FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_79.34100000006d00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_79.34100000006d00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55249
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC17563.178338069467321No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.44163695270502634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.31855780195116656No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT1360.24615830150771958Illumina PCR Primer Index 8 (95% over 21bp)
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1080.19547865119730673No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT1000.18099875110861735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.17013882604210032No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC940.17013882604210032No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC700.12669912577603215No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA670.12126916324277362No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC650.11764918822060129No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC610.11040923817625659No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT610.11040923817625659No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.11040923817625659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.10859925066517041No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA560.10135930062082571No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGG206.975019E-445.0000047
GCCGTCT206.975019E-445.00000445
ACGTGGA206.975019E-445.0000048
GGACGTG403.379173E-739.3750046
TGCCGTC1950.039.2307745
GTAGGAC659.094947E-1238.076923
ATGCCGT2050.037.31707444
GACTTGC2000.037.12526
CCACGAG2150.036.62790720
TATGCCG2150.036.62790743
CCCACGA2150.036.62790719
ACGAGAC2150.036.62790722
TCGTATG2150.036.62790740
ACTTGCT2100.036.4285727
CGAGACT2100.036.4285723
AATCTCG2050.036.21951336
TTGCTGG2050.036.21951329
GACATGG250.002089815736.07
CGTGGAA250.002089815736.09
TCTCGTA2200.035.79545638
Produced by FastQC (version 0.11.2)