FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_78.34100000006cf2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_78.34100000006cf2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97449
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC9200.9440835719196707No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA3320.3406910281275334No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT1990.20420938131740707No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC1490.15290049153916407No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC800.042.205813
CCGAGCC800.042.205814
ATCTCGT750.041.99664737
GACTTGC750.041.99664726
CCCACGA900.039.99680719
CTCCGAG850.039.72310312
CGAGCCC850.039.72310315
CACGAGA850.039.70271321
ATGCCGT850.039.70271344
CTCGTAT800.039.37185739
TCTCGTA800.039.37185738
CGAGACT800.039.37185723
GCCCACG900.037.51626618
CGTATGC900.037.49700541
TATGCCG900.037.49700543
ACGAGAC900.037.49700522
CCACGAG900.037.49700520
TGCCGTC900.037.49700545
TTGCTGG850.037.05586629
CCAACGA250.002098496835.99712429
Produced by FastQC (version 0.11.2)