FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_76.34100000006cd8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_76.34100000006cd8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72207
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC8731.2090240558394616No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.217430443031839No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA970.13433600620438463No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT960.13295109892392704No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA940.1301812843630119No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT920.12741146980209675Illumina PCR Primer Index 8 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.11633221155843616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACT1050.038.57083523
TGCCGTC1050.038.57083545
CCGAGCC1150.037.1733414
TATGCCG1100.036.81761643
CCCACGA1100.036.81761619
TCGTATG1100.036.81761640
CGAGCCC1100.036.81761615
ACGAGAC1100.036.81761622
CCACGAG1100.036.81761620
CTCGTAT1050.036.4280139
GACTTGC1050.036.4280126
CACGAGA1150.035.21684621
ATGCCGT1150.035.21684644
GAGACTT1100.034.77219424
TCTCGTA1100.034.77219438
GGAATCT1050.034.28518734
AATCTCG1050.034.28518736
ATCTCCG1200.033.7494810
CGTATGC1200.033.7494841
AGCCCAC1200.033.7494817