FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_74.34100000006cbe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_74.34100000006cbe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC71552.84552845528455No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT735.395417590539542Illumina PCR Primer Index 8 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG110.8130081300813009No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTTC100.7390983000739099Illumina PCR Primer Index 8 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT80.5912786400591279No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTT60.4434589800443459Illumina PCR Primer Index 8 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC50.36954915003695493TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC40.29563932002956395No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC40.29563932002956395No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC30.22172949002217296No Hit
AGAGACAGCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGT20.14781966001478197No Hit
GTCCTACAGCTGCTTCGCAGGCTGCGAAAACTAAGATGGTGATGGGGATTG20.14781966001478197No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC20.14781966001478197No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATTCGTATGCCGTCT20.14781966001478197No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACT20.14781966001478197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC850.039.7058831
AGCCCAC900.037.517
GTATGCC900.037.542
AATCTCG900.037.536
ATCTCGT900.037.537
ATCTCCG900.037.510
GCCCACG900.037.518
CCACGAG900.037.520
TATGCCG900.037.543
CATCTCC900.037.59
GAGCCCA900.037.516
CACGAGA900.037.521
CCCACGA900.037.519
ACATCTC900.037.58
TCTCGTA900.037.538
ACGAGAC900.037.522
ATGCCGT900.037.544
CGTATGC900.037.541
CGAGCCC900.037.515
CCGAGCC900.037.514