##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_79.34100000006d00.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55249 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.314340531050334 38.0 38.0 38.0 32.0 38.0 2 36.37564480805082 38.0 38.0 38.0 32.0 38.0 3 36.30585168962334 38.0 38.0 38.0 32.0 38.0 4 36.40449600897754 38.0 38.0 38.0 32.0 38.0 5 36.46283190645985 38.0 38.0 38.0 32.0 38.0 6 38.33524588680338 40.0 38.0 40.0 38.0 40.0 7 38.213687125558835 40.0 38.0 40.0 38.0 40.0 8 38.209524154283336 40.0 38.0 40.0 38.0 40.0 9 38.20203080598744 40.0 38.0 40.0 38.0 40.0 10 38.13183949030752 40.0 38.0 40.0 38.0 40.0 11 38.13935093847852 40.0 38.0 40.0 38.0 40.0 12 38.06912342304838 40.0 38.0 40.0 38.0 40.0 13 38.10988434179804 40.0 38.0 40.0 38.0 40.0 14 38.046751977411354 40.0 38.0 40.0 38.0 40.0 15 38.07875255660736 40.0 38.0 40.0 38.0 40.0 16 38.01589169034734 40.0 38.0 40.0 32.0 40.0 17 38.04152111350432 40.0 38.0 40.0 32.0 40.0 18 38.01936686636862 40.0 38.0 40.0 32.0 40.0 19 38.099205415482636 40.0 38.0 40.0 38.0 40.0 20 38.08099694112111 40.0 38.0 40.0 38.0 40.0 21 38.09725062897066 40.0 38.0 40.0 38.0 40.0 22 38.06427265651867 40.0 38.0 40.0 38.0 40.0 23 38.06054408224583 40.0 38.0 40.0 32.0 40.0 24 38.04438089377183 40.0 38.0 40.0 32.0 40.0 25 38.018425672862854 40.0 38.0 40.0 32.0 40.0 26 37.97686835960832 40.0 38.0 40.0 32.0 40.0 27 37.95722999511303 40.0 38.0 40.0 32.0 40.0 28 37.91098481420478 40.0 38.0 40.0 32.0 40.0 29 37.88448659704248 40.0 38.0 40.0 32.0 40.0 30 37.88298430740828 40.0 38.0 40.0 32.0 40.0 31 37.847255153939436 40.0 38.0 40.0 32.0 40.0 32 37.82968017520679 40.0 38.0 40.0 32.0 40.0 33 37.7791996235226 40.0 38.0 40.0 32.0 40.0 34 37.79943528389654 40.0 38.0 40.0 32.0 40.0 35 37.784991583558075 40.0 38.0 40.0 32.0 40.0 36 37.753244402613625 40.0 38.0 40.0 32.0 40.0 37 37.742963673550655 40.0 38.0 40.0 32.0 40.0 38 37.73708121413962 40.0 38.0 40.0 32.0 40.0 39 37.69916197578237 40.0 38.0 40.0 32.0 40.0 40 37.660500642545564 40.0 38.0 40.0 32.0 40.0 41 37.658147658781154 40.0 38.0 40.0 32.0 40.0 42 37.66095313942334 40.0 38.0 40.0 32.0 40.0 43 37.656880667523396 40.0 38.0 40.0 32.0 40.0 44 37.645025249325776 40.0 38.0 40.0 32.0 40.0 45 37.62773986859491 40.0 38.0 40.0 32.0 40.0 46 37.56963926948904 40.0 38.0 40.0 32.0 40.0 47 37.58826404097812 40.0 38.0 40.0 32.0 40.0 48 37.52625386884831 40.0 38.0 40.0 32.0 40.0 49 37.50279643070463 40.0 38.0 40.0 32.0 40.0 50 37.4535828702782 40.0 38.0 40.0 32.0 40.0 51 36.96854241705732 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.7877533529478669 2201 2 0.8128640228143098 2201 3 0.7660509949934422 2201 4 0.6438990162923943 2201 5 0.6358116696660545 2201 6 0.6994823055341399 2201 7 0.617697668523455 2201 8 0.825506601052183 2201 9 1.0781181169729663 2201 10 1.1160294973866627 2201 11 1.1296342289420664 2201 12 0.9785484688247337 2201 13 0.6917892543586817 2201 14 0.2734747709688321 2201 15 0.8798102760180626 2201 16 0.6203785841385212 2201 17 0.0869196311550553 2201 18 0.696614747897847 2201 19 0.28126124336017 2201 20 0.2695308195759125 2201 21 0.48849482582235026 2201 22 0.226469224060601 2201 23 0.5116762802255224 2201 24 0.5739449967108925 2201 25 0.24581108328018786 2201 26 0.5725226852226584 2201 27 0.2220035879524005 2201 28 0.13595977658245317 2201 29 0.7997231876677446 2201 30 0.7042718304491231 2201 31 0.8279670233640815 2201 32 1.0195306230484888 2201 33 1.1942162289448746 2201 34 0.9150720495147198 2201 35 0.4898385052547525 2201 36 0.7998641336523917 2201 37 1.1438070244892913 2201 38 1.4081324302406344 2201 39 0.17575088355944501 2201 40 1.1084463323024494 2201 41 0.861995825099406 2201 42 0.3829801584634964 2201 43 0.6664020108165545 2201 44 0.4321519933848563 2201 45 0.5354765258682406 2201 46 0.40602136004841327 2201 47 0.3594057936427504 2201 48 1.0542774289512522 2201 49 0.7632765917642388 2201 50 0.9298543166724969 2201 51 0.3789524514943494 1101 1 -0.2615644178888914 1101 2 -0.20883579635385985 1101 3 0.13231763974109612 1101 4 -0.3168111596076528 1101 5 -0.011237483712214669 1101 6 0.18230328202599821 1101 7 0.4059600390643041 1101 8 0.7752024747986894 1101 9 0.10968149030848906 1101 10 -0.3452560525064854 1101 11 0.14886815956840138 1101 12 0.36848764357403496 1101 13 -0.02677066673288664 1101 14 0.03592750810511092 1101 15 -0.1721377759299898 1101 16 -0.506368086911948 1101 17 0.012614189761002592 1101 18 0.7160130710936414 1101 19 0.538864399697502 1101 20 0.7985461080848708 1101 21 0.2705102787238758 1101 22 0.2483334357982372 1101 23 0.20311142735687326 1101 24 0.23710462189583836 1101 25 0.10985842834018911 1101 26 0.8444279951026488 1101 27 0.48503169908178023 1101 28 -0.2153471443447259 1101 29 0.24407630290693305 1101 30 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34 0.02374174141822749 1213 35 -1.22953097169772 1213 36 -1.0932574331018472 1213 37 -2.191636013485393 1213 38 -1.4858250572205591 1213 39 -1.3828829831709157 1213 40 -1.0475125881656666 1213 41 0.11659338898763139 1213 42 -0.6989400708176063 1213 43 -0.49266175799166234 1213 44 -0.46753752178113217 1213 45 -1.0131262923787148 1213 46 -1.3042724063714601 1213 47 -1.2853279059034648 1213 48 -0.7015143073754757 1213 49 -0.5718081944788906 1213 50 -0.7324337177196725 1213 51 -1.7364046060647595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 1.0 16 0.0 17 1.0 18 1.0 19 3.0 20 3.0 21 5.0 22 20.0 23 33.0 24 81.0 25 135.0 26 196.0 27 323.0 28 431.0 29 603.0 30 824.0 31 998.0 32 1222.0 33 1526.0 34 1832.0 35 2377.0 36 3042.0 37 4674.0 38 8782.0 39 28135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.99665043182271 22.95450019011063 14.629465336495809 27.419384041570854 2 12.45112945264987 24.09860990443093 43.14364320880394 20.306617434115264 3 16.98492280403265 26.79867508914188 33.16259117812087 23.05381092870459 4 12.675342540136473 23.05924089123785 36.5364078987855 27.729008669840177 5 11.569440170862821 37.950008144943794 38.27942587196148 12.201125812231895 6 27.14257271624826 43.05598291371789 17.260040905717748 12.541403464316096 7 22.50176473782331 34.038625133486576 23.946134771670074 19.513475357020035 8 19.8845227967927 43.11933247660591 21.406722293616173 15.589422432985211 9 21.745189958189286 19.404876106354866 22.054697822585023 36.795236112870825 10 13.679885608789299 33.20603087838694 34.10016470886351 19.01391880396025 11 28.470839517793145 25.24888679723419 27.893422148209822 18.386851536762844 12 20.16507086101106 26.380567974080975 31.989719270937027 21.46464189397093 13 25.366974967872725 23.940704809136818 29.924523520787705 20.767796702202755 14 17.153251642563667 25.47195424351572 28.36250429872034 29.012289815200276 15 20.308059874386867 33.25490054118627 24.451121287263117 21.98591829716375 16 20.88258185964849 32.166452477057575 29.130993538110665 17.819972125183266 17 17.817517059132292 34.753570200365616 30.299190935582544 17.129721804919544 18 21.25830331770711 29.741714782168003 31.859400170138823 17.140581729986064 19 18.796720302629915 30.86933700157469 29.33084761715144 21.00309507864396 20 18.175894586327352 30.1218121594961 30.42046009882532 21.28183315535123 21 16.912523303589204 30.612318775000453 29.544426143459614 22.930731777950733 22 18.257344024326233 34.53999167405745 28.988759977556157 18.213904324060167 23 17.171351517674527 30.306430885626888 29.917283570743365 22.60493402595522 24 21.319842893084036 30.804177451175583 30.42046009882532 17.455519556915057 25 18.27725388694818 32.98521240203443 30.297380948071456 18.440152762945935 26 20.492678600517657 30.641278575177832 30.706438125576934 18.15960469872758 27 17.71434777100038 33.05942188998896 30.51819942442397 18.70803091458669 28 16.89623341598943 30.056652609096997 30.302810910604716 22.74430306430886 29 18.463682600590055 29.176998678709115 33.30739017900777 19.051928541693062 30 17.330075119920355 28.9926690198208 33.99583672730564 19.68141913295321 31 21.779579720899925 28.632192437872177 30.440369961447267 19.14785787978063 32 17.390360006515955 29.526326268348747 30.80055747615341 22.282756248981883 33 17.779507321399482 30.024072833897446 34.25220365979475 17.944216184908324 34 21.12436424188673 29.019529765244616 31.419573204944882 18.436532787923763 35 20.516208438161776 30.049412659052656 30.315480823182323 19.118898079603248 36 17.2401310430958 31.812340494850584 31.314593929301886 19.632934532751726 37 16.648265126970625 31.356223642056868 30.668428387844127 21.327082843128384 38 17.04284240438741 28.05118644681352 34.01871527086463 20.887255877934443 39 17.428369744248766 26.449347499502252 32.653984687505655 23.468298068743326 40 17.689007945845173 25.795942008000143 35.45041539213379 21.064634654020885 41 17.707107820956036 24.968777715433763 33.49019891762747 23.833915545982734 42 19.66732429546236 25.96065087150899 33.55535846802657 20.81666636500208 43 15.493493094897644 26.338938261325996 36.136400658835456 22.031167984940904 44 15.136925555213669 29.381527267461856 33.81056670708972 21.670980470234756 45 15.243714818367755 26.52717696247896 35.62779416822024 22.60131405093305 46 18.043765498018065 25.488244131115493 33.558978443048744 22.909011927817698 47 15.790331046715778 26.25205886079386 32.632264837372624 25.32534525511774 48 13.846404459809229 26.95614400260638 31.62591178120871 27.571539756375678 49 18.096255135839563 25.980560734130936 32.23587757244475 23.687306557584755 50 13.448207207370269 27.607739506597404 34.46940216112509 24.474651124907236 51 12.97399047946569 26.896414414740537 31.452152980144437 28.677442125649332 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 253.0 1 135.5 2 18.0 3 27.5 4 37.0 5 43.0 6 49.0 7 40.0 8 31.0 9 33.5 10 36.0 11 68.0 12 100.0 13 157.5 14 215.0 15 256.5 16 298.0 17 216.0 18 134.0 19 118.5 20 103.0 21 120.0 22 137.0 23 104.5 24 72.0 25 93.0 26 160.0 27 206.0 28 397.5 29 589.0 30 1199.0 31 1809.0 32 3025.0 33 4241.0 34 5952.5 35 7664.0 36 8743.5 37 9823.0 38 9288.0 39 8753.0 40 7389.5 41 6026.0 42 4644.5 43 3263.0 44 2740.5 45 2218.0 46 1704.5 47 1191.0 48 1084.0 49 977.0 50 1087.5 51 1198.0 52 1909.5 53 2621.0 54 1671.5 55 722.0 56 653.0 57 584.0 58 543.5 59 503.0 60 457.0 61 411.0 62 356.5 63 302.0 64 245.5 65 189.0 66 159.5 67 130.0 68 110.5 69 91.0 70 74.0 71 57.0 72 48.0 73 39.0 74 28.5 75 16.0 76 14.0 77 11.0 78 8.0 79 6.0 80 4.0 81 2.0 82 0.0 83 0.0 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032579775199551124 2 0.0018099875110861737 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.005429962533258521 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0036199750221723474 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.007239950044344695 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 55249.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.73657395037901 #Duplication Level Percentage of deduplicated Percentage of total 1 92.75567336031246 76.74286628292691 2 5.2216115399101914 8.64036498623864 3 1.0021822859994205 2.4875138645205284 4 0.32157930979044247 1.0642548138155512 5 0.16122835584201292 0.6669740893010362 6 0.11766220165768951 0.5840980469169501 7 0.09278716740834407 0.537382463654866 8 0.03767464334831712 0.24936567323537553 9 0.05779785329012592 0.43037967266204935 >10 0.19482648001730765 2.730158984345913 >50 0.026101486361447306 1.511674991773252 >100 0.00870025285295867 1.1949163277577648 >500 0.0 0.0 >1k 0.002175063209344703 3.1600498028511947 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 1756 3.178338069467321 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 244 0.44163695270502634 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 176 0.31855780195116656 No Hit TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT 136 0.24615830150771958 Illumina PCR Primer Index 8 (95% over 21bp) CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 108 0.19547865119730673 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT 100 0.18099875110861735 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 94 0.17013882604210032 No Hit GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC 94 0.17013882604210032 No Hit AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC 70 0.12669912577603215 No Hit GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA 67 0.12126916324277362 No Hit AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC 65 0.11764918822060129 No Hit GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC 61 0.11040923817625659 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 61 0.11040923817625659 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61 0.11040923817625659 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60 0.10859925066517041 No Hit AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA 56 0.10135930062082571 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0018099875110861735 3 0.0 0.0 0.0018099875110861735 4 0.0 0.0 0.0018099875110861735 5 0.0 0.0 0.0018099875110861735 6 0.0 0.0 0.0018099875110861735 7 0.0 0.0 0.0018099875110861735 8 0.0 0.0 0.0018099875110861735 9 0.0 0.0 0.007239950044344694 10 0.0 0.0 0.009049937555430867 11 0.0 0.0 0.009049937555430867 12 0.0 0.0 0.009049937555430867 13 0.0 0.0 0.010859925066517041 14 0.0 0.0 0.010859925066517041 15 0.0 0.0 0.012669912577603214 16 0.0 0.0 0.018099875110861734 17 0.0 0.0 0.01990986262194791 18 0.0 0.0 0.023529837644120255 19 0.0 0.0 0.025339825155206427 20 0.0 0.0 0.025339825155206427 21 0.0 0.0 0.06334956288801608 22 0.0 0.0 0.14660898839798006 23 0.0 0.0 0.4144871400387337 24 0.0 0.0 0.7384749045231588 25 0.0 0.0 0.8362142301218122 26 0.0 0.0 1.2108816449166502 27 0.0 0.0 1.3375807706926823 28 0.0 0.0 1.5583992470451953 29 0.0 0.0 1.8805770240185342 30 0.0 0.0 2.2027548009918734 31 0.0 0.0 3.209107857155786 32 0.0 0.0 4.179261163097975 33 0.0 0.0 5.225433944505783 34 0.0 0.0 6.5992144654201885 35 0.0 0.0 7.844485873047476 36 0.0 0.0 9.665333309200166 37 0.0 0.0 11.623739796195407 38 0.0 0.0 13.75047512172166 39 0.0 0.0 16.058209198356533 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTGG 20 6.975019E-4 45.000004 7 GCCGTCT 20 6.975019E-4 45.000004 45 ACGTGGA 20 6.975019E-4 45.000004 8 GGACGTG 40 3.379173E-7 39.375004 6 TGCCGTC 195 0.0 39.23077 45 GTAGGAC 65 9.094947E-12 38.07692 3 ATGCCGT 205 0.0 37.317074 44 GACTTGC 200 0.0 37.125 26 CCACGAG 215 0.0 36.627907 20 TATGCCG 215 0.0 36.627907 43 CCCACGA 215 0.0 36.627907 19 ACGAGAC 215 0.0 36.627907 22 TCGTATG 215 0.0 36.627907 40 ACTTGCT 210 0.0 36.42857 27 CGAGACT 210 0.0 36.42857 23 AATCTCG 205 0.0 36.219513 36 TTGCTGG 205 0.0 36.219513 29 GACATGG 25 0.0020898157 36.0 7 CGTGGAA 25 0.0020898157 36.0 9 TCTCGTA 220 0.0 35.795456 38 >>END_MODULE