##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_75.34100000006ccb.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50011 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.282557837275796 38.0 38.0 38.0 32.0 38.0 2 36.33908540121173 38.0 38.0 38.0 32.0 38.0 3 36.27309991801803 38.0 38.0 38.0 32.0 38.0 4 36.393673391853795 38.0 38.0 38.0 32.0 38.0 5 36.40837015856511 38.0 38.0 38.0 32.0 38.0 6 38.274639579292554 40.0 38.0 40.0 38.0 40.0 7 38.14818739877227 40.0 38.0 40.0 38.0 40.0 8 38.1469276759113 40.0 38.0 40.0 38.0 40.0 9 38.205874707564334 40.0 38.0 40.0 38.0 40.0 10 38.11797404570994 40.0 38.0 40.0 38.0 40.0 11 38.12325288436544 40.0 38.0 40.0 38.0 40.0 12 38.045290036192036 40.0 38.0 40.0 38.0 40.0 13 38.096598748275376 40.0 38.0 40.0 38.0 40.0 14 38.03151306712523 40.0 38.0 40.0 38.0 40.0 15 38.030233348663295 40.0 38.0 40.0 32.0 40.0 16 38.017376177241005 40.0 38.0 40.0 32.0 40.0 17 38.02583431645038 40.0 38.0 40.0 32.0 40.0 18 38.05280838215592 40.0 38.0 40.0 38.0 40.0 19 38.06900481893983 40.0 38.0 40.0 38.0 40.0 20 38.06022675011498 40.0 38.0 40.0 38.0 40.0 21 38.04670972386075 40.0 38.0 40.0 38.0 40.0 22 38.06084661374498 40.0 38.0 40.0 38.0 40.0 23 38.04962908160205 40.0 38.0 40.0 32.0 40.0 24 38.00823818759873 40.0 38.0 40.0 32.0 40.0 25 38.00767831077163 40.0 38.0 40.0 32.0 40.0 26 37.96168842854572 40.0 38.0 40.0 32.0 40.0 27 37.93411449481114 40.0 38.0 40.0 32.0 40.0 28 37.92763592009758 40.0 38.0 40.0 32.0 40.0 29 37.92789586291016 40.0 38.0 40.0 32.0 40.0 30 37.90542080742237 40.0 38.0 40.0 32.0 40.0 31 37.89206374597588 40.0 38.0 40.0 32.0 40.0 32 37.87234808342165 40.0 38.0 40.0 32.0 40.0 33 37.8138409549899 40.0 38.0 40.0 32.0 40.0 34 37.873087920657454 40.0 38.0 40.0 32.0 40.0 35 37.88694487212813 40.0 38.0 40.0 32.0 40.0 36 37.807882265901505 40.0 38.0 40.0 32.0 40.0 37 37.825138469536704 40.0 38.0 40.0 32.0 40.0 38 37.84601387694707 40.0 38.0 40.0 32.0 40.0 39 37.751434684369436 40.0 38.0 40.0 32.0 40.0 40 37.75491391893783 40.0 38.0 40.0 32.0 40.0 41 37.80520285537182 40.0 38.0 40.0 32.0 40.0 42 37.790146167843076 40.0 38.0 40.0 32.0 40.0 43 37.76245226050269 40.0 38.0 40.0 32.0 40.0 44 37.71342304692968 40.0 38.0 40.0 32.0 40.0 45 37.67931055167863 40.0 38.0 40.0 32.0 40.0 46 37.612185319229766 40.0 38.0 40.0 32.0 40.0 47 37.62274299654076 40.0 38.0 40.0 32.0 40.0 48 37.54496010877607 40.0 38.0 40.0 32.0 40.0 49 37.52584431425087 40.0 38.0 40.0 32.0 40.0 50 37.53942132730799 40.0 38.0 40.0 32.0 40.0 51 37.070504489012414 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.6470931790844787 2201 2 0.16746353236270295 2201 3 -0.0038487033915330926 2201 4 -0.5643132156900421 2201 5 0.07125256810939362 2201 6 0.3623790242035696 2201 7 0.22596315363012565 2201 8 -0.6608465155775036 2201 9 -0.4929369146102971 2201 10 -0.5467053843638752 2201 11 0.081162681434229 2201 12 0.3556111484666573 2201 13 0.08434589471994514 2201 14 0.22759384850524356 2201 15 -0.8523058146011024 2201 16 -0.12899764550562764 2201 17 0.18991835250844957 2201 18 0.7805607342662526 2201 19 0.5057975216420232 2201 20 0.2885552068008721 2201 21 0.8582454136857294 2201 22 0.4965954990084356 2201 23 0.2971375625360295 2201 24 -0.23730122701420697 2201 25 0.7710844989971193 2201 26 0.23003137729335066 2201 27 0.01778262071184855 2201 28 -0.26038375405698844 2201 29 -0.07379792518008088 2201 30 0.34551339045303564 2201 31 0.040488483347097315 2201 32 0.10279745892126613 2201 33 0.48820590098502237 2201 34 0.7947285093561263 2201 35 0.47451786878096414 2201 36 0.48866611913746283 2201 37 0.4211152757544454 2201 38 0.0870242491703479 2201 39 0.26276041852649 2201 40 -0.31263684711488793 2201 41 0.003338285494699278 2201 42 0.5237667686095335 2201 43 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1212 19 -0.6399686416025077 1212 20 -0.23443817517774335 1212 21 -0.3805124346088533 1212 22 -0.27754330974772046 1212 23 -0.4744558533811585 1212 24 0.0846061206864519 1212 25 -0.5698284485677902 1212 26 -0.01721111379777085 1212 27 0.5460364812243199 1212 28 -0.33996970348851363 1212 29 -0.03341102546855268 1212 30 -0.04647710674702665 1212 31 -0.5656543980375943 1212 32 0.07276182342152282 1212 33 0.2526722169531226 1212 34 -0.6883870514787631 1212 35 0.3051643985620558 1212 36 0.4058560054432476 1212 37 -0.5191528389579219 1212 38 0.13725333452585886 1212 39 -0.30350464171447555 1212 40 -0.15906481249754734 1212 41 -0.0665751711487772 1212 42 -0.9747908421150555 1212 43 -0.7235863122364918 1212 44 -0.9432497326576481 1212 45 -0.23336101115192776 1212 46 0.4153136426679964 1212 47 0.1561685719249084 1212 48 0.21955637579258536 1212 49 -0.11323210091030944 1212 50 -1.4166586890034765 1212 51 -0.061010037931659156 1213 1 -0.6650189153200756 1213 2 -0.6664033050252627 1213 3 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39 0.13776041852649 1213 40 0.05260002868536162 1213 41 -0.7908358502287314 1213 42 0.25344026412809484 1213 43 -0.3526936506811751 1213 44 -0.6048050448153717 1213 45 -0.5761868600018687 1213 46 -0.36466992797822684 1213 47 0.06895608561603694 1213 48 -1.209249757832822 1213 49 0.10418301409517028 1213 50 -0.19034434070117356 1213 51 -0.4132827652043858 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 2.0 18 1.0 19 0.0 20 6.0 21 3.0 22 12.0 23 42.0 24 70.0 25 143.0 26 224.0 27 308.0 28 408.0 29 557.0 30 693.0 31 846.0 32 1091.0 33 1280.0 34 1529.0 35 2061.0 36 2790.0 37 4232.0 38 8156.0 39 25556.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.20640540919002 20.724559403068675 13.598991778191202 27.470043409550097 2 14.413117376524696 21.929614077184564 41.43571285742851 22.221555688862228 3 18.81586051068765 24.314650776829097 30.795225050488895 26.07426366199436 4 13.357061446481774 19.537701705624762 36.51796604746956 30.587270800423905 5 14.452820379516506 36.002079542500645 35.76413189098398 13.78096818699886 6 30.34732358881046 38.559516906280614 17.866069464717764 13.227090040191158 7 26.5401611645438 32.3908740077183 21.60124772550039 19.467717102237508 8 23.158905040891003 38.85945092079742 20.913399052208515 17.068244986103057 9 23.87874667573134 18.5199256163644 21.805202855371817 35.796124852532444 10 15.366619343744375 30.521285317230205 32.848773269880624 21.263322069144788 11 31.72365526894621 24.263147370525896 26.506698660267947 17.506498700259947 12 22.465057687308793 25.466397392573636 30.389314350842817 21.67923056927476 13 26.56015676551159 23.096918677890866 28.261782407870268 22.08114214872728 14 19.813640998980226 23.916738317570136 27.200015996480776 29.06960468696887 15 21.46527763891944 32.06694527204015 23.712783187698705 22.754993901341706 16 22.347530493901218 30.287942411517697 27.20455908818236 20.15996800639872 17 20.61146547759493 31.243126512167326 28.169802643418446 19.9756053668193 18 21.673231888984425 28.929635480194353 29.929415528583714 19.467717102237508 19 21.599248165403612 28.2777788886445 27.987842674611585 22.135130271340305 20 21.299314150886804 28.78166803303273 28.31976965067685 21.599248165403612 21 20.643458439143387 28.473735778128813 28.01383695586971 22.86896882685809 22 21.3093119513707 30.63925936294015 27.629921417288195 20.42150726840095 23 20.93939333346664 28.98762272300094 27.865869508708087 22.207114434824337 24 22.69700665853512 28.22579032612825 28.931635040291138 20.145567975045488 25 21.287316790306132 29.615484593389453 28.369758653096316 20.727439963208095 26 21.95916898282378 28.597708504129095 28.96762712203315 20.475495391013975 27 20.82941752814381 30.265341624842534 28.197796484773352 20.707444362240306 28 20.789426326208236 28.577712903161306 28.175801323708782 22.457059446921676 29 21.399292155725743 27.93785367219212 29.68346963667993 20.97938453540221 30 20.981803639272144 27.86042791441712 29.838032393521296 21.31973605278944 31 23.112915158665093 27.963847953450237 28.21379296554758 20.709443922337087 32 20.90140169162784 28.345763931934975 28.327767891063964 22.42506648537322 33 20.75143468436944 28.149807042450657 30.377316990262145 20.72144128291776 34 22.86896882685809 28.281778008838053 28.72368079022615 20.125572374077702 35 22.71500269940613 28.505728739677274 28.20979384535402 20.569474715562578 36 20.557477354981906 29.791445881905982 28.545719941612845 21.10535682149927 37 20.467497150626862 28.961628441742814 28.281778008838053 22.289096398792267 38 20.75143468436944 27.925856311611447 29.907420367519144 21.41528863649997 39 21.2153326268221 26.800103977125033 28.701685629161585 23.282877766891282 40 21.845194057307392 26.566155445801925 30.425306432584833 21.163344064305853 41 21.41528863649997 25.776329207574335 29.307552338485532 23.500829817440163 42 22.591029973405853 26.586151046769714 29.477514946711725 21.345304033112715 43 20.233548619303754 26.682129931415087 31.007178420747433 22.077143028533722 44 19.567695107076442 28.10581672032153 29.771450280938193 22.555037891663833 45 19.937613724980505 26.496170842414667 30.84521405290836 22.721001379696467 46 21.963168103017335 25.906300613864953 29.571494271260324 22.55903701185739 47 20.571474275659355 26.5401611645438 28.769670672452058 24.118693887344783 48 19.181780008398153 26.904081102157523 29.49751054767951 24.416628341764813 49 21.403291275919297 26.266221431285118 29.49751054767951 22.832976745116074 50 19.143788366559356 27.31399092199716 30.771230329327548 22.770990382115937 51 18.892221555688863 27.13257348530294 29.0501899620076 24.9250149970006 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 51.0 1 27.5 2 4.0 3 4.5 4 5.0 5 7.5 6 10.0 7 7.5 8 5.0 9 10.0 10 15.0 11 21.5 12 28.0 13 54.5 14 81.0 15 102.0 16 123.0 17 124.5 18 126.0 19 125.5 20 125.0 21 113.0 22 101.0 23 91.5 24 82.0 25 124.0 26 265.5 27 365.0 28 635.5 29 906.0 30 1508.0 31 2110.0 32 2828.0 33 3546.0 34 3998.5 35 4451.0 36 4567.5 37 4684.0 38 4499.5 39 4315.0 40 3945.5 41 3576.0 42 3412.0 43 3248.0 44 3122.5 45 2997.0 46 2867.5 47 2738.0 48 2690.5 49 2643.0 50 2608.0 51 2573.0 52 2828.0 53 3083.0 54 2533.0 55 1983.0 56 1794.0 57 1605.0 58 1415.5 59 1226.0 60 1122.0 61 1018.0 62 874.0 63 730.0 64 603.5 65 477.0 66 401.0 67 325.0 68 254.5 69 184.0 70 158.0 71 132.0 72 110.5 73 89.0 74 69.0 75 34.0 76 19.0 77 13.5 78 8.0 79 7.0 80 6.0 81 3.5 82 1.0 83 1.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04399032212913159 2 0.001999560096778709 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.001999560096778709 12 0.0 13 0.0 14 0.0 15 0.0 16 0.001999560096778709 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.001999560096778709 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.001999560096778709 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 50011.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.44514582748478 #Duplication Level Percentage of deduplicated Percentage of total 1 95.02091150166358 86.8920110893015 2 4.020598209785065 7.353283792150391 3 0.5986774446190504 1.6423843868044499 4 0.12705283711613904 0.4647346087152402 5 0.07330376828032989 0.335163689004946 6 0.04284052492589166 0.23505348295044956 7 0.024317792339563025 0.1556620846685683 8 0.014275055093672679 0.10443075957890219 9 0.024455973377149495 0.2012742046643869 >10 0.047002585981452766 0.8412502208308722 >50 0.004376292142849754 0.21810344354393324 >100 0.0 0.0 >500 0.002188014675264426 1.556648237786366 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 778 1.5556577552938353 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 58 0.11597448561316512 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51 0.10197756493571415 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.001999560096778709 4 0.0 0.0 0.001999560096778709 5 0.0 0.0 0.001999560096778709 6 0.0 0.0 0.001999560096778709 7 0.0 0.0 0.001999560096778709 8 0.0 0.0 0.001999560096778709 9 0.0 0.0 0.001999560096778709 10 0.0 0.0 0.001999560096778709 11 0.0 0.0 0.001999560096778709 12 0.0 0.0 0.001999560096778709 13 0.0 0.0 0.001999560096778709 14 0.0 0.0 0.001999560096778709 15 0.0 0.0 0.001999560096778709 16 0.0 0.0 0.003999120193557418 17 0.0 0.0 0.003999120193557418 18 0.0 0.0 0.003999120193557418 19 0.0 0.0 0.005998680290336126 20 0.0 0.0 0.007998240387114835 21 0.0 0.0 0.007998240387114835 22 0.0 0.0 0.009997800483893543 23 0.0 0.0 0.011997360580672252 24 0.0 0.0 0.013996920677450961 25 0.0 0.0 0.01599648077422967 26 0.0 0.0 0.023994721161344504 27 0.0 0.0 0.03199296154845934 28 0.0 0.0 0.049989002419467715 29 0.0 0.0 0.07998240387114834 30 0.0 0.0 0.15196656735518185 31 0.0 0.0 0.3719181780008398 32 0.0 0.0 0.6418587910659654 33 0.0 0.0 0.9577892863570014 34 0.0 0.0 1.3257083441642838 35 0.0 0.0 1.74961508468137 36 0.0 0.0 2.265501589650277 37 0.0 0.0 2.9473515826518164 38 0.0 0.0 3.8051628641698825 39 0.0 0.0 4.684969306752515 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATGC 115 0.0 41.085133 41 CTCGTAT 115 0.0 41.085133 39 CACGAGA 115 0.0 41.085133 21 GACTTGC 115 0.0 41.085133 26 TCTCGTA 115 0.0 41.085133 38 TCGTATG 115 0.0 41.085133 40 ACGAGAC 115 0.0 41.085133 22 CCACGAG 115 0.0 41.085133 20 TGCCGTC 115 0.0 41.085133 45 AGACTTG 120 0.0 39.373253 25 ATCTCGT 120 0.0 39.373253 37 TATGCCG 120 0.0 39.373253 43 GTATGCC 120 0.0 39.373253 42 CCGAGCC 120 0.0 39.373253 14 CGAGACT 120 0.0 39.373253 23 ATGCCGT 120 0.0 39.373253 44 GGAATCT 130 0.0 38.07523 34 GAATCTC 125 0.0 37.79832 35 CCCACGA 125 0.0 37.79832 19 GAGACTT 125 0.0 37.79832 24 >>END_MODULE