##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_73.34100000006ca2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11908 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.32927443735304 38.0 38.0 38.0 32.0 38.0 2 36.319281155525694 38.0 38.0 38.0 32.0 38.0 3 36.28468256634196 38.0 38.0 38.0 32.0 38.0 4 36.451965065502186 38.0 38.0 38.0 32.0 38.0 5 36.4669969768223 38.0 38.0 38.0 32.0 38.0 6 38.356399059455825 40.0 38.0 40.0 38.0 40.0 7 38.26839099764864 40.0 38.0 40.0 38.0 40.0 8 38.306264696002685 40.0 38.0 40.0 38.0 40.0 9 38.2739334900907 40.0 38.0 40.0 38.0 40.0 10 38.196842458851194 40.0 38.0 40.0 38.0 40.0 11 38.185925428283504 40.0 38.0 40.0 38.0 40.0 12 38.10396372186765 40.0 38.0 40.0 38.0 40.0 13 38.2215317433658 40.0 38.0 40.0 38.0 40.0 14 38.10765871682902 40.0 38.0 40.0 38.0 40.0 15 38.0718844474303 40.0 38.0 40.0 32.0 40.0 16 38.07322808196171 40.0 38.0 40.0 32.0 40.0 17 38.104635539133355 40.0 38.0 40.0 38.0 40.0 18 38.153930131004365 40.0 38.0 40.0 38.0 40.0 19 38.14981525025193 40.0 38.0 40.0 38.0 40.0 20 38.09170305676856 40.0 38.0 40.0 38.0 40.0 21 38.11471279811891 40.0 38.0 40.0 38.0 40.0 22 38.133523681558614 40.0 38.0 40.0 38.0 40.0 23 38.119583473295265 40.0 38.0 40.0 38.0 40.0 24 38.07860262008734 40.0 38.0 40.0 32.0 40.0 25 38.043668122270745 40.0 38.0 40.0 32.0 40.0 26 38.122270742358076 40.0 38.0 40.0 38.0 40.0 27 38.06768558951965 40.0 38.0 40.0 32.0 40.0 28 38.00982532751092 40.0 38.0 40.0 32.0 40.0 29 38.00285522337924 40.0 38.0 40.0 32.0 40.0 30 37.98807524353376 40.0 38.0 40.0 32.0 40.0 31 38.03535438360766 40.0 38.0 40.0 32.0 40.0 32 37.98563990594558 40.0 38.0 40.0 32.0 40.0 33 37.914679207255624 40.0 38.0 40.0 32.0 40.0 34 37.908884783338934 40.0 38.0 40.0 32.0 40.0 35 37.86479677527713 40.0 38.0 40.0 32.0 40.0 36 37.78930131004367 40.0 38.0 40.0 32.0 40.0 37 37.85614712798119 40.0 38.0 40.0 32.0 40.0 38 37.8218844474303 40.0 38.0 40.0 32.0 40.0 39 37.78644608666443 40.0 38.0 40.0 32.0 40.0 40 37.69675848169298 40.0 38.0 40.0 32.0 40.0 41 37.70213301981861 40.0 38.0 40.0 32.0 40.0 42 37.67584816929795 40.0 38.0 40.0 32.0 40.0 43 37.61706415854888 40.0 38.0 40.0 32.0 40.0 44 37.5608834397044 40.0 38.0 40.0 32.0 40.0 45 37.52166610681895 40.0 38.0 40.0 32.0 40.0 46 37.37302653678199 40.0 38.0 40.0 32.0 40.0 47 37.326083305340944 40.0 38.0 40.0 32.0 40.0 48 37.2648639570037 40.0 38.0 40.0 32.0 40.0 49 37.16090023513604 40.0 38.0 40.0 32.0 40.0 50 37.1793752099429 40.0 38.0 40.0 32.0 40.0 51 36.67803157541149 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.36680242678276187 2201 2 0.6260907076483804 2201 3 -1.1024577503037705 2201 4 -0.05796051720327 2201 5 0.36710625791189955 2201 6 0.8033934164676708 2201 7 0.9823330413162594 2201 8 0.3615855159771044 2201 9 0.6842186077199415 2201 10 0.587923421418509 2201 11 0.9191623484304472 2201 12 1.4593062214917865 2201 13 1.2281513212797606 2201 14 1.2520971543478296 2201 15 1.2138002908716814 2201 16 0.9596793625548727 2201 17 0.9074006939970758 2201 18 1.3718301319318442 2201 19 0.5783723285629279 2201 20 1.1815191428911405 2201 21 0.9510036470023948 2201 22 0.8395296011006792 2201 23 1.0223528650894096 2201 24 0.8428462734026922 2201 25 0.8656930612655671 2201 26 0.6539220473582787 2201 27 1.0506274691720918 2201 28 0.7384363087459391 2201 29 0.9519923875282146 2201 30 0.4812432888153424 2201 31 0.8684369189219154 2201 32 -0.3113405400201543 2201 33 1.0380973797697735 2201 34 0.9754007574844081 2201 35 0.903832366838067 2201 36 1.1384448704363521 2201 37 1.7127264482131181 2201 38 1.6138364412132873 2201 39 1.6523936639190495 2201 40 1.2960361754394256 2201 41 1.594525848335138 2201 42 1.5562764538706517 2201 43 1.674054896400989 2201 44 1.7063527971148815 2201 45 0.6695109608651322 2201 46 0.19034713088435495 2201 47 -0.033537280325433017 2201 48 -0.11687194157813252 2201 49 -0.6588487076026723 2201 50 0.7555049231040059 2201 51 0.720083742581231 1101 1 0.36680242678276187 1101 2 1.257669655016798 1101 3 0.7922790918014968 1101 4 1.1525657985862026 1101 5 0.05131678422768715 1101 6 0.9086565743624107 1101 7 -0.12293011657848041 1101 8 0.7300065686086796 1101 9 0.947376502456784 1101 10 1.008976052997454 1101 11 0.7086360326409746 1101 12 0.3014114846496767 1101 13 1.1228881633850278 1101 14 0.30472873329519956 1101 15 1.0032739750822017 1101 16 1.2754688362390851 1101 17 1.0126638518918156 1101 18 0.7402511845634194 1101 19 1.3152144338260854 1101 20 1.0762559849964006 1101 21 1.2667931206866072 1101 22 1.0500559168901518 1101 23 0.811826549299937 1101 24 1.1586357470869046 1101 25 1.0762193770550397 1101 26 0.7591852052530115 1101 27 1.2611537849615644 1101 28 0.9489626245354117 1101 29 1.4783081770018995 1101 30 1.3233485519732398 1101 31 0.8684369189219154 1101 32 1.4255015652430103 1101 33 1.4591500113487257 1101 34 1.3964533890633533 1101 35 0.7985692089433272 1101 36 1.4542343441205574 1101 37 1.6074632903183783 1101 38 1.4033101254238147 1101 39 0.915551558655892 1101 40 0.45393091228152826 1101 41 0.7524205851772408 1101 42 -0.07530249349777307 1101 43 0.9372127911378314 1101 44 1.2853001655359293 1101 45 1.4063530661282897 1101 46 1.6113997624633072 1101 47 0.3875153512535121 1101 48 0.19891753210607988 1101 49 0.23588813450259494 1101 50 -0.7181792874223092 1101 51 -0.1746530995240363 2202 1 -0.6200396784803957 2202 2 0.44845912870101046 2202 3 -0.1353524871458731 2202 4 1.093355272270415 2202 5 -0.18223584735125797 2202 6 0.2704986796255682 2202 7 0.969175146579417 2202 8 0.13461183176657698 2202 9 -0.7532813922800585 2202 10 0.7458181582606116 2202 11 1.0112676115883446 2202 12 0.6303588530707316 2202 13 0.4419671107534455 2202 14 -0.6590870561784854 2202 15 -0.8421207617599009 2202 16 -0.42518905849775734 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0.9954778523500636 1209 25 0.6130614823181944 1209 26 1.0012904684109074 1209 27 0.7822064165405109 1209 28 0.7410678876933119 1209 29 1.2993608085808432 1209 30 0.5496643414469276 1209 31 0.8210684978692839 1209 32 0.9570805126114266 1209 33 -0.05927104128285521 1209 34 0.8227691785370368 1209 35 1.4669902615749066 1209 36 -4.103660392721544 1209 37 1.1337790797920633 1209 38 0.7401522306869737 1209 39 -0.07129054660726553 1209 40 1.2434045964920557 1209 41 1.120841637808823 1209 42 1.1878554012390694 1209 43 0.9503706858746739 1209 44 0.787931744483302 1209 45 0.9037214871809169 1209 46 1.4087681835159387 1209 47 0.6059364038850958 1209 48 0.27260174263239634 1209 49 -0.2799013391816203 1209 50 1.7160312388934784 1209 51 0.6437679531075489 1214 1 0.01884921040849008 1214 2 -0.09320753796565384 1214 3 -0.31590804270143025 1214 4 0.8016886056037507 1214 5 -1.0919580695734794 1214 6 -2.021167987041096 1214 7 -1.725269297865026 1214 8 -0.8167770571223087 1214 9 0.0731074966088272 1214 10 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1213 38 -1.1325750420403011 1213 39 -2.6849269102436324 1213 40 -1.4384135853261242 1213 41 0.029932546899729573 1213 42 0.41512812851179604 1213 43 -2.299629314125326 1213 44 -0.8438864373348807 1213 45 -0.2371876037281737 1213 46 -2.6685045437567894 1213 47 0.11957276752146129 1213 48 -2.2819437119130583 1213 49 -3.2617195209997973 1213 50 -0.6703323974701547 1213 51 -2.8516865923470007 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 1.0 22 2.0 23 13.0 24 24.0 25 29.0 26 44.0 27 68.0 28 97.0 29 112.0 30 150.0 31 227.0 32 267.0 33 320.0 34 427.0 35 476.0 36 679.0 37 973.0 38 1863.0 39 6135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.38723225535489 20.19319613607728 13.473330533389333 27.946241075178495 2 14.519650655021834 22.363117232112863 39.69600268726906 23.421229425596238 3 18.903258313738664 24.613705072220355 30.861605643265033 25.62143097077595 4 13.696674504534768 19.936177359758144 35.13604299630501 31.231105139402082 5 13.898219684245886 36.28653006382264 34.984884111521666 14.83036614040981 6 30.508901578770576 38.89821968424588 16.669465905273764 13.923412831709776 7 26.175680214981522 32.54114880752436 20.482028888142427 20.801142089351696 8 24.051058112193484 37.185085656701375 20.22169969768223 18.54215653342291 9 22.82499160228418 18.794088008061806 21.212630164595232 37.16829022505878 10 15.67013772253947 31.357070876721533 30.861605643265033 22.111185757473965 11 30.340947262344642 24.462546187437017 25.629828686597243 19.566677863621095 12 22.850184749748067 26.217668794088006 28.49344978165939 22.438696674504534 13 26.746724890829693 23.253275109170303 28.081961706415854 21.918038293584143 14 20.901914679207255 23.857910648303662 25.982532751091703 29.257641921397383 15 21.666106818945245 31.10513940208263 23.681558616056435 23.54719516291569 16 22.826908541194253 30.410682791635175 25.900730662635425 20.861678004535147 17 20.431642593214647 31.516627477326164 26.93987235471952 21.11185757473967 18 22.531071548538797 28.300302317769564 29.475982532751093 19.692643600940542 19 20.120927107826674 28.317097749412163 28.745381256298288 22.816593886462883 20 21.221027880416525 27.368155861605644 29.03930131004367 22.371514947934163 21 20.07893852872019 27.695666778636213 28.443063486731607 23.78233120591199 22 20.19650655021834 30.416526704736313 29.030903594222373 20.356063150822976 23 20.89351696338596 28.14914343298623 28.258313738663084 22.69902586496473 24 22.732616728249916 28.51024521330198 28.49344978165939 20.263688276788713 25 21.35539133355727 29.526368827678873 28.703392677191804 20.41484716157205 26 22.547866980181393 28.75377897211958 28.854551561975146 19.843802485723884 27 20.129324823647966 30.147799798454823 29.408800806180718 20.314074571716493 28 20.146120255290562 28.06516627477326 28.552233792408465 23.236479677527715 29 20.330870003359085 28.73698354047699 30.752435337588174 20.179711118575746 30 19.818609338259993 28.32549546523346 31.70977494121599 20.146120255290562 31 23.127309371850856 27.191803829358413 28.77057440376218 20.91031239502855 32 20.775948941887805 27.141417534430634 29.064494457507557 23.018139066174 33 19.96976822304333 27.250587840107492 31.936513268390996 20.84313066845818 34 22.732616728249916 27.62008733624454 29.064494457507557 20.582801477997982 35 22.94255962378233 27.54450789385287 28.91333557272422 20.599596909640578 36 19.961370507222036 30.282163251595566 28.98051729929459 20.775948941887805 37 20.0537453812563 29.232448773933488 29.37520994289553 21.33859590191468 38 19.659052737655358 26.822304333221364 31.860933825999325 21.657709103123953 39 19.818609338259993 26.797111185757473 29.837084313066846 23.54719516291569 40 20.84313066845818 26.486395700369496 31.961706415854884 20.708767215317433 41 20.7843466577091 26.394020826335236 29.576755122606652 23.244877393349007 42 23.160900235136044 26.620759153510242 29.333221363789047 20.885119247564663 43 20.557608330534094 26.15888478333893 31.88612697346322 21.397379912663755 44 19.675848169297954 29.64393684917702 29.18206247900571 21.498152502519314 45 19.95297279140074 26.847497480685256 32.230433322136385 20.96909640577763 46 21.892845146120255 27.091031239502854 29.16526704736312 21.85085656701377 47 20.52401746724891 26.2344642257306 28.745381256298288 24.496137050722204 48 19.222371514947934 25.957339603627815 29.249244205576087 25.57104467584817 49 22.371514947934163 26.90628149143433 29.123278468256636 21.598925092374873 50 19.717836748404434 26.2344642257306 31.239502855223378 22.808196170641583 51 19.01242861941552 27.133019818609338 28.812562982868663 25.041988579106484 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 107.0 1 58.5 2 10.0 3 8.5 4 7.0 5 6.5 6 6.0 7 11.5 8 17.0 9 15.0 10 13.0 11 25.5 12 38.0 13 62.0 14 86.0 15 93.0 16 100.0 17 101.0 18 102.0 19 96.0 20 90.0 21 86.0 22 82.0 23 89.5 24 97.0 25 98.5 26 111.0 27 122.0 28 174.5 29 227.0 30 313.0 31 399.0 32 484.0 33 569.0 34 649.5 35 730.0 36 731.0 37 732.0 38 721.0 39 710.0 40 719.5 41 729.0 42 740.0 43 751.0 44 750.5 45 750.0 46 747.5 47 745.0 48 742.0 49 739.0 50 725.0 51 711.0 52 828.0 53 945.0 54 745.5 55 546.0 56 502.0 57 458.0 58 411.5 59 365.0 60 338.5 61 312.0 62 250.0 63 188.0 64 159.0 65 130.0 66 111.0 67 92.0 68 70.5 69 49.0 70 34.0 71 19.0 72 19.5 73 20.0 74 13.5 75 5.5 76 4.0 77 4.0 78 4.0 79 2.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02519314746388982 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.008397715821296607 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 11908.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.53349566337519 #Duplication Level Percentage of deduplicated Percentage of total 1 96.99659444172337 87.81440762251925 2 2.5365653153098333 4.592882499469415 3 0.2042366217231491 0.5547076592122535 4 0.08788460597091155 0.31826002374179835 5 0.03749014654091207 0.16970570096404813 6 0.024960626486836144 0.13558636618806647 7 0.024951348158963126 0.15812529392413968 8 0.0 0.0 9 0.0 0.0 >10 0.04989536845263389 1.2874025919660672 >50 0.012473841877793643 0.5985292700608951 >100 0.024947683755587285 4.370392971954083 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 286 2.4017467248908297 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.8481692979509573 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 0.44507893852872016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 0.36110178031575413 No Hit TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT 30 0.2519314746388982 Illumina PCR Primer Index 8 (95% over 21bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28 0.23513604299630503 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.10917030567685589 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.008397715821296607 27 0.0 0.0 0.02519314746388982 28 0.0 0.0 0.05038629492777964 29 0.0 0.0 0.10077258985555929 30 0.0 0.0 0.15115888478333894 31 0.0 0.0 0.27712462210278804 32 0.0 0.0 0.49546523345649984 33 0.0 0.0 0.7893852872018811 34 0.0 0.0 1.0329190460194828 35 0.0 0.0 1.368827678871347 36 0.0 0.0 1.839099764863957 37 0.0 0.0 2.3261672824991604 38 0.0 0.0 3.056768558951965 39 0.0 0.0 3.6446086664427275 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACAT 20 6.755321E-4 45.0 5 GTATCAA 35 5.7436064E-6 38.571426 1 AGCCCAC 25 0.0020245952 36.0 17 AATCTCG 25 0.0020245952 36.0 36 ATCTCGT 25 0.0020245952 36.0 37 ATCTCCG 25 0.0020245952 36.0 10 GCCCACG 25 0.0020245952 36.0 18 TGCCGTC 25 0.0020245952 36.0 45 CCACGAG 25 0.0020245952 36.0 20 TATGCCG 25 0.0020245952 36.0 43 CACGAGA 25 0.0020245952 36.0 21 CCCACGA 25 0.0020245952 36.0 19 ACACATC 25 0.0020245952 36.0 6 TCTCGTA 25 0.0020245952 36.0 38 ACGAGAC 25 0.0020245952 36.0 22 ATGCCGT 25 0.0020245952 36.0 44 CGTATGC 25 0.0020245952 36.0 41 CGAGCCC 25 0.0020245952 36.0 15 CCGAGCC 25 0.0020245952 36.0 14 TCGTATG 25 0.0020245952 36.0 40 >>END_MODULE