##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_77.34100000006ce5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 71206 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.24063983372188 38.0 38.0 38.0 32.0 38.0 2 36.30625228211106 38.0 38.0 38.0 32.0 38.0 3 36.235232985984325 38.0 38.0 38.0 32.0 38.0 4 36.35454877397972 38.0 38.0 38.0 32.0 38.0 5 36.40955818329916 38.0 38.0 38.0 32.0 38.0 6 38.30351374884139 40.0 38.0 40.0 38.0 40.0 7 38.18849535151532 40.0 38.0 40.0 38.0 40.0 8 38.123992360194364 40.0 38.0 40.0 38.0 40.0 9 38.163750245765804 40.0 38.0 40.0 38.0 40.0 10 38.09759009072269 40.0 38.0 40.0 38.0 40.0 11 38.112406257899615 40.0 38.0 40.0 38.0 40.0 12 38.03103671038957 40.0 38.0 40.0 32.0 40.0 13 38.06521922309918 40.0 38.0 40.0 38.0 40.0 14 37.96452546133753 40.0 38.0 40.0 32.0 40.0 15 38.00499957868719 40.0 38.0 40.0 32.0 40.0 16 37.99361008903744 40.0 38.0 40.0 32.0 40.0 17 37.9829087436452 40.0 38.0 40.0 32.0 40.0 18 37.97986124764767 40.0 38.0 40.0 32.0 40.0 19 38.03686487093784 40.0 38.0 40.0 32.0 40.0 20 38.00530854141505 40.0 38.0 40.0 32.0 40.0 21 38.03165463584529 40.0 38.0 40.0 32.0 40.0 22 38.029969384602424 40.0 38.0 40.0 32.0 40.0 23 37.998118136112126 40.0 38.0 40.0 32.0 40.0 24 38.030713703901355 40.0 38.0 40.0 32.0 40.0 25 37.974356093587616 40.0 38.0 40.0 32.0 40.0 26 37.93577788388619 40.0 38.0 40.0 32.0 40.0 27 37.890556975535766 40.0 38.0 40.0 32.0 40.0 28 37.869505378760216 40.0 38.0 40.0 32.0 40.0 29 37.89496671628795 40.0 38.0 40.0 32.0 40.0 30 37.875361626829196 40.0 38.0 40.0 32.0 40.0 31 37.8403224447378 40.0 38.0 40.0 32.0 40.0 32 37.846585961857144 40.0 38.0 40.0 32.0 40.0 33 37.7905934893127 40.0 38.0 40.0 32.0 40.0 34 37.80920147178608 40.0 38.0 40.0 32.0 40.0 35 37.831868101002726 40.0 38.0 40.0 32.0 40.0 36 37.76833412914642 40.0 38.0 40.0 32.0 40.0 37 37.76789877257534 40.0 38.0 40.0 32.0 40.0 38 37.776395247591495 40.0 38.0 40.0 32.0 40.0 39 37.73718506867399 40.0 38.0 40.0 32.0 40.0 40 37.69199224784428 40.0 38.0 40.0 32.0 40.0 41 37.73201696486251 40.0 38.0 40.0 32.0 40.0 42 37.71317023846305 40.0 38.0 40.0 32.0 40.0 43 37.75367244333343 40.0 38.0 40.0 32.0 40.0 44 37.70456141336404 40.0 38.0 40.0 32.0 40.0 45 37.740948796449736 40.0 38.0 40.0 32.0 40.0 46 37.655576777237876 40.0 38.0 40.0 32.0 40.0 47 37.71176586242732 40.0 38.0 40.0 32.0 40.0 48 37.67300508384125 40.0 38.0 40.0 32.0 40.0 49 37.63278375417802 40.0 38.0 40.0 32.0 40.0 50 37.61598741679072 40.0 38.0 40.0 32.0 40.0 51 37.122587984158635 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.05174169941648188 2201 2 -0.459145116946857 2201 3 0.28780443776098963 2201 4 0.20802405540592872 2201 5 -0.5698228032368817 2201 6 0.21487944038991458 2201 7 0.19901378637443656 2201 8 -0.28787172828321417 2201 9 0.04603748792644069 2201 10 -0.27080136378413755 2201 11 -0.19527416315568047 2201 12 -0.17350201248321895 2201 13 -0.19857420624786926 2201 14 -0.2775023844989448 2201 15 -0.7904420887867758 2201 16 -0.576609914789934 2201 17 -0.6679239310652676 2201 18 -0.1861353091025748 2201 19 -0.48354780751029836 2201 20 -0.37242839388066784 2201 21 -0.1541151372902334 2201 22 0.2310320131735395 2201 23 -0.11566618520561178 2201 24 0.449905032664347 2201 25 0.016364558779898175 2201 26 -0.561190707571555 2201 27 0.010460166572698881 2201 28 -0.25116644559780354 2201 29 -0.2823858195447002 2201 30 -0.26804636355647915 2201 31 0.14448788168830617 2201 32 -0.5543593419300734 2201 33 -0.2856294065932943 2201 34 -0.14949022926439426 2201 35 -0.3244421444084651 2201 36 0.03557695980775577 2201 37 0.41181895123679624 2201 38 -0.28582401906288624 2201 39 0.7299750951054591 2201 40 -0.08494159451183947 2201 41 0.4755563280258315 2201 42 0.7173548274038524 2201 43 -0.5567250903719767 2201 44 -0.7210616865571211 2201 45 0.12745343510006535 2201 46 -0.7653839522691328 2201 47 0.2544941491080337 2201 48 0.345384721498597 2201 49 0.24459534230150837 2201 50 -0.8104330275216896 2201 51 -0.2719915388488303 1101 1 0.43451397664420455 1101 2 0.7895677543402684 1101 3 -0.35675001768455417 1101 4 0.6534695999603883 1101 5 0.1390880878522296 1101 6 0.29062201464734017 1101 7 0.5034692319189915 1101 8 0.8310391628058937 1101 9 0.24791867604525208 1101 10 0.267911507502987 1101 11 0.45432979724035505 1101 12 0.1955078885068815 1101 13 0.5508317343461897 1101 14 0.6298243481743242 1101 15 0.6820331587379727 1101 16 0.7586376099625411 1101 17 0.4749473560634456 1101 18 0.67257756218455 1101 19 0.3321947667471292 1101 20 0.6145022991886364 1101 21 0.05489476369986335 1101 22 0.014398349807201782 1101 23 0.5341357949924088 1101 24 0.8646575079118719 1101 25 0.5869586181858395 1101 26 -0.5368342719279866 1101 27 -1.0202329027342358 1101 28 0.015368207867545891 1101 29 -0.40614819578232186 1101 30 0.23838928000787263 1101 31 0.7054779806982054 1101 32 0.6090069947035914 1101 33 1.1900141577631373 1101 34 0.8783315529138278 1101 35 0.361894489254901 1101 36 1.0245868607978537 1101 37 0.7942941987615484 1101 38 0.5405126146004804 1101 39 0.7651236099569445 1101 40 0.8815930589535057 1101 41 0.018130585451572756 1101 42 -0.6979917072496136 1101 43 0.5354531274498058 1101 44 0.6886412837399121 1101 45 0.47695838559511117 1101 46 -0.005779991873097856 1101 47 0.815286228315955 1101 48 0.5663748205085 1101 49 0.7839022729945739 1101 50 1.209071922973358 1101 51 0.6378104413491883 2202 1 0.7456678227980547 2202 2 0.6599523697248841 2202 3 0.8201730592385275 2202 4 0.28846959996038635 2202 5 0.962165010929148 2202 6 1.121391245416575 2202 7 1.2150076934574514 2202 8 1.099885316652049 2202 9 1.2040725221990982 2202 10 0.7521422767337569 2202 11 0.9789451818557424 2202 12 0.9351232731222652 2202 13 0.9666009651154255 2202 14 0.7494397327897104 2202 15 1.2793408510456672 2202 16 0.802483763808695 2202 17 1.4295627406788327 2202 18 0.7502698698768597 2202 19 1.0575793821317419 2202 20 1.17488691457325 2202 21 1.0506639944690974 2202 22 1.2213214267302845 2202 23 1.0291357949924063 2202 24 1.1092728925272581 2202 25 1.206958618185837 2202 26 1.070473420379706 2202 27 1.185536328034999 2202 28 1.268445130944464 2202 29 0.9496210349869116 2202 30 1.1364662030847938 2202 31 1.4685549037751287 2202 32 1.4070839177805112 2202 33 1.2750141577631382 2202 34 1.1164084759907453 2202 35 1.3303560277164408 2202 36 1.057663783874773 2202 37 0.9816018910692392 2202 38 1.022820306908173 2202 39 1.0420466868800276 2202 40 1.0019776743381215 2202 41 1.0642844316054223 2202 42 1.175085215827309 2202 43 1.1004531274498035 2202 44 0.9047951298937562 2202 45 0.2923430009797272 2202 46 0.6107584696653632 2202 47 0.8387477667774945 2202 48 0.7848363589700398 2202 49 1.3523638114561152 2202 50 1.0659949998964393 2202 51 1.324733518272268 1102 1 0.0775442796745125 1102 2 0.09381017858269303 1102 3 0.3594115984770667 1102 4 -0.4678435313527416 1102 5 0.20575475451889247 1102 6 0.10526847929380523 1102 7 -0.074813596363839 1102 8 0.3563926981594321 1102 9 0.10034291846949372 1102 10 0.6053862549777378 1102 11 0.6524106053211653 1102 12 0.16793213093112058 1102 13 -0.5533096797952197 1102 14 -0.11118575283578025 1102 15 -0.2197850230802061 1102 16 -0.4144937031687732 1102 17 -0.01565870454261642 1102 18 0.2254058450128369 1102 19 0.2351240596764228 1102 20 0.5304618951482354 1102 21 -0.2066203878152848 1102 22 0.20752966293851927 1102 23 0.33847922933583874 1102 24 0.5605160937704596 1102 25 0.3104939717211934 1102 26 0.4678121927184762 1102 27 0.07976709726576559 1102 28 0.15597426847360651 1102 29 0.32617503654090996 1102 30 0.5126317042502961 1102 31 0.025275960496188077 1102 32 0.6645625502591486 1102 33 0.5237515315005155 1102 34 0.29055377513604697 1102 35 -0.3823479349875214 1102 36 0.11438484059583232 1102 37 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0.48093681134566424 2203 23 0.36567425653086616 2203 24 -0.37149633824197537 2203 25 -0.46611830489108286 2203 26 -0.03529581038952756 2203 27 0.11822863572730569 2203 28 -1.1546317921324558 2203 29 -0.5119174265515554 2203 30 -0.34430302768443255 2203 31 -0.41606048084025105 2203 32 0.14746853316513153 2203 33 -0.9749858422368618 2203 34 0.001023860606132132 2203 35 -1.054259356898946 2203 36 -0.5577208315098403 2203 37 -0.43185964739230087 2203 38 -0.37141046232260067 2203 39 -0.6406456208122862 2203 40 -0.642253094892645 2203 41 -0.7818694145484244 2203 42 -0.6326070918649975 2203 43 -0.9476237956271163 2203 44 -0.6432817931831707 2203 45 -0.2749646913279662 2203 46 -0.03347229956540332 2203 47 0.00220930523903462 2203 48 0.015605589739266179 2203 49 -0.24379003469773153 2203 50 -0.3186203847189475 2203 51 -0.8964203278815788 1103 1 0.6078473099775437 1103 2 0.021419606192125684 1103 3 -0.2011944621289956 1103 4 0.49680293329372205 1103 5 -0.09980080103665756 1103 6 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>>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.2652955142524 20.39167755440356 14.425197735350725 28.91782919599331 2 13.00910061232515 22.60547160271895 43.68013032975676 20.705297455199144 3 18.272336600848245 24.559728112799483 33.084290649664354 24.08364463668792 4 12.777013173047216 22.20318512484903 37.31988877341797 27.699912928685784 5 13.296632306266327 36.13319102322838 38.38581018453501 12.184366485970283 6 27.692891048507146 42.034379125354604 17.96337387298823 12.309355953150016 7 24.72825323708676 32.08858804033368 22.673651096817686 20.509507625761874 8 20.635901468977334 42.56382889082381 21.25804005280454 15.54222958739432 9 22.590792910709773 18.74139819678117 21.53610650787855 37.13170238463051 10 14.181389208774542 32.86661236412662 33.939555655422296 19.012442771676543 11 31.13080358396764 24.148245934331378 27.751874842007695 16.969075639693283 12 20.07976855882931 26.150886161278542 31.77822093643794 21.991124343454203 13 25.68042018930989 22.76914866724714 30.400528045389436 21.149903098053535 14 18.207735303204785 24.044322107687556 28.344521529084627 29.40342106002303 15 20.53197764233351 32.14757183383423 23.76344690054209 23.55700362329017 16 22.28744768699267 30.310647979102885 28.486363508693085 18.91554082521136 17 19.01946465185518 33.39325337752437 28.935763840125833 18.651518130494622 18 22.022948471272276 29.545103436652948 30.099855343173743 18.33209274890103 19 20.21599303429486 28.809369996910373 28.069263826082068 22.905373142712694 20 19.956183467685307 28.906271943375554 28.535516669943544 22.60202791899559 21 19.461843103109288 28.94138134426874 27.795410499115242 23.801365053506725 22 19.790467095469484 32.987388703199166 27.831924276044152 19.390219925287195 23 19.4829087436452 29.03968766676965 28.154930764261437 23.322472825323707 24 23.476954189253714 28.657697385051822 28.72229868269528 19.143049742999185 25 20.041850405864675 31.859674746510123 28.74336432323119 19.35511052439401 26 22.39137151363649 29.496109878381034 28.725107434766734 19.38741117321574 27 19.547510041288653 32.36244136730051 28.354352161334717 19.735696430076118 28 19.2568042018931 29.36128977895121 28.156335140297166 23.225570878858523 29 19.569980057860292 28.587478583265458 31.413083167148837 20.429458191725416 30 19.71518454019437 28.672546486152463 31.554407055783386 20.057861917869783 31 23.68058871443418 27.330562031289496 28.14931326011853 20.839535994157796 32 19.08968345364155 28.49057663680027 28.336095272870267 24.08364463668792 33 19.683734516754207 28.352947785298994 32.12791056933405 19.835407128612758 34 23.39971350728871 27.459764626576412 29.52138864702413 19.619133219110747 35 22.762126787068507 28.094542594725162 28.86835379041092 20.27497682779541 36 19.287700474679102 30.640676347498808 29.67587001095413 20.395753166867962 37 19.13041035867764 29.758728197062045 28.813583125017555 22.29727831924276 38 19.11355784624891 26.580625228211108 32.338566974693144 21.96724995084684 39 19.494143751931016 24.843412072016402 30.781113951071536 24.881330224981042 40 20.082577310900767 24.66646069151476 33.92832064713648 21.322641350447995 41 19.732887678004662 24.287279161868383 31.27966744375474 24.700165716372215 42 22.040277504704658 25.17624919248378 31.611100188186388 21.172373114625174 43 17.964778249023958 24.98665842766059 34.57995112771396 22.468612195601494 44 17.07019071426565 28.114203859225345 31.764177176080665 23.051428250428334 45 17.190967053338202 24.693143836193578 35.21753784793416 22.898351262534057 46 19.932309075077942 24.17352470297447 32.18127685869168 23.712889363255904 47 17.292082127910568 24.600455017835575 32.07313990394068 26.034322950313175 48 15.674240934752689 25.653737044631068 31.74170715950903 26.930314861107206 49 19.65143386793248 24.915035249838496 31.838609105974214 23.59492177625481 50 15.122321152711852 25.934612251776535 34.45074853242704 24.49231806308457 51 14.580846312655366 25.79385700040729 31.061893459545242 28.563403227392104 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 24.0 1 13.5 2 3.0 3 3.0 4 3.0 5 3.0 6 3.0 7 4.0 8 5.0 9 4.0 10 3.0 11 9.5 12 16.0 13 22.0 14 28.0 15 38.0 16 48.0 17 37.0 18 26.0 19 28.5 20 31.0 21 35.0 22 39.0 23 37.0 24 35.0 25 43.0 26 94.0 27 137.0 28 310.5 29 484.0 30 911.0 31 1338.0 32 2271.0 33 3204.0 34 4623.0 35 6042.0 36 7615.5 37 9189.0 38 10188.5 39 11188.0 40 10978.0 41 10768.0 42 9367.5 43 7967.0 44 6640.5 45 5314.0 46 4245.0 47 3176.0 48 2594.5 49 2013.0 50 1920.0 51 1827.0 52 2717.0 53 3607.0 54 2355.0 55 1103.0 56 989.0 57 875.0 58 808.0 59 741.0 60 672.5 61 604.0 62 520.5 63 437.0 64 384.0 65 331.0 66 268.5 67 206.0 68 170.0 69 134.0 70 105.0 71 76.0 72 68.5 73 61.0 74 44.5 75 25.0 76 22.0 77 16.5 78 11.0 79 7.0 80 3.0 81 3.0 82 3.0 83 2.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03510940089318316 2 0.002808752071454653 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0042131281071819794 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.002808752071454653 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0042131281071819794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 71206.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.12737686617551 #Duplication Level Percentage of deduplicated Percentage of total 1 94.94409089113877 84.62117770070947 2 3.9421868448620043 7.027135451977904 3 0.6085364735361629 1.6271177884101307 4 0.19026657310178913 0.6783184226351556 5 0.12416230034417716 0.5533130067673375 6 0.06206549876742142 0.3319041059418672 7 0.03208792305909118 0.20019386879383239 8 0.014799712367346039 0.10552476333403592 9 0.0069257674081684515 0.055554793368777465 >10 0.06708686622507272 0.9938229113636479 >50 0.004675410534325597 0.3138896994090117 >100 0.0015578693283801216 0.23465448338855652 >500 0.0 0.0 >1k 0.0015578693273318868 3.257393003900249 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 2346 3.294666179816308 No Hit TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT 169 0.23733955003791815 Illumina PCR Primer Index 8 (95% over 21bp) CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 98 0.137628851501278 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT 73 0.10251945060809484 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0014043760357273264 4 0.0 0.0 0.002808752071454653 5 0.0 0.0 0.0042131281071819794 6 0.0 0.0 0.0042131281071819794 7 0.0 0.0 0.0042131281071819794 8 0.0 0.0 0.0042131281071819794 9 0.0 0.0 0.009830632250091285 10 0.0 0.0 0.014043760357273264 11 0.0 0.0 0.01544813639300059 12 0.0 0.0 0.018256888464455244 13 0.0 0.0 0.018256888464455244 14 0.0 0.0 0.018256888464455244 15 0.0 0.0 0.025278768643091875 16 0.0 0.0 0.025278768643091875 17 0.0 0.0 0.03089627278600118 18 0.0 0.0 0.043535657107547115 19 0.0 0.0 0.05055753728618375 20 0.0 0.0 0.05898379350054771 21 0.0 0.0 0.0744319298935483 22 0.0 0.0 0.09971069853664018 23 0.0 0.0 0.16431199618009718 24 0.0 0.0 0.23172204589500886 25 0.0 0.0 0.25419206246664605 26 0.0 0.0 0.36513776928910485 27 0.0 0.0 0.39182091396792407 28 0.0 0.0 0.42973906693256186 29 0.0 0.0 0.5168103811476561 30 0.0 0.0 0.6951661376850266 31 0.0 0.0 1.4001629076201443 32 0.0 0.0 2.2441929050922673 33 0.0 0.0 3.05170912563548 34 0.0 0.0 4.046007358930427 35 0.0 0.0 4.985534926832009 36 0.0 0.0 6.1792545572002355 37 0.0 0.0 7.856079543858663 38 0.0 0.0 9.832036626127012 39 0.0 0.0 11.977923208718366 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGTAT 295 0.0 40.423725 39 AATCTCG 285 0.0 40.263157 36 CGTATGC 300 0.0 39.75 41 TATGCCG 300 0.0 39.75 43 TCTCGTA 300 0.0 39.75 38 TCGTATG 300 0.0 39.75 40 GGAATCT 295 0.0 39.661015 34 TGGAATC 295 0.0 39.661015 33 CGAGACT 295 0.0 39.661015 23 TGCTGGA 290 0.0 39.568966 30 ATCTCGT 305 0.0 39.098362 37 GCCCACG 305 0.0 39.098362 18 CACGAGA 305 0.0 39.098362 21 CCCACGA 305 0.0 39.098362 19 GTATGCC 305 0.0 39.098362 42 ACGAGAC 305 0.0 39.098362 22 CCACGAG 305 0.0 39.098362 20 TGCCGTC 305 0.0 39.098362 45 GCTGGAA 295 0.0 38.898304 31 GAATCTC 295 0.0 38.898304 35 >>END_MODULE