FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_72.34100000006c95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_72.34100000006c95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46467
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC28436.118320528547141No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACA4490.9662771429186305No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC4420.9512126885746874No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACATC3060.6585318613209374No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA2790.600426108851443No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.5982740439451654No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC2750.5918178492263327No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT2590.5573848107258914Illumina PCR Primer Index 11 (95% over 21bp)
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC1730.3723072287860202No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC1710.36800309897346506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.33357006047302384No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACA1320.2840725676286397No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG1310.2819205027223621No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.2819205027223621No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1260.2711601781909742No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.2711601781909742No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTATAC1230.2647039834721415No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACA1170.2517915940344761No Hit
CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG1090.2345750747842555No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC1070.23027094497170036No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTAT1050.22596681515914518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.22381475025286765No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT1010.21735855553403494No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAACTGTCTCTTATA980.2109023608152022No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAACTGTCTCTTATACACA830.17862138722103857No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA810.17431725740848344No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACATC810.17431725740848344No Hit
TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA810.17431725740848344No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.1700131275959283No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC770.16570899778337317No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC740.15925280306454043No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCC710.1527966083457077No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA680.146340413626875No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAACTGTCTCTTA670.1441883487205974No Hit
TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA660.14203628381431985No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG660.14203628381431985No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.1377321540017647No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGACTGTCTCTTATACACATC630.1355800890954871No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.1355800890954871No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.13342802418920954No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACATC610.13127595928293195No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT610.13127595928293195No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.13127595928293195No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC600.1291238943766544No Hit
TCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC590.12697182947037683No Hit
TTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATAC590.12697182947037683No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACAC590.12697182947037683No Hit
AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA560.12051563475154412No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTC530.11405944003271139No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACAC500.10760324531387867No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCAAACTGTCTCTTATACACATC500.10760324531387867No Hit
TTTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATA500.10760324531387867No Hit
TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC490.1054511804076011No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.10329911550132351No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.10329911550132351No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.10114705059504595No Hit
TTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT470.10114705059504595No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2700.040.00000445
ACGCTAT2750.039.27272830
GACGCTA2750.039.27272829
AGACGAC2750.039.27272825
GACGACG2750.039.27272826
CGACGCT2750.039.27272828
ACGACGC2750.039.27272827
GAGACGA2750.039.27272824
CCACGAG2800.038.57142620
CACGAGA2800.038.57142621
CGCTATA2800.038.57142631
GCTATAT2800.038.57142632
ACGAGAC2800.038.57142622
CGAGACG2800.038.57142623
CTATATC2750.038.45454433
TCTCGTA2750.038.45454438
TATCTCG2750.038.45454436
ATATCTC2750.038.45454435
CTCGTAT2750.038.45454439
GTATGCC2850.037.89473742
Produced by FastQC (version 0.11.2)