##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_72.34100000006c95.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 46467 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.29545699098285 38.0 38.0 38.0 32.0 38.0 2 36.35597305614737 38.0 38.0 38.0 32.0 38.0 3 36.292099769729056 38.0 38.0 38.0 32.0 38.0 4 36.33111670647987 38.0 38.0 38.0 32.0 38.0 5 36.39933716400887 38.0 38.0 38.0 32.0 38.0 6 38.2311963328814 40.0 38.0 40.0 38.0 40.0 7 38.13398756106484 40.0 38.0 40.0 38.0 40.0 8 38.150644543439434 40.0 38.0 40.0 38.0 40.0 9 38.14986980007317 40.0 38.0 40.0 38.0 40.0 10 38.104482751199775 40.0 38.0 40.0 38.0 40.0 11 38.03185056061291 40.0 38.0 40.0 32.0 40.0 12 37.914692147115154 40.0 38.0 40.0 32.0 40.0 13 37.96935459573461 40.0 38.0 40.0 32.0 40.0 14 37.87074698172897 40.0 38.0 40.0 32.0 40.0 15 37.90580411905223 40.0 38.0 40.0 32.0 40.0 16 37.91619859254955 40.0 38.0 40.0 32.0 40.0 17 37.89349430778832 40.0 38.0 40.0 32.0 40.0 18 37.84393225299675 40.0 38.0 40.0 32.0 40.0 19 37.93078959261411 40.0 38.0 40.0 32.0 40.0 20 37.933522715045086 40.0 38.0 40.0 32.0 40.0 21 37.98515075214669 40.0 38.0 40.0 32.0 40.0 22 37.977338756536895 40.0 38.0 40.0 32.0 40.0 23 37.913056577786385 40.0 38.0 40.0 32.0 40.0 24 37.915014956851095 40.0 38.0 40.0 32.0 40.0 25 37.90812834915101 40.0 38.0 40.0 32.0 40.0 26 37.822433124583036 40.0 38.0 40.0 32.0 40.0 27 37.74650827468957 40.0 38.0 40.0 32.0 40.0 28 37.73635052833193 40.0 38.0 40.0 32.0 40.0 29 37.753265758495274 40.0 38.0 40.0 32.0 40.0 30 37.72759162416339 40.0 38.0 40.0 32.0 40.0 31 37.696881657950804 40.0 38.0 40.0 32.0 40.0 32 37.67131512686423 40.0 38.0 40.0 32.0 40.0 33 37.67800804872275 40.0 38.0 40.0 32.0 40.0 34 37.70678115651968 40.0 38.0 40.0 32.0 40.0 35 37.721630404372995 40.0 38.0 40.0 32.0 40.0 36 37.675576215378655 40.0 38.0 40.0 32.0 40.0 37 37.63834549250005 40.0 38.0 40.0 32.0 40.0 38 37.62840295263305 40.0 38.0 40.0 32.0 40.0 39 37.564228377127854 40.0 38.0 40.0 32.0 40.0 40 37.56257128715002 40.0 38.0 40.0 32.0 40.0 41 37.53799470592033 40.0 38.0 40.0 32.0 40.0 42 37.60303010738804 40.0 38.0 40.0 32.0 40.0 43 37.5610218004175 40.0 38.0 40.0 32.0 40.0 44 37.538360556954395 40.0 38.0 40.0 32.0 40.0 45 37.50775819398713 40.0 38.0 40.0 32.0 40.0 46 37.47967374696021 40.0 38.0 40.0 32.0 40.0 47 37.43983902554501 40.0 38.0 40.0 32.0 40.0 48 37.443239288096926 40.0 38.0 40.0 32.0 40.0 49 37.38362709019304 40.0 38.0 40.0 32.0 40.0 50 37.35268039684077 40.0 38.0 40.0 32.0 40.0 51 36.75266318032152 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.08022934010899263 2201 2 -0.605422558578276 2201 3 -0.3445480864223569 2201 4 -0.059244138200575946 2201 5 0.3894941324338319 2201 6 0.1670400658806912 2201 7 0.02888682072176607 2201 8 -0.8364671031096691 2201 9 -0.3628948629257991 2201 10 -0.31374849067621113 2201 11 -0.43847995931626826 2201 12 -0.5409851968917678 2201 13 -0.7123068515291138 2201 14 -1.7963708516628287 2201 15 -0.43028028162977705 2201 16 -0.37317629293274734 2201 17 0.04510442479305965 2201 18 -0.43732311582931516 2201 19 -0.7808430233223689 2201 20 -0.8409975073184768 2201 21 -1.070916723070873 2201 22 0.08559963098349499 2201 23 -0.5496017914811446 2201 24 -0.8940316057394497 2201 25 -0.7362102873193095 2201 26 -0.4131894964115119 2201 27 -1.0573500835023992 2201 28 -0.6110643301481389 2201 29 -0.08658593902548972 2201 30 -1.2038753460517242 2201 31 0.07777929244139159 2201 32 -0.7763148471764438 2201 33 -2.360706642632067 2201 34 -2.053015599423688 2201 35 -1.8732668482473258 2201 36 -1.331996669112577 2201 37 -0.5757030452227312 2201 38 -0.35669627631170897 2201 39 -0.07011051907443289 2201 40 -0.2134693572073445 2201 41 0.08149513605453507 2201 42 -1.2187238548878696 2201 43 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2202 14 0.22873160735356635 2202 15 0.7764307839439937 2202 16 -0.18285866998192546 2202 17 -0.07912713258399151 2202 18 0.16769737597396528 2202 19 0.10056066520222373 2202 20 0.826266836943816 2202 21 0.5586939326668343 2202 22 0.08098897524578774 2202 23 0.46474247081393827 2202 24 0.7458249516376014 2202 25 0.23817495858232718 2202 26 0.39418755276881257 2202 27 0.12681999846481062 2202 28 -0.5234618711317438 2202 29 -0.22977241443532392 2202 30 -0.3550023952320487 2202 31 -0.11842972395204754 2202 32 0.850222038069461 2202 33 0.5888322917941622 2202 34 -0.6900545338499171 2202 35 0.14543192224447665 2202 36 0.010472593182505818 2202 37 -0.7030083730915848 2202 38 0.30057831385222755 2202 39 0.29669275961408914 2202 40 0.9079445772188848 2202 41 0.7359521032676497 2202 42 -0.2371664778386915 2202 43 -0.0031111356854296446 2202 44 -0.2407499607716872 2202 45 0.13914573662563612 2202 46 -0.184438596824954 2202 47 0.5129900010082054 2202 48 0.6133863056723712 2202 49 1.3626617134256733 2202 50 0.9805111599567056 2202 51 0.7363628612636006 1102 1 -0.7394427910385488 1102 2 0.394577441421724 1102 3 0.5898781430858406 1102 4 0.30141159950434115 1102 5 -0.495751769205512 1102 6 0.4129417052249522 1102 7 0.22560813219717346 1102 8 0.09795912639852844 1102 9 -0.002239125220881988 1102 10 0.9485465912910058 1102 11 0.004142991503407245 1102 12 0.22950660638692 1102 13 -0.023782261365184354 1102 14 0.6462520991568468 1102 15 -0.20077208490846488 1102 16 0.6268237070672527 1102 17 -0.1352234440593989 1102 18 0.3495621300723286 1102 19 -0.15789220365023482 1102 20 0.929494295960211 1102 21 0.5520340966012611 1102 22 0.3806815981966096 1102 23 0.4667916511418042 1102 24 -0.1235398024607619 1102 25 0.39493725366429544 1102 26 0.4392695199819272 1102 27 0.3196990968254667 1102 28 0.5528700960813708 1102 29 0.09374192982696883 1102 30 0.7141574408335245 1102 31 -0.18451578952581826 1102 32 0.33843925118421225 1102 33 -0.016444347550098826 1102 34 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42 0.1698848042125931 1214 43 0.24087924892995716 1214 44 0.5437051674334441 1214 45 -1.054363878758977 1214 46 0.078381915995557 1214 47 1.0442400010082054 1214 48 -0.6342098481737821 1214 49 0.4163476108615711 1214 50 0.15238615995670557 1214 51 0.20791734844308962 1215 1 0.7657912599193821 1215 2 0.418278804250086 1215 3 -0.248162544253681 1215 4 -0.7027361389906233 1215 5 0.3091070101743014 1215 6 0.4670598170163771 1215 7 0.6668485035185228 1215 8 -0.25301855481814073 1215 9 -0.2625590936190676 1215 10 -0.03367738658771913 1215 11 -0.06478847205575278 1215 12 0.03476040848053685 1215 13 0.7309086333612669 1215 14 0.8194695591607939 1215 15 -0.06863698714034427 1215 16 0.682916932427716 1215 17 0.6277629276569741 1215 18 0.29307388199806184 1215 19 0.32364048447933413 1215 20 0.44618400561851246 1215 21 -0.12918257335726224 1215 22 -0.28986945848915013 1215 23 0.3186581334645382 1215 24 -0.09114793992866055 1215 25 0.3639279706305203 1215 26 0.32491044433507454 1215 27 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-0.2722635941144489 1213 33 -0.8050254668265282 1213 34 -0.8270875798269302 1213 35 -1.141026411088859 1213 36 -1.1983995332542747 1213 37 -1.4409753271145718 1213 38 -0.7141486976420239 1213 39 -0.6472727576272916 1213 40 -2.243995077953528 1213 41 -1.5429631266174084 1213 42 -1.2788331445053558 1213 43 -1.0805177448808294 1213 44 -1.1477183515762874 1213 45 -1.2205884587766604 1213 46 0.2092395640945881 1213 47 -1.0189783897963949 1213 48 -0.675765993178203 1213 49 -0.21672047048237175 1213 50 -0.4395678630317974 1213 51 -1.1815609893111088 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 4.0 17 0.0 18 0.0 19 0.0 20 5.0 21 6.0 22 12.0 23 39.0 24 76.0 25 147.0 26 219.0 27 318.0 28 472.0 29 555.0 30 699.0 31 859.0 32 1158.0 33 1298.0 34 1518.0 35 1975.0 36 2670.0 37 4076.0 38 7338.0 39 23023.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.461621272472815 29.174292173538596 16.189040800947357 26.175045753041232 2 9.392015495534272 31.1546325191004 43.01517271064242 16.438179274722913 3 16.334172638646784 32.39288096929003 33.516258850366924 17.75668754169626 4 14.164891213118988 30.163341726386466 31.35128155465169 24.320485505842857 5 10.992747541265844 37.943056362579895 39.06428217875051 11.999913917403749 6 29.30681989368799 44.22708588891041 15.615382959950072 10.850711257451524 7 18.357113650547703 31.148987453461597 26.741558525405125 23.752340370585575 8 19.55581380334431 44.99752512535778 22.17057266447156 13.27608840682635 9 20.098134159726257 17.121828394344373 19.29971807949728 43.48031936643209 10 12.195751823875007 32.19919512772505 38.02268276411216 17.582370284287773 11 28.645833333333332 21.418732782369148 27.765581955922862 22.169851928374655 12 18.78322250199066 22.235134611659888 34.98827124626079 23.993371640088665 13 33.0966061936428 18.26887898939032 29.73723287494351 18.897281942023373 14 18.09240966707556 22.652635203477736 26.82548905674995 32.42946607269675 15 23.894376654399895 28.00912475520262 26.014160587083307 22.08233800331418 16 24.8224508242586 28.47673567769982 30.643911677355483 16.056901820686093 17 17.1670217573762 35.54996018679923 33.40865560505305 13.874362450771516 18 26.429509113994875 23.739427981147912 32.93520132567198 16.89586157918523 19 17.6856693997891 35.8318806895216 24.27314007790475 22.20930983278456 20 17.543633115974778 26.39507607549444 31.770934211375817 24.290356597154968 21 13.81625669830202 32.27451739944477 24.195665741278756 29.713560160974456 22 17.935308928917294 37.03058084231821 24.361374739062132 20.672735489702372 23 14.242365549744981 25.67198226698517 26.806120472593452 33.279531710676395 24 21.80687369531065 34.10807669959326 29.042115910215855 15.042933694880237 25 21.09884434114533 31.618137603030107 25.56653108657757 21.716486969246994 26 19.47230232858434 29.533422287263804 32.072913528171135 18.921361855980717 27 21.350635935179803 32.93089719585943 29.515570189596918 16.20289667936385 28 14.872920567284309 25.33410807669959 31.123162674586265 28.66980868142983 29 23.151914261734134 27.395786256913507 31.751565627219318 17.70073385413304 30 16.56300441192295 30.726353169051972 32.108038308404176 20.6026041106209 31 19.8441905007855 25.82693094023716 29.509113994878085 24.819764564099252 32 22.768846708416728 27.64327372113543 30.910108248864788 18.67777132158306 33 15.882239008328492 30.910108248864788 32.007661351066346 21.199991391740376 34 14.425291066778573 30.03206576710354 38.4229668366798 17.119676329438096 35 13.084554630167647 36.968170960036154 31.775238341188373 18.17203606860783 36 15.051541954505348 27.854176081950634 38.14535046376999 18.948931499774034 37 13.669916284675146 32.605935395011514 33.367766371833774 20.356381948479566 38 15.948953020423096 26.513439645339705 36.479652226311146 21.057955107926055 39 15.034325435255127 23.752340370585575 37.08007833516259 24.13325585899671 40 14.99558826694213 23.720059396991413 37.131727892913254 24.152624443153208 41 15.789700217358554 22.078033873501624 36.76157272903351 25.37069318010631 42 19.84634256569178 24.802548044849033 34.92586136397874 20.42524802548045 43 12.118277487249015 26.866378289969223 39.25581595540922 21.759528267372545 44 11.203649902081047 31.932339079346633 37.09514278950653 19.768868229065788 45 12.064475864592076 29.36062151634493 36.56143069274969 22.013471926313297 46 17.38007618309768 25.17700733854133 33.230034217832014 24.21288226052898 47 13.601050207674264 28.349151010394475 31.89790604084619 26.15189274108507 48 10.654873350980266 25.61818064432823 28.90868788602664 34.81825811866486 49 18.80904728086599 28.684873135773774 30.298921815481954 22.20715776787828 50 10.827038543482471 27.303247465943574 35.53489573245529 26.334818258118663 51 10.56233456001033 28.22002711601782 27.68631501926098 33.53132330471087 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 137.0 1 81.5 2 26.0 3 52.5 4 79.0 5 76.5 6 74.0 7 84.0 8 94.0 9 78.5 10 63.0 11 127.5 12 192.0 13 330.0 14 468.0 15 498.0 16 528.0 17 376.0 18 224.0 19 187.0 20 150.0 21 236.5 22 323.0 23 210.5 24 98.0 25 103.5 26 208.0 27 307.0 28 258.5 29 210.0 30 474.0 31 738.0 32 1272.5 33 1807.0 34 3327.5 35 4848.0 36 5681.5 37 6515.0 38 7598.0 39 8681.0 40 7999.0 41 7317.0 42 5582.0 43 3847.0 44 3058.0 45 2269.0 46 1697.5 47 1126.0 48 878.0 49 630.0 50 745.0 51 860.0 52 2078.5 53 3297.0 54 1825.0 55 353.0 56 314.5 57 276.0 58 251.0 59 226.0 60 216.5 61 207.0 62 169.0 63 131.0 64 106.0 65 81.0 66 67.0 67 53.0 68 53.0 69 53.0 70 41.0 71 29.0 72 25.5 73 22.0 74 16.0 75 9.0 76 8.0 77 4.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047345427938106616 2 0.004304129812555147 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.006456194718832719 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0021520649062775734 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0021520649062775734 27 0.0 28 0.0 29 0.0 30 0.004304129812555147 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 46467.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.953439706758765 #Duplication Level Percentage of deduplicated Percentage of total 1 92.01704324229377 58.84806426990248 2 4.826492000084033 6.173415302450555 3 1.0028974462411153 1.9241622408073051 4 0.4657447708852732 1.1914392049419795 5 0.27777289962135826 0.8882266194053045 6 0.2033550621539577 0.7803153423916388 7 0.13367034453804105 0.5984074823996696 8 0.12099685818228822 0.6190532219574569 9 0.09187740152675922 0.5288288273059735 >10 0.6943748604186172 9.375506214705794 >50 0.0879623068731379 3.8361528529550437 >100 0.07442964165202426 9.085169403414135 >500 0.0 0.0 >1k 0.0033831655296373284 6.15125901736266 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 2843 6.118320528547141 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACA 449 0.9662771429186305 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC 442 0.9512126885746874 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACATC 306 0.6585318613209374 No Hit TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 279 0.600426108851443 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 278 0.5982740439451654 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC 275 0.5918178492263327 No Hit TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT 259 0.5573848107258914 Illumina PCR Primer Index 11 (95% over 21bp) TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 173 0.3723072287860202 No Hit AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC 171 0.36800309897346506 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 155 0.33357006047302384 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACA 132 0.2840725676286397 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG 131 0.2819205027223621 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 131 0.2819205027223621 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 126 0.2711601781909742 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.2711601781909742 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTATAC 123 0.2647039834721415 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACA 117 0.2517915940344761 No Hit CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG 109 0.2345750747842555 No Hit GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC 107 0.23027094497170036 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTAT 105 0.22596681515914518 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 104 0.22381475025286765 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 101 0.21735855553403494 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAACTGTCTCTTATA 98 0.2109023608152022 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAACTGTCTCTTATACACA 83 0.17862138722103857 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA 81 0.17431725740848344 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACATC 81 0.17431725740848344 No Hit TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 81 0.17431725740848344 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 79 0.1700131275959283 No Hit AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC 77 0.16570899778337317 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC 74 0.15925280306454043 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCC 71 0.1527966083457077 No Hit AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA 68 0.146340413626875 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAACTGTCTCTTA 67 0.1441883487205974 No Hit TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 66 0.14203628381431985 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG 66 0.14203628381431985 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64 0.1377321540017647 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGACTGTCTCTTATACACATC 63 0.1355800890954871 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 63 0.1355800890954871 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.13342802418920954 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACATC 61 0.13127595928293195 No Hit TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT 61 0.13127595928293195 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61 0.13127595928293195 No Hit CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 60 0.1291238943766544 No Hit TCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC 59 0.12697182947037683 No Hit TTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATAC 59 0.12697182947037683 No Hit TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACAC 59 0.12697182947037683 No Hit AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA 56 0.12051563475154412 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTC 53 0.11405944003271139 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACAC 50 0.10760324531387867 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCCAAACTGTCTCTTATACACATC 50 0.10760324531387867 No Hit TTTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATA 50 0.10760324531387867 No Hit TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 49 0.1054511804076011 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48 0.10329911550132351 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48 0.10329911550132351 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47 0.10114705059504595 No Hit TTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT 47 0.10114705059504595 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0021520649062775734 4 0.0 0.0 0.004304129812555147 5 0.0 0.0 0.004304129812555147 6 0.0 0.0 0.004304129812555147 7 0.0 0.0 0.004304129812555147 8 0.0 0.0 0.004304129812555147 9 0.0 0.0 0.008608259625110293 10 0.0 0.0 0.008608259625110293 11 0.0 0.0 0.010760324531387866 12 0.0 0.0 0.010760324531387866 13 0.0 0.0 0.010760324531387866 14 0.0 0.0 0.01291238943766544 15 0.0 0.0 0.021520649062775732 16 0.0 0.0 0.04519336303182904 17 0.0 0.0 0.06025781737577205 18 0.0 0.0 0.07532227171971506 19 0.0 0.0 0.09469085587621323 20 0.0 0.0 0.10975531022015624 21 0.0 0.0 0.1850775819398713 22 0.0 0.0 0.2797684378160845 23 0.0 0.0 0.5746013299761121 24 0.0 0.0 1.0459035444509006 25 0.0 0.0 1.1578109195773345 26 0.0 0.0 1.8809047280865991 27 0.0 0.0 2.087502959089246 28 0.0 0.0 2.86439839025545 29 0.0 0.0 3.6864871844534832 30 0.0 0.0 4.153485269115716 31 0.0 0.0 6.753179675899025 32 0.0 0.0 8.365076290700928 33 0.0 0.0 9.548711989153594 34 0.0 0.0 13.17924548604386 35 0.0 0.0 14.761013192157876 36 0.0 0.0 19.185658639464567 37 0.0 0.0 21.705726644715604 38 0.0 0.0 24.111735209933933 39 0.0 0.0 26.857770030344117 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 270 0.0 40.000004 45 ACGCTAT 275 0.0 39.272728 30 GACGCTA 275 0.0 39.272728 29 AGACGAC 275 0.0 39.272728 25 GACGACG 275 0.0 39.272728 26 CGACGCT 275 0.0 39.272728 28 ACGACGC 275 0.0 39.272728 27 GAGACGA 275 0.0 39.272728 24 CCACGAG 280 0.0 38.571426 20 CACGAGA 280 0.0 38.571426 21 CGCTATA 280 0.0 38.571426 31 GCTATAT 280 0.0 38.571426 32 ACGAGAC 280 0.0 38.571426 22 CGAGACG 280 0.0 38.571426 23 CTATATC 275 0.0 38.454544 33 TCTCGTA 275 0.0 38.454544 38 TATCTCG 275 0.0 38.454544 36 ATATCTC 275 0.0 38.454544 35 CTCGTAT 275 0.0 38.454544 39 GTATGCC 285 0.0 37.894737 42 >>END_MODULE