0.021640613065838383 27 0.0 0.0 0.022913590305005345 28 0.0 0.0 0.03182443097917409 29 0.0 0.0 0.036916339935841945 30 0.0 0.0 0.04073527165334284 31 0.0 0.0 0.05855695300168033 32 0.0 0.0 0.06492183919751515 33 0.0 0.0 0.06874077091501604 34 0.0 0.0 0.09547329293752228 35 0.0 0.0 0.10565711085085798 36 0.0 0.0 0.12093283772086155 37 0.0 0.0 0.1362085645908651 38 0.0 0.0 0.15912215489587045 39 0.0 0.0 0.1896736086358776 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAGATA 40 3.404457E-7 39.373886 27 TAAATCT 40 3.404457E-7 39.373886 34 TATAAAT 40 3.404457E-7 39.373886 32 ATAAATC 40 3.404457E-7 39.373886 33 CAGATAT 40 3.404457E-7 39.373886 28 CGAGACA 40 3.404457E-7 39.373886 23 AGATATA 40 3.404457E-7 39.373886 29 CTTATAC 95 0.0 37.9178 1 TATACAC 95 0.0 37.893665 3 TTATACA 95 0.0 37.893665 2 TATCTCG 55 2.6811904E-9 36.81714 36 CTATCTC 55 2.6811904E-9 36.81714 35 GGTTGAA 25 0.0020889498 36.02191 45 TGCCGTC 100 0.0 36.02191 45 GTATGCC 100 0.0 35.99898 42 ATCTCGT 100 0.0 35.99898 37 ATCTCCG 100 0.0 35.99898 10 CACATCT 100 0.0 35.99898 7 TATGCCG 100 0.0 35.99898 43 CATCTCC 100 0.0 35.99898 9 >>END_MODULE