FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_77.34100000006ce5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_77.34100000006ce5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71206
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC23463.294666179816308No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT1690.23733955003791815Illumina PCR Primer Index 8 (95% over 21bp)
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA980.137628851501278No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT730.10251945060809484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2950.040.42372539
AATCTCG2850.040.26315736
CGTATGC3000.039.7541
TATGCCG3000.039.7543
TCTCGTA3000.039.7538
TCGTATG3000.039.7540
GGAATCT2950.039.66101534
TGGAATC2950.039.66101533
CGAGACT2950.039.66101523
TGCTGGA2900.039.56896630
ATCTCGT3050.039.09836237
GCCCACG3050.039.09836218
CACGAGA3050.039.09836221
CCCACGA3050.039.09836219
GTATGCC3050.039.09836242
ACGAGAC3050.039.09836222
CCACGAG3050.039.09836220
TGCCGTC3050.039.09836245
GCTGGAA2950.038.89830431
GAATCTC2950.038.89830435
Produced by FastQC (version 0.11.2)