FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_75.34100000006ccb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_75.34100000006ccb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50011
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC7781.5556577552938353No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA580.11597448561316512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.10197756493571415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1150.041.08513341
CTCGTAT1150.041.08513339
CACGAGA1150.041.08513321
GACTTGC1150.041.08513326
TCTCGTA1150.041.08513338
TCGTATG1150.041.08513340
ACGAGAC1150.041.08513322
CCACGAG1150.041.08513320
TGCCGTC1150.041.08513345
AGACTTG1200.039.37325325
ATCTCGT1200.039.37325337
TATGCCG1200.039.37325343
GTATGCC1200.039.37325342
CCGAGCC1200.039.37325314
CGAGACT1200.039.37325323
ATGCCGT1200.039.37325344
GGAATCT1300.038.0752334
GAATCTC1250.037.7983235
CCCACGA1250.037.7983219
GAGACTT1250.037.7983224
Produced by FastQC (version 0.11.2)