FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_73.34100000006ca2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_73.34100000006ca2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11908
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC2862.4017467248908297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.8481692979509573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.44507893852872016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.36110178031575413No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT300.2519314746388982Illumina PCR Primer Index 8 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.23513604299630503No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10917030567685589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT206.755321E-445.05
GTATCAA355.7436064E-638.5714261
AGCCCAC250.002024595236.017
AATCTCG250.002024595236.036
ATCTCGT250.002024595236.037
ATCTCCG250.002024595236.010
GCCCACG250.002024595236.018
TGCCGTC250.002024595236.045
CCACGAG250.002024595236.020
TATGCCG250.002024595236.043
CACGAGA250.002024595236.021
CCCACGA250.002024595236.019
ACACATC250.002024595236.06
TCTCGTA250.002024595236.038
ACGAGAC250.002024595236.022
ATGCCGT250.002024595236.044
CGTATGC250.002024595236.041
CGAGCCC250.002024595236.015
CCGAGCC250.002024595236.014
TCGTATG250.002024595236.040
Produced by FastQC (version 0.11.2)