FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_8.34100000006899.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_8.34100000006899.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78556
Sequences flagged as poor quality0
Sequence length51
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA10331.3149854880594736No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA9101.158409287641937No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.9280004073527165No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA6850.8719894088293701No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6360.8096135241101889No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC6000.7637863435001782RNA PCR Primer, Index 27 (95% over 21bp)
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA5690.7243240490860023No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.7103212994551658No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.6084831203218086No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA4530.5766586893426345No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.5753857121034676No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4140.5270125770151229No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4010.5104638729059524No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA2820.3589795814450838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.30296858292173734No Hit
GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAA1760.2240439940933856No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGTT1700.21640613065838382No Hit
GCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1660.21131422170171596No Hit
TTGCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1570.1998574265492133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.19731147207087937No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGA1500.19094658587504454No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTG1490.1896736086358776No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAA1480.18840063139671065No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAA1440.18330872244004276No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAA1440.18330872244004276No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAA1320.1680329955700392No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG1310.16676001833087223No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAA1280.16294108661337137No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.16294108661337137No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTT1250.15912215489587045No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAGTACTCTGCGT1220.1553032231783696No Hit
TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1160.14766535974336778No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAGTACTCTGC1160.14766535974336778No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAGTACTCTGCG1140.14511940526503386No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT1140.14511940526503386No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATA1130.1438464280258669No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGT1120.14257345078669995No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAGTACTCTGCG1080.13748154183003208No Hit
GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA1040.13238963287336422No Hit
TTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT1040.13238963287336422No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAGTACTCTG1030.13111665563419725No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA1020.1298436783950303No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGCCAAAAAAAAAAAAAAAAA1020.1298436783950303No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT990.12602474667752941RNA PCR Primer, Index 38 (95% over 21bp)
AAAGTACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAA980.12475176943836244No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA970.12347879219919548No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.12093283772086154No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAA950.12093283772086154No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC930.11838688324252764No Hit
GCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.11329497428585976No Hit
ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAA870.11074901980752586No Hit
TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT850.10820306532919191No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.10820306532919191No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.10438413361169101No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAA800.1018381791333571No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.1018381791333571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGATA403.404457E-739.37388627
TAAATCT403.404457E-739.37388634
TATAAAT403.404457E-739.37388632
ATAAATC403.404457E-739.37388633
CAGATAT403.404457E-739.37388628
CGAGACA403.404457E-739.37388623
AGATATA403.404457E-739.37388629
CTTATAC950.037.91781
TATACAC950.037.8936653
TTATACA950.037.8936652
TATCTCG552.6811904E-936.8171436
CTATCTC552.6811904E-936.8171435
GGTTGAA250.002088949836.0219145
TGCCGTC1000.036.0219145
GTATGCC1000.035.9989842
ATCTCGT1000.035.9989837
ATCTCCG1000.035.9989810
CACATCT1000.035.998987
TATGCCG1000.035.9989843
CATCTCC1000.035.998989
Produced by FastQC (version 0.11.2)